Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy

Abstract:  Parvovirus B19 (B19V) is a human pathogen prevalent worldwide, responsible for various diseases. If the primary infection occurs during pregnancy, it can cause placental and/or fetal pathologies (including anemia, congestive heart failure, hydrops and fetal death). The diagnosis...

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Autores principales: Colazo Salbetti , MB, Olivera , NL, Tenaglia , M, Hernández , G, Alfaro , J, Riberi , I, Isa , MB, Bertoldi , A, Boggio , G, Pedranti , M, Moreno , L, Adamo , MP
Formato: Artículo revista
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021
Materias:
parvovirus b19
serology
viremia
fetal hydrops
abortion
serología
hidropesía fetal
aborto
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/35025
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-35025
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
format Artículo revista
topic parvovirus b19
serology
viremia
fetal hydrops
abortion
parvovirus b19
serología
viremia
hidropesía fetal
aborto
spellingShingle parvovirus b19
serology
viremia
fetal hydrops
abortion
parvovirus b19
serología
viremia
hidropesía fetal
aborto
Colazo Salbetti , MB
Olivera , NL
Tenaglia , M
Hernández , G
Alfaro , J
Riberi , I
Isa , MB
Bertoldi , A
Boggio , G
Pedranti , M
Moreno , L
Adamo , MP
Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
topic_facet parvovirus b19
serology
viremia
fetal hydrops
abortion
parvovirus b19
serología
viremia
hidropesía fetal
aborto
author Colazo Salbetti , MB
Olivera , NL
Tenaglia , M
Hernández , G
Alfaro , J
Riberi , I
Isa , MB
Bertoldi , A
Boggio , G
Pedranti , M
Moreno , L
Adamo , MP
author_facet Colazo Salbetti , MB
Olivera , NL
Tenaglia , M
Hernández , G
Alfaro , J
Riberi , I
Isa , MB
Bertoldi , A
Boggio , G
Pedranti , M
Moreno , L
Adamo , MP
author_sort Colazo Salbetti , MB
title Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
title_short Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
title_full Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
title_fullStr Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
title_full_unstemmed Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy
title_sort immunological and virological markers for the diagnosis of human parvovirus b19 infection during pregnancy
description Abstract:  Parvovirus B19 (B19V) is a human pathogen prevalent worldwide, responsible for various diseases. If the primary infection occurs during pregnancy, it can cause placental and/or fetal pathologies (including anemia, congestive heart failure, hydrops and fetal death). The diagnosis in these cases is not standardized and there are limitations related to costs, type and timing of clinical samples, as well as laboratory techniques applied in each situation. Therefore, the aim of this study was to characterize the presence of B19V in suspected cases of congenital infection, determining specific immunological and virological markers useful for diagnosis. Retrospective, observational, analytical and cross-sectional design. Serum samples from pregnant women with maternal or fetal-neonatal clinical manifestations related to B19V infection stored in the biobank of the Clínica Universitaria Reina Fabiola (CURF) were selected according to the digital medical record (Jan 2018-Mar 2021). Infection was determined by viral DNA detection and serology. Protocol approved by the Institutional Committee of Ethics in Health Research of the CURF. Sixty-five samples were included (63 from pregnant women and 2 from newborns). We confirmed recent or ongoing acute infection in 8/65 (12.3%), all in pregnant women (8/63, 12.7%). Of these, 3/8 were IgM-positive, 5/8 DNA-positive (none with both markers) and 6/8 IgG-positive. A co-detection for the TORCH panel (Herpes simplex-2 IgM) was detected in only one patient. The clinical manifestations included: abortions 6/8(75%), fetal hydrops 3/8(37.5%), maternal anemia 2/8(25%), fetal cardiac alterations 2/8(25%), fetal anemia 1/8(12.5%), hepatosplenomegaly 1/8(12.5%), and polyhydramnios 1/8(12.5%). Of the abortions, 2/6(33.3%) were recurrent, 1/6(16.7%) developed hydrops, 1/6(16.7%) anemia and 1/6(16.7%) fetal heart abnormalities. One pregnant woman presented a rash in the arms (sign of infection). In conclusion, a high frequency of B19V is reported. B19V infection can be asymptomatic or present nonspecific manifestations during pregnancy, thus early suspicion/detection with a broad case definition is important. Determination of all specific markers is necessary for the diagnosis.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2021
url https://revistas.unc.edu.ar/index.php/med/article/view/35025
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first_indexed 2024-09-03T21:02:59Z
last_indexed 2024-09-03T21:02:59Z
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spelling I10-R327-article-350252024-04-15T16:19:09Z Immunological and virological markers for the diagnosis of human parvovirus B19 infection during pregnancy Marcadores inmunológicos y virológicos para el diagnóstico de la infección por parvovirus humano B19 durante la gestación Colazo Salbetti , MB Olivera , NL Tenaglia , M Hernández , G Alfaro , J Riberi , I Isa , MB Bertoldi , A Boggio , G Pedranti , M Moreno , L Adamo , MP parvovirus b19 serology viremia fetal hydrops abortion parvovirus b19 serología viremia hidropesía fetal aborto Abstract:  Parvovirus B19 (B19V) is a human pathogen prevalent worldwide, responsible for various diseases. If the primary infection occurs during pregnancy, it can cause placental and/or fetal pathologies (including anemia, congestive heart failure, hydrops and fetal death). The diagnosis in these cases is not standardized and there are limitations related to costs, type and timing of clinical samples, as well as laboratory techniques applied in each situation. Therefore, the aim of this study was to characterize the presence of B19V in suspected cases of congenital infection, determining specific immunological and virological markers useful for diagnosis. Retrospective, observational, analytical and cross-sectional design. Serum samples from pregnant women with maternal or fetal-neonatal clinical manifestations related to B19V infection stored in the biobank of the Clínica Universitaria Reina Fabiola (CURF) were selected according to the digital medical record (Jan 2018-Mar 2021). Infection was determined by viral DNA detection and serology. Protocol approved by the Institutional Committee of Ethics in Health Research of the CURF. Sixty-five samples were included (63 from pregnant women and 2 from newborns). We confirmed recent or ongoing acute infection in 8/65 (12.3%), all in pregnant women (8/63, 12.7%). Of these, 3/8 were IgM-positive, 5/8 DNA-positive (none with both markers) and 6/8 IgG-positive. A co-detection for the TORCH panel (Herpes simplex-2 IgM) was detected in only one patient. The clinical manifestations included: abortions 6/8(75%), fetal hydrops 3/8(37.5%), maternal anemia 2/8(25%), fetal cardiac alterations 2/8(25%), fetal anemia 1/8(12.5%), hepatosplenomegaly 1/8(12.5%), and polyhydramnios 1/8(12.5%). Of the abortions, 2/6(33.3%) were recurrent, 1/6(16.7%) developed hydrops, 1/6(16.7%) anemia and 1/6(16.7%) fetal heart abnormalities. One pregnant woman presented a rash in the arms (sign of infection). In conclusion, a high frequency of B19V is reported. B19V infection can be asymptomatic or present nonspecific manifestations during pregnancy, thus early suspicion/detection with a broad case definition is important. Determination of all specific markers is necessary for the diagnosis. Resumen:  El parvovirus B19 (B19V) es un patógeno humano de prevalencia mundial, responsable de diversas enfermedades. Cuando la primoinfección ocurre durante la gestación, puede ocasionar patologías placentarias o en el producto de la gestación (anemia, insuficiencia cardíaca congestiva, hidrops y muerte fetal). En estos casos el diagnóstico no está estandarizado y persisten limitaciones relacionadas a los costos, tipo y momento de toma de muestras clínicas, así como técnicas laboratoriales que se aplican en cada situación. Con este marco los objetivos fueron caracterizar la presencia de B19V en casos sospechosos de infección congénita y relevar marcadores inmunológicos y virológicos para su diagnóstico. Diseño retrospectivo, observacional, analítico y de corte transversal. Se recuperaron muestras de suero de embarazadas con manifestaciones clínicas maternas o feto-neonatales compatibles a infección por B19V del biobanco de la Clínica Universitaria Reina Fabiola (CURF), seleccionadas según la historia clínica digital (ene2018-mar2021). La infección se determinó por detección de ADN viral y serología. Protocolo aprobado por el Comité Institucional de Ética en Investigación en Salud de la CURF. Se incluyeron 65 muestras (63 de embarazadas y 2 de recién nacidos), confirmándose infección aguda en curso o reciente en 8/65(12,3%), todas del grupo de embarazadas (8/63, 12,7%). De ellas, 3/8 resultaron IgM-positivas, 5/8 ADN-positivas (ninguna con ambos marcadores concomitantes) y 6/8 IgG-positivas. Sólo un caso presentó co-detección con virus herpes simple-2(IgM-positivo); el resto de la pesquisa de agentes TORCH resultó negativa. Las manifestaciones clínicas incluyeron: abortos 6/8(75%), hidrops fetal 3/8(37,5%), anemia materna 2/8(25%), alteraciones cardíacas fetales 2/8(25%), anemia fetal 1/8(12,5%), hepatoesplenomegalia 1/8(12,5%), y polihidramnios 1/8(12,5%). De los 6 casos de abortos, 2/6(33,3%) fueron recurrentes, 1/6(16,7%) desarrolló hidrops, 1/6(16,7%) anemia y 1/6(16,7%) alteraciones cardíacas fetales. En un caso, la embarazada presentó exantema en extremidades (signo marcador de la infección). En conclusión, se detectó una alta frecuencia de B19V en los casos estudiados. Debido a que la infección por B19V puede ser asintomática o con manifestaciones inespecíficas durante el embarazo,  es importante la detección a través de la sospecha temprana con una definición de caso amplia. Es necesaria la determinación de todos los marcadores específicos a fin de arribar al diagnóstico. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/35025 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0

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