Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice
Abstract: Maternal eating habits during the preconceptional period affect different aspects of health, raising questions about the impact of diet on their reproductive capacity. Objective: To analyze, in mice, the effects of the consumption of a high fat diet prior to mating, on morph...
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35104 |
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I10-R327-article-35104 |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
Nutrition high fat diet reproduction fetal development nutrición dieta grasa reproducción desarrollo fetal . |
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Nutrition high fat diet reproduction fetal development nutrición dieta grasa reproducción desarrollo fetal . Romero , FE Santillán, ME Solís, MR Luque, EM Cantarelli, VI Torres, PJ Bianconi, S Vincenti, L Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| topic_facet |
Nutrition high fat diet reproduction fetal development nutrición dieta grasa reproducción desarrollo fetal . |
| author |
Romero , FE Santillán, ME Solís, MR Luque, EM Cantarelli, VI Torres, PJ Bianconi, S Vincenti, L |
| author_facet |
Romero , FE Santillán, ME Solís, MR Luque, EM Cantarelli, VI Torres, PJ Bianconi, S Vincenti, L |
| author_sort |
Romero , FE |
| title |
Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| title_short |
Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| title_full |
Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| title_fullStr |
Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| title_full_unstemmed |
Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| title_sort |
effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice |
| description |
Abstract:
Maternal eating habits during the preconceptional period affect different aspects of health, raising questions about the impact of diet on their reproductive capacity. Objective: To analyze, in mice, the effects of the consumption of a high fat diet prior to mating, on morphometric, biochemical and reproductive parameters. Female Albino Swiss N:NIH adults mice (light/dark cycles: 14/10 h, temperature: 24±2 ºC, water and food ad libitum), were fed a high fat diet (HF: 24% fat, 24% protein, 41% carbohydrates) or control (C: 4% fat, 19% protein, 64% carbohydrate), for 10 weeks. Body weight was recorded during treatment. In 18 animals (9/group) we evaluated: weight of retroperitoneal fatty pads, ovaries and liver; nitrite concentration in ovaries; oocyte quality; plasma concentration of progesterone and estrogen; plasma lipid profile and glycemia. Other females (14 C and 12 HF) were mated with control males; interrupting the pregnancy on day 14, we evaluated: weight and size of fetuses, placentas and fetoplacental units (FPU); number of corpora lutea (both ovaries) and viable fetuses. ANOVA and Chi square were used, considering significant differences, values of p≤0.05. Body weight in HF was significantly higher than in C, from day 29 of treatment (p<0.05). Significant differences (p <0.05) were recorded in: fat pad weights, absolute (0.55±0.05 g -C- and 2.17±0.54 g -HF) and relative (2.00±0.17% -C- and 5.87±1.24% -HF); glycemia (87.91±5.81 mg / dl –C- and 129.03±16.38 mg/dl –HF-); oocyte quality (radiated crown, zona pellucida, ooplasm, granular cells, perivithelial space, first polar body), with deleterious effects observed in HF; fetal weight (0.23±0.01 g and 0.20±0.02 g), fetal length (12.32±0.18 mm and 10.87±0.15 mm), placental diameter (7.16±0.13 mm and 6.45±0.11 mm) and weight of FPU (0.54±0.01 g and 0.46±0.01 g), in C and HF respectively. The rest of the parameters did not change significantly. These results suggest that the consumption of the HF diet, under the experimental conditions described, can modify the body weight of female mice, as well as fundamental metabolic parameters, such as glycemia and others at the reproductive level, such as oocyte quality and fetal development.
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35104 |
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I10-R327-article-351042024-04-15T16:19:09Z Effects of consuming high-fat diet prior to pregnancy on reproductive physiology and fetal development in mice Efectos del consumo de dieta hipergrasa previo a la gestación, sobre la fisiología reproductiva y el desarrollo fetal en ratones A Romero , FE Santillán, ME Solís, MR Luque, EM Cantarelli, VI Torres, PJ Bianconi, S Vincenti, L Nutrition high fat diet reproduction fetal development nutrición dieta grasa reproducción desarrollo fetal . Abstract: Maternal eating habits during the preconceptional period affect different aspects of health, raising questions about the impact of diet on their reproductive capacity. Objective: To analyze, in mice, the effects of the consumption of a high fat diet prior to mating, on morphometric, biochemical and reproductive parameters. Female Albino Swiss N:NIH adults mice (light/dark cycles: 14/10 h, temperature: 24±2 ºC, water and food ad libitum), were fed a high fat diet (HF: 24% fat, 24% protein, 41% carbohydrates) or control (C: 4% fat, 19% protein, 64% carbohydrate), for 10 weeks. Body weight was recorded during treatment. In 18 animals (9/group) we evaluated: weight of retroperitoneal fatty pads, ovaries and liver; nitrite concentration in ovaries; oocyte quality; plasma concentration of progesterone and estrogen; plasma lipid profile and glycemia. Other females (14 C and 12 HF) were mated with control males; interrupting the pregnancy on day 14, we evaluated: weight and size of fetuses, placentas and fetoplacental units (FPU); number of corpora lutea (both ovaries) and viable fetuses. ANOVA and Chi square were used, considering significant differences, values of p≤0.05. Body weight in HF was significantly higher than in C, from day 29 of treatment (p<0.05). Significant differences (p <0.05) were recorded in: fat pad weights, absolute (0.55±0.05 g -C- and 2.17±0.54 g -HF) and relative (2.00±0.17% -C- and 5.87±1.24% -HF); glycemia (87.91±5.81 mg / dl –C- and 129.03±16.38 mg/dl –HF-); oocyte quality (radiated crown, zona pellucida, ooplasm, granular cells, perivithelial space, first polar body), with deleterious effects observed in HF; fetal weight (0.23±0.01 g and 0.20±0.02 g), fetal length (12.32±0.18 mm and 10.87±0.15 mm), placental diameter (7.16±0.13 mm and 6.45±0.11 mm) and weight of FPU (0.54±0.01 g and 0.46±0.01 g), in C and HF respectively. The rest of the parameters did not change significantly. These results suggest that the consumption of the HF diet, under the experimental conditions described, can modify the body weight of female mice, as well as fundamental metabolic parameters, such as glycemia and others at the reproductive level, such as oocyte quality and fetal development. Resumen: Los hábitos alimentarios maternos durante el período preconcepcional afectan diferentes aspectos de la salud, planteándose interrogantes sobre el impacto de la dieta en su capacidad reproductiva. Objetivo: Analizar, en ratones, los efectos del consumo de dieta hipergrasa previo al apareamiento, sobre parámetros morfométricos, bioquímicos y reproductivos. Ratones hembras Albino Swiss N:NIH adultas (ciclos luz/oscuridad: 14/10 h, temperatura: 24±2ºC, agua y comida ad libitum), fueron alimentadas con dieta hipergrasa (HG: 24% grasa, 24% proteínas, 41% carbohidratos) o control (C: 4% grasas, 19% proteínas, 64% carbohidratos), durante 10 semanas. Se registró el peso corporal durante el tratamiento. En 18 animales (9/grupo) se evaluó: peso de pads grasos retroperitoneales, ovarios e hígado; concentración de nitritos en ovarios; calidad ovocitaria; concentración plasmática de progesterona y estrógeno; perfil lipídico plasmático y glucemia. Otras hembras (14 C y 12 HG) fueron apareadas con machos controles; interrumpiéndose la gestación el día 14, se evaluó: peso y tamaño de fetos, placentas y unidades fetoplacentarias (UFP); número de cuerpos lúteos en ambos ovarios; cantidad de fetos viables. Se empleó ANOVA y Chi cuadrado, considerándose diferencias significativas, valores de p≤0.05. El peso corporal en las HG fue significativamente superior que en las C desde el día 29 del tratamiento (p<0,05). Se registraron diferencias significativas (p<0,05) en: pesos de pads grasos, absolutos (0,55±0,05 g -C- y 2,17±0,54 g -HG) y relativos (2,00±0,17 % -C- y 5,87±1,24 % -HG); glucemia (87,91±5,81 mg/dl –C- y 129,03±16,38 mg/dl –HG-); calidad ovocitaria (corona radiada, zona pelúcida, ooplasma, células granulosas, espacio perivitelino, primer cuerpo polar), observándose efectos deletéreos en las HG; peso fetal (0,23±0,01 g y 0,20±0,02 g), longitud fetal (12,32±0,18 mm y 10,87±0,15 mm), diámetro de placenta (7,16±0,13 mm y 6,45±0,11 mm) y peso de UFP (0,54±0,01 g y 0,46±0,01 g), en C e HG respectivamente. El resto de los parámetros no se modificó significativamente. Estos resultados sugieren que el consumo de dieta HG, en las condiciones experimentales descriptas, puede modificar el peso corporal de las hembras de ratón, así como parámetros metabólicos fundamentales, como los niveles de glucosa en sangre y otros a nivel reproductivo, como la calidad ovocitaria y el desarrollo fetal. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35104 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |