Prevalence of HHV-6 A and B in semen of men with impaired fertility

Abstract:  Male infertility is considered a multifactorial condition that sometimes have an unknown origin. Between all the possible influencing factors, male reproductive tract infections are considered a very common reasons for infertility. These infections can disrupt fertility by d...

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Autores principales: Biganzoli , P, Ferreyra, L, Olivera , C, Kiguen , X, Venezuela , F, Mosmann , J, Rivero , V, Cuffuni , C
Formato: Artículo revista
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021
Materias:
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Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/34949
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id I10-R327-article-34949
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
format Artículo revista
topic HHV-6A
HHV6-B
infertility
HHV-6A
HHV6-B
infertilidad
.
spellingShingle HHV-6A
HHV6-B
infertility
HHV-6A
HHV6-B
infertilidad
.
Biganzoli , P
Ferreyra, L
Olivera , C
Kiguen , X
Venezuela , F
Mosmann , J
Rivero , V
Cuffuni , C
Prevalence of HHV-6 A and B in semen of men with impaired fertility
topic_facet HHV-6A
HHV6-B
infertility
HHV-6A
HHV6-B
infertilidad
.
author Biganzoli , P
Ferreyra, L
Olivera , C
Kiguen , X
Venezuela , F
Mosmann , J
Rivero , V
Cuffuni , C
author_facet Biganzoli , P
Ferreyra, L
Olivera , C
Kiguen , X
Venezuela , F
Mosmann , J
Rivero , V
Cuffuni , C
author_sort Biganzoli , P
title Prevalence of HHV-6 A and B in semen of men with impaired fertility
title_short Prevalence of HHV-6 A and B in semen of men with impaired fertility
title_full Prevalence of HHV-6 A and B in semen of men with impaired fertility
title_fullStr Prevalence of HHV-6 A and B in semen of men with impaired fertility
title_full_unstemmed Prevalence of HHV-6 A and B in semen of men with impaired fertility
title_sort prevalence of hhv-6 a and b in semen of men with impaired fertility
description Abstract:  Male infertility is considered a multifactorial condition that sometimes have an unknown origin. Between all the possible influencing factors, male reproductive tract infections are considered a very common reasons for infertility. These infections can disrupt fertility by damaging spermiogenesis, altering sperm function or causing obstruction of the seminal ducts. The microorganisms most associated with this pathology are: Neisseria gonorrhoeae, Chlamydia trachomatis, Herpes simplex virus, Ureaplasma urealitycum, among others. In recent years, several studies have reported the presence in semen samples of human Herpesviruses 6 A and B (HHV-6A and B). These are two viruses cause persistent infections in a high percentage of the world’s population and are mainly associated with infantile roseola. HHV-6A and B infection are currently under study in the genitalia and with unknown consequences. The objective of this study was to estimate the prevalence of HHV-6A and B in the semen of infertile men in our region. We studied 100 semen samples from men with fertility alterations due to a nested PCR reaction for the detection of HHV-6A and HHV-6B genomic DNA. In addition, in 87 of the 100 samples the results were related to the presence of other infections: Human Papillomavirus (HPV), Chlamydia trachomatis, Herpes simplex virus 1 and 2, Ureaplasma urealitycum and Micoplasma hominis. Twelve samples were positive for HHV-6A and 13 for HHV-6B, resulting in prevalence of 12% (12/100) and 13% (13/100) respectively. Neither sample tested were positive for both viruses. HHV-6A or B were detected with some other pathogens studied as a co-infection, but in 9 of the 100 samples, we only identified HHV-6A or B. This samples were negative for the other pathogenic microorganisms. Conclusion: due to the considerable observed prevalence of HHV-6A and B, and the enough evidence that many samples presented these viruses as the only genital pathogens, these viruses deserve to be further studied and clarified their pathogenic roles in male infertility.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2021
url https://revistas.unc.edu.ar/index.php/med/article/view/34949
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spelling I10-R327-article-349492024-04-15T16:19:09Z Prevalence of HHV-6 A and B in semen of men with impaired fertility Prevalencia de HHV-6 A y B en semen de hombres con alteración de su fertilidad A Biganzoli , P Ferreyra, L Olivera , C Kiguen , X Venezuela , F Mosmann , J Rivero , V Cuffuni , C HHV-6A HHV6-B infertility HHV-6A HHV6-B infertilidad . Abstract:  Male infertility is considered a multifactorial condition that sometimes have an unknown origin. Between all the possible influencing factors, male reproductive tract infections are considered a very common reasons for infertility. These infections can disrupt fertility by damaging spermiogenesis, altering sperm function or causing obstruction of the seminal ducts. The microorganisms most associated with this pathology are: Neisseria gonorrhoeae, Chlamydia trachomatis, Herpes simplex virus, Ureaplasma urealitycum, among others. In recent years, several studies have reported the presence in semen samples of human Herpesviruses 6 A and B (HHV-6A and B). These are two viruses cause persistent infections in a high percentage of the world’s population and are mainly associated with infantile roseola. HHV-6A and B infection are currently under study in the genitalia and with unknown consequences. The objective of this study was to estimate the prevalence of HHV-6A and B in the semen of infertile men in our region. We studied 100 semen samples from men with fertility alterations due to a nested PCR reaction for the detection of HHV-6A and HHV-6B genomic DNA. In addition, in 87 of the 100 samples the results were related to the presence of other infections: Human Papillomavirus (HPV), Chlamydia trachomatis, Herpes simplex virus 1 and 2, Ureaplasma urealitycum and Micoplasma hominis. Twelve samples were positive for HHV-6A and 13 for HHV-6B, resulting in prevalence of 12% (12/100) and 13% (13/100) respectively. Neither sample tested were positive for both viruses. HHV-6A or B were detected with some other pathogens studied as a co-infection, but in 9 of the 100 samples, we only identified HHV-6A or B. This samples were negative for the other pathogenic microorganisms. Conclusion: due to the considerable observed prevalence of HHV-6A and B, and the enough evidence that many samples presented these viruses as the only genital pathogens, these viruses deserve to be further studied and clarified their pathogenic roles in male infertility. Resumen:  La infertilidad masculina es considerada una afección multifactorial que a veces es de origen desconocido. De todos los factores influyentes, las infecciones del tracto reproductor masculino son consideradas motivos muy frecuentes de infertilidad. Estas infecciones pueden alterar la fertilidad por deterioro de la espermiogénesis, alterando la función del espermatozoide o causando obstrucción de los conductos seminales. Algunos de los microorganismos mayormente asociados a esta patología son: Neisseria gonorrhoeae, Chlamydia trachomatis, Virus Herpes simplex, Ureaplasma urealitycum, entre otros. En los últimos años, varios estudios han reportado la presencia en muestras de semen de los Herpesvirus humanos 6 A y B (HHV-6A y B). Estos, son dos virus que hacen infecciones persistentes en un alto porcentaje de la población humana mundial y están asociados principalmente a la roséola infantil. La infección de los HHV-6A y B en los genitales es actualmente poco estudiada y de consecuencias desconocidas. El objetivo del presente trabajo fue estimar la prevalencia de HHV-6A y B en el semen de hombres infértiles de nuestra región. Se estudiaron 100 muestras de semen provenientes de hombres con alteraciones de fertilidad por una reacción de nested PCR para la detección del ADN genómico de HHV-6A y HHV-6B. Además, en 87 de las 100 muestras se relacionaron los resultados con la presencia de otras infecciones: Virus del Papiloma humano (HPV), Chlamydia trachomatis, Virus Herpes simplex 1 y 2, Ureaplasma urealitycum y Micoplasma hominis. Doce muestras resultaron positivas para HHV-6A y 13 para HHV-6B, resultando en prevalencias de 12 % (12/100) y 13 % (13/100) respectivamente. Ninguna muestra resultó positiva para ambos virus. Se detectaron también coinfecciones de HHV-6A o B con algunos de los otros patógenos estudiados. En 4 y 5 muestras diferentes; sólo se identificaron HHV-6A y HHV-6B, respectivamente; ya que fueron negativas para los otros microorganismos patógenos. Conclusión: debido a la considerable prevalencia observada de HHV-6A y B, y a que muchas muestras presentaron estos virus como únicos patógenos genitales, estos virus merecen ser más estudiados y aclarado sus roles patogénicos en la infertilidad masculina. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/34949 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0