Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome
The Metabolic Syndrome (MS) generates oxidative stress (OS) altering the male fertility. Quercetin (Qc) supplementation could improve this condition due to its antioxidant properties. Objective: to clarify the mechanisms by which MS affects testicular tissue and to determine if these alterations are...
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/35053 |
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I10-R327-article-35053 |
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ojs |
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Universidad Nacional de Córdoba |
| institution_str |
I-10 |
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R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
quercetin testicular oxidative stress Metabolic Syndrome quercetina Estrés oxidativo testicular síndrome metabólico |
| spellingShingle |
quercetin testicular oxidative stress Metabolic Syndrome quercetina Estrés oxidativo testicular síndrome metabólico Rivoira, MA Rodriguez, V Moine, L Silvano, L Luque, E Martini, C Tolosa de Talamoni, N Diaz de Barboza, G Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| topic_facet |
quercetin testicular oxidative stress Metabolic Syndrome quercetina Estrés oxidativo testicular síndrome metabólico |
| author |
Rivoira, MA Rodriguez, V Moine, L Silvano, L Luque, E Martini, C Tolosa de Talamoni, N Diaz de Barboza, G |
| author_facet |
Rivoira, MA Rodriguez, V Moine, L Silvano, L Luque, E Martini, C Tolosa de Talamoni, N Diaz de Barboza, G |
| author_sort |
Rivoira, MA |
| title |
Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| title_short |
Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| title_full |
Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| title_fullStr |
Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| title_full_unstemmed |
Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome |
| title_sort |
quercetin attenuates testicular oxidative stress associated with metabolic syndrome |
| description |
The Metabolic Syndrome (MS) generates oxidative stress (OS) altering the male fertility. Quercetin (Qc) supplementation could improve this condition due to its antioxidant properties. Objective: to clarify the mechanisms by which MS affects testicular tissue and to determine if these alterations are reversed by treatment with Qc. Four groups of male Wistar rats were used: Control (C, n: 5), C + Qc (n: 4, 50mg / kg / day, orally, 15 days), MS (n: 4, 10% fructose in water, 45 days) and MS + Qc (n: 4, SM 30 days plus 15 days with Qc treatment). The study time was 45 days. Body weight and abdominal circumference were measured and serum glucose, triglycerides, cholesterol, HDL-cholesterol and testosterone were measured. In epididymis samples, vitality, motility and number of spermatozoa were determined. Glutathione (GSH) and catalase activity (CAT) were quantified in the testis homogenates. The histology of the testes was analyzed with hematoxylin / PAS. ANOVA and Bonferroni were performed (significant differences at p <0.05). MS was confirmed by body weight gain, a decrease in waist circumference, blood glucose (mg/dL) (221.6±13.3 vs 143.0±13.0) and triglycerides (392.7±85.7 vs 86.9±21.9) and a decrease in HDL-cholesterol (25.0±1.1 vs 34.8±0.6). Qc normalized the biochemical parameters. Serum testosterone was similar in all groups. MS produced GSH depletion (nmol/mg prot.) (31.7±1.4 vs 45.1±0.5) and increased CAT activity (IU/mg prot.) (16.1±0.6 vs 9.8±1.6). Qc partially restored the tripeptide levels and normalized the enzyme activity. The area of the testicular sections and the thickness of the cell layer in the seminiferous tubules were lower in the MS group. At the end of the study, all groups presented complete spermatogenesis and the same concentration and motility of spermatozoa. The results suggest that MS produces OS and alters testicular tissue. Although complete spermatogenesis occurs during the development of MS, OS could be the mechanism by which fertility would be affected at longer times. Qc normalizes the testicular redox status and improves the general conditions of MS, which could prevent the development of male subfertility. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/35053 |
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I10-R327-article-350532024-04-15T16:19:09Z Quercetin attenuates testicular oxidative stress associated with Metabolic Syndrome Quercetina atenúa el estrés oxidativo testicular asociado al Síndrome Metabólico Rivoira, MA Rodriguez, V Moine, L Silvano, L Luque, E Martini, C Tolosa de Talamoni, N Diaz de Barboza, G quercetin testicular oxidative stress Metabolic Syndrome quercetina Estrés oxidativo testicular síndrome metabólico The Metabolic Syndrome (MS) generates oxidative stress (OS) altering the male fertility. Quercetin (Qc) supplementation could improve this condition due to its antioxidant properties. Objective: to clarify the mechanisms by which MS affects testicular tissue and to determine if these alterations are reversed by treatment with Qc. Four groups of male Wistar rats were used: Control (C, n: 5), C + Qc (n: 4, 50mg / kg / day, orally, 15 days), MS (n: 4, 10% fructose in water, 45 days) and MS + Qc (n: 4, SM 30 days plus 15 days with Qc treatment). The study time was 45 days. Body weight and abdominal circumference were measured and serum glucose, triglycerides, cholesterol, HDL-cholesterol and testosterone were measured. In epididymis samples, vitality, motility and number of spermatozoa were determined. Glutathione (GSH) and catalase activity (CAT) were quantified in the testis homogenates. The histology of the testes was analyzed with hematoxylin / PAS. ANOVA and Bonferroni were performed (significant differences at p <0.05). MS was confirmed by body weight gain, a decrease in waist circumference, blood glucose (mg/dL) (221.6±13.3 vs 143.0±13.0) and triglycerides (392.7±85.7 vs 86.9±21.9) and a decrease in HDL-cholesterol (25.0±1.1 vs 34.8±0.6). Qc normalized the biochemical parameters. Serum testosterone was similar in all groups. MS produced GSH depletion (nmol/mg prot.) (31.7±1.4 vs 45.1±0.5) and increased CAT activity (IU/mg prot.) (16.1±0.6 vs 9.8±1.6). Qc partially restored the tripeptide levels and normalized the enzyme activity. The area of the testicular sections and the thickness of the cell layer in the seminiferous tubules were lower in the MS group. At the end of the study, all groups presented complete spermatogenesis and the same concentration and motility of spermatozoa. The results suggest that MS produces OS and alters testicular tissue. Although complete spermatogenesis occurs during the development of MS, OS could be the mechanism by which fertility would be affected at longer times. Qc normalizes the testicular redox status and improves the general conditions of MS, which could prevent the development of male subfertility. El Síndrome Metabólico (SM) genera estrés oxidativo (EO) generalizado y, a nivel testicular, afecta la fertilidad masculina. La suplementación con Quercetina (Qc), podría mejorar esta condición. Objetivo: esclarecer los mecanismos por los cuales el SM afecta al tejido testicular y determinar si estas alteraciones son revertidas por el tratamiento con Qc. Ratas Wistar machos se dividieron en grupo control (C, n:5), C+Qc (n:4, 50mg/kg/día, vía oral, 15 días), SM (n:4, 10% de fructosa en el agua, 45 días) y SM+Qc (n:4, SM 30 días más 15 días con tratamiento de Qc). El tiempo del estudio fue de 45 días. Se midió peso corporal y circunferencia abdominal y en suero se determinó, glucosa, triglicéridos, colesterol, HDL-colesterol y testosterona. En muestras de epidídimo se determinó vitalidad, motilidad y número de espermatozoides. En testículo se cuantificó glutatión (GSH) y actividad de catalasa (CAT). La histología de los testículos se analizó con hematoxilina/PAS. Se realizó ANOVA y Bonferroni (diferencias significativas, p<0,05). El SM se confirmó por la ganancia de peso corporal (g) (127,0±6,8 vs 38,3±5,4;), perímetro de cintura (cm) (20,7±0,2 vs 16,4±0,2), glucemia (mg/dL) (221,6±13,3 vs 143,0±13,0), trigliceridemia (392,7±85,7 vs 86,9±21,9) y disminución de HDL-c (25,0±1,1 vs 34,8±0,6). Qc normalizó los parámetros bioquímicos. La testosterona sérica fue similar en todos los grupos. El SM produjo depleción de GSH (nmol/mg prot.) (31,7±1,4 vs 45,1±0,5) y aumento de CAT (UI/mg prot.) (16,1±0,6 vs 9,8±1,6) testicular. Qc restauró parcialmente los niveles del tripéptido y normalizó la actividad enzimática. El área de las secciones testiculares y el espesor de la capa celular en los túbulos seminíferos fueron menor en el grupo SM. En el tiempo de estudio, todos los grupos presentaron una espermatogénesis completa, igual concentración y motilidad de espermatozoides. Los resultados sugieren que el SM produce EO y altera el tejido testicular. A pesar que en el tiempo de desarrollo del SM se produce espermatogénesis completa, el EO podría ser el mecanismo por el cual a tiempos más prolongados se afectaría la fertilidad. Qc normaliza el estado redox testicular y mejora las condiciones generales del SM, lo que podría evitar el desarrollo de la subfertilidad masculina. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto https://revistas.unc.edu.ar/index.php/med/article/view/35053 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |