Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba.
Abstract: Although the nasopharyngeal swab (NPS) is considered the optimal sample for the diagnosis of SARS-CoV-2 infection, the nasal swab (NS) is usually used since it is less invasive and of greater adherence. Our aim was to evaluate the performance of the nasal antigen detection t...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35091 |
| Aporte de: |
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I10-R327-article-35091 |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
SARS-CoV-2 nasopharyngeal swab nasal swab Diagnosis SARS-CoV-2 hisopado nasofaríngeo hisopado nasal diagnóstico . |
| spellingShingle |
SARS-CoV-2 nasopharyngeal swab nasal swab Diagnosis SARS-CoV-2 hisopado nasofaríngeo hisopado nasal diagnóstico . Sicilia , P Castro , G Gierotto, R Lopez , L Barbás , MG Pisano , MB Re, VE Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| topic_facet |
SARS-CoV-2 nasopharyngeal swab nasal swab Diagnosis SARS-CoV-2 hisopado nasofaríngeo hisopado nasal diagnóstico . |
| author |
Sicilia , P Castro , G Gierotto, R Lopez , L Barbás , MG Pisano , MB Re, VE |
| author_facet |
Sicilia , P Castro , G Gierotto, R Lopez , L Barbás , MG Pisano , MB Re, VE |
| author_sort |
Sicilia , P |
| title |
Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| title_short |
Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| title_full |
Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| title_fullStr |
Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| title_full_unstemmed |
Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. |
| title_sort |
evaluation of the sars-cov-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from córdoba. |
| description |
Abstract:
Although the nasopharyngeal swab (NPS) is considered the optimal sample for the diagnosis of SARS-CoV-2 infection, the nasal swab (NS) is usually used since it is less invasive and of greater adherence.
Our aim was to evaluate the performance of the nasal antigen detection test as a massive testing strategy in outpatients in Córdoba.
Two hundred and forty-eight samples from persons (symptomatic n=126, asymptomatic n=122) who voluntarily agreed to the simultaneous collection of samples of NPS, NS and oropharyngeal swab (OPS) for the detection of the rapid antigen PanbioTM COVID-19 Ag Rapid Test (Ag-NS), PanbioTM COVID-19 Ag Rapid Test (Ag-NPS) and real time RT-PCR for RNA detection in OPS (DisCoVery SARS-CoV-2 Nucleic Acid Detection Kit) were included.
Fifty samples resulted positive for RNA-SARS-CoV-2 molecular detection, from which 45 and 40 were positive for Ag-NPS and Ag-NS, respectively. Five samples were positive only for Ag-NPS and negative for Ag-NS [symptomatic n=4 and asymptomatic n=1, mean Ct=30.7 (28.3-32.6)]. One sample was false positive for Ag-NPS. Five samples were false negative by both rapid Ag tests [symptomatic n=3, asymptomatic n=2, mean Cts=33.3 (30.8-36.2)]. The Ag-NPS rapid test yielded a sensitivity of 90.0% (95% CI: 80.7-99.3) and the Ag-NS rapid test a sensitivity of 80.0% (95% CI: 67.9 -92.1), kappa index = 0.89. In symptomatic patients, the Ag-NPS test presented a sensitivity of 91.9% (95% CI: 81.8-100.0), while the Ag-NS test had a sensitivity of 81.1% (95% CI: 67.1-95.1), kappa index=0.90. In asymptomatic patients, the sensitivity for the Ag-NPS test was 86.6% (95% CI: 61.2-100.0) and 76.9% (95% CI: 50.2-100.0) for Ag-NS, kappa index=0.82.
The results obtained show an adequate sensitivity of the Ag-NPS (90%) and Ag-NS (80%) tests for the detection of SARS-CoV-2 infection. Although the use of NS has lower sensitivity, in accordance with previous studies, the results found indicate that its use would be acceptable, due to its operative capacity as it is less invasive, increasing adherence and the evaluation of a greater number of patients, improving efficient contact management and early isolation.
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35091 |
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I10-R327-article-350912024-04-15T16:19:09Z Evaluation of the SARS-CoV-2 antigen detection technique in nasopharyngeal swab and nasal swab in ambulatory patients from Córdoba. Evaluación de la técnica de detección de antígeno de SARS-CoV-2 en hisopado nasofaríngeo e hisopado nasal en pacientes ambulatorios de Córdoba A Sicilia , P Castro , G Gierotto, R Lopez , L Barbás , MG Pisano , MB Re, VE SARS-CoV-2 nasopharyngeal swab nasal swab Diagnosis SARS-CoV-2 hisopado nasofaríngeo hisopado nasal diagnóstico . Abstract: Although the nasopharyngeal swab (NPS) is considered the optimal sample for the diagnosis of SARS-CoV-2 infection, the nasal swab (NS) is usually used since it is less invasive and of greater adherence. Our aim was to evaluate the performance of the nasal antigen detection test as a massive testing strategy in outpatients in Córdoba. Two hundred and forty-eight samples from persons (symptomatic n=126, asymptomatic n=122) who voluntarily agreed to the simultaneous collection of samples of NPS, NS and oropharyngeal swab (OPS) for the detection of the rapid antigen PanbioTM COVID-19 Ag Rapid Test (Ag-NS), PanbioTM COVID-19 Ag Rapid Test (Ag-NPS) and real time RT-PCR for RNA detection in OPS (DisCoVery SARS-CoV-2 Nucleic Acid Detection Kit) were included. Fifty samples resulted positive for RNA-SARS-CoV-2 molecular detection, from which 45 and 40 were positive for Ag-NPS and Ag-NS, respectively. Five samples were positive only for Ag-NPS and negative for Ag-NS [symptomatic n=4 and asymptomatic n=1, mean Ct=30.7 (28.3-32.6)]. One sample was false positive for Ag-NPS. Five samples were false negative by both rapid Ag tests [symptomatic n=3, asymptomatic n=2, mean Cts=33.3 (30.8-36.2)]. The Ag-NPS rapid test yielded a sensitivity of 90.0% (95% CI: 80.7-99.3) and the Ag-NS rapid test a sensitivity of 80.0% (95% CI: 67.9 -92.1), kappa index = 0.89. In symptomatic patients, the Ag-NPS test presented a sensitivity of 91.9% (95% CI: 81.8-100.0), while the Ag-NS test had a sensitivity of 81.1% (95% CI: 67.1-95.1), kappa index=0.90. In asymptomatic patients, the sensitivity for the Ag-NPS test was 86.6% (95% CI: 61.2-100.0) and 76.9% (95% CI: 50.2-100.0) for Ag-NS, kappa index=0.82. The results obtained show an adequate sensitivity of the Ag-NPS (90%) and Ag-NS (80%) tests for the detection of SARS-CoV-2 infection. Although the use of NS has lower sensitivity, in accordance with previous studies, the results found indicate that its use would be acceptable, due to its operative capacity as it is less invasive, increasing adherence and the evaluation of a greater number of patients, improving efficient contact management and early isolation. Resumen: Si bien el hisopado nasofaríngeo (HNF) es considerado la muestra óptima para el diagnóstico de la infección por SARS-CoV-2, el hisopado nasal (HN) es usualmente utilizado ya que resulta menos invasivo y de mayor adherencia. El objetivo fue evaluar el rendimiento de la prueba de detección de antígeno nasal como estrategia de testeos masivos en pacientes ambulatorios de Córdoba. Se incluyeron 248 muestras de personas (sintomáticas n=126, asintomáticas n=122) que accedieron voluntariamente a la toma simultánea de muestras de HNF, HN e hisopado orofaríngeo (HOF) para la detección de antígeno rápido PanbioTM COVID-19 Ag Rapid Test (Ag-HN), PanbioTM COVID-19 Ag Rapid Test (Ag-HNF) y qPCR para la detección de RNA en HOF (DisCoVery SARS-CoV-2 Nucleic Acid Detection Kit). Cincuenta muestras resultaron RNA-SARS-CoV-2 positivas, de las cuales 45 y 40 fueron positivas para las pruebas de Ag-HNF y Ag-HN, respectivamente. Cinco muestras resultaron positivas sólo para Ag-HNF y negativas para Ag-HN [sintomáticas n=4 y asintomáticas n=1, Cts promedio=30,7 (28,3-32.6)]. Una muestra resultó falsa positiva para Ag-HNF. Cinco muestras resultaron falsas negativas por ambos test rápidos de Ag [sintomáticas n=3, asintomáticas n=2, Cts promedio=33,3 (30,8-36,2)]. El test rápido de Ag-HNF arrojó una sensibilidad del 90,0% (IC 95%: 80,7-99,3) y el test rápido Ag-HN una sensibilidad del 80,0% (IC 95%: 67,9-92,1), índice kappa=0,89. En pacientes sintomáticos, el test de Ag-HNF presentó una sensibilidad del 91,9% (IC 95%: 81,8-100,0), mientras que el de Ag-HN una sensibilidad del 81,1% (IC 95%: 67,1-95,1), índice kappa=0,90. En pacientes asintomáticos, la sensibilidad para el test de Ag-HNF fue 86,6% (IC 95%: 61,2-100,0) y 76,9% (IC 95%: 50,2-100,0) para Ag-HN, índice kappa=0,82. Los resultados obtenidos muestran una adecuada sensibilidad de los tests Ag-HNF (90%) y Ag-HN (80%) para la detección de la infección por SARS-CoV-2. Si bien el uso de HN posee menor sensibilidad, en concordancia con estudios previos, los resultados hallados indican que su uso sería aceptable, debido a su capacidad operativa por ser menos invasivo, aumentando la adherencia y la evaluación de una mayor cantidad de pacientes, mejorando la eficiencia en el manejo de los contactos y el aislamiento precoz. . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35091 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |