Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma
Abstract: Oral squamous cell carcinoma (OSCC) is the eighth most prevalent cancer in the world, it has high morbidity and a low survival rate. The identification of biological markers allows increasing the sensitivity or specificity in the early detection or prognosis of malignancy in...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2021
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/34947 |
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I10-R327-article-34947 |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| format |
Artículo revista |
| topic |
oral squamous cell carcinoma connexin 43 Ki67 carcinoma oral de células escamosas conexina 43 Ki67 . |
| spellingShingle |
oral squamous cell carcinoma connexin 43 Ki67 carcinoma oral de células escamosas conexina 43 Ki67 . González-Segura , I Secchi, D Bologna-Molina , R Brunotto, M Centeno , V Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| topic_facet |
oral squamous cell carcinoma connexin 43 Ki67 carcinoma oral de células escamosas conexina 43 Ki67 . |
| author |
González-Segura , I Secchi, D Bologna-Molina , R Brunotto, M Centeno , V |
| author_facet |
González-Segura , I Secchi, D Bologna-Molina , R Brunotto, M Centeno , V |
| author_sort |
González-Segura , I |
| title |
Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| title_short |
Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| title_full |
Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| title_fullStr |
Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| title_full_unstemmed |
Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma |
| title_sort |
expression pattern of connexin 43 and ki67 in oral squamous cell carcinoma |
| description |
Abstract:
Oral squamous cell carcinoma (OSCC) is the eighth most prevalent cancer in the world, it has high morbidity and a low survival rate. The identification of biological markers allows increasing the sensitivity or specificity in the early detection or prognosis of malignancy in the population. The aim of this work was to determine the expression pattern of connexin 43 (Cx43) and Ki-67 in OSCC biopsies and control samples. Retrospective study of 24 samples of <18 years old, both genders, patients treated by spontaneous demand between 2014 and 2017 (approved by IRB Protocol No. 1379).OSCCs were diagnosed following the ICD10 classification code C00-C14. The controls were samples of normal gingival epithelium after dental extraction not associated with malignant or premalignant lesions.The expression levels of tumorigenic biomarkers were evaluated by immunohistochemical staining (IHQ). IHQ was analyzed in digital photomicrographs in 3 fields of 10-3 mm2using Image Pro Plus v13 software.Immunostainingwas classified according to the criteria of Tanaka et al. o y col. (2016) for Cx43 as low, medium and high expression, and positive-negative for Ki67 according to Schermaet al.(2017). The comparison between OSCC samples and controls was carried out using the Mann-Whitney test. The correlation was analyzed using the Spearman Coefficient (CS). A p-value <0.05 was set for statistical significance.Cx43 immunostaining was positive in 70% of control samples. Meanwhile, 90% of the cells presented low or null Cx43 immunostaining in OSCC samples (p = 0.0001). Ki67 labelling showed an elevated number of positive cells in CBCE samples concerning controls (p = 0.0007). Likely, in the process of malignant transformation, one of the first alterations in the epithelium of the buccal mucosa is the loss of Cx43 in the membrane, which modifies cell differentiation. In addition, the increase in cyclin Ki67 could be associated with the increase in cell proliferation. Both markers could be useful to identify the early stages of OSCC. (Work funded by FONCYT PICT2016–2358).
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| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2021 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/34947 |
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I10-R327-article-349472024-04-15T16:19:09Z Expression pattern of Connexin 43 and Ki67 in oral squamous cell carcinoma Patrón de expresión de Conexina 43 y Ki67 en carcinoma oral de células escamosas A González-Segura , I Secchi, D Bologna-Molina , R Brunotto, M Centeno , V oral squamous cell carcinoma connexin 43 Ki67 carcinoma oral de células escamosas conexina 43 Ki67 . Abstract: Oral squamous cell carcinoma (OSCC) is the eighth most prevalent cancer in the world, it has high morbidity and a low survival rate. The identification of biological markers allows increasing the sensitivity or specificity in the early detection or prognosis of malignancy in the population. The aim of this work was to determine the expression pattern of connexin 43 (Cx43) and Ki-67 in OSCC biopsies and control samples. Retrospective study of 24 samples of <18 years old, both genders, patients treated by spontaneous demand between 2014 and 2017 (approved by IRB Protocol No. 1379).OSCCs were diagnosed following the ICD10 classification code C00-C14. The controls were samples of normal gingival epithelium after dental extraction not associated with malignant or premalignant lesions.The expression levels of tumorigenic biomarkers were evaluated by immunohistochemical staining (IHQ). IHQ was analyzed in digital photomicrographs in 3 fields of 10-3 mm2using Image Pro Plus v13 software.Immunostainingwas classified according to the criteria of Tanaka et al. o y col. (2016) for Cx43 as low, medium and high expression, and positive-negative for Ki67 according to Schermaet al.(2017). The comparison between OSCC samples and controls was carried out using the Mann-Whitney test. The correlation was analyzed using the Spearman Coefficient (CS). A p-value <0.05 was set for statistical significance.Cx43 immunostaining was positive in 70% of control samples. Meanwhile, 90% of the cells presented low or null Cx43 immunostaining in OSCC samples (p = 0.0001). Ki67 labelling showed an elevated number of positive cells in CBCE samples concerning controls (p = 0.0007). Likely, in the process of malignant transformation, one of the first alterations in the epithelium of the buccal mucosa is the loss of Cx43 in the membrane, which modifies cell differentiation. In addition, the increase in cyclin Ki67 could be associated with the increase in cell proliferation. Both markers could be useful to identify the early stages of OSCC. (Work funded by FONCYT PICT2016–2358). Resumen: El carcinoma bucal a células escamosas (CBCE) es el octavo cáncer más prevalente en el mundo, presenta una elevada tasa de morbilidad y baja tasa de supervivencia. La identificación de marcadores biológicos permite aumentar la sensibilidad o especificidad de la detección precoz o pronóstico de malignidad en la población. El objetivo del trabajo fue determinar el patrón de expresión de conexina 43 (Cx43) y Ki-67 en biopsias de CBCEy controles. Estudio retrospectivo de 24 muestras de pacientes mayores de 18 años de ambos géneros atendidos por demanda espontánea entre el año 2014 a 2017 (aprobado por Protocolo IRB No. 1379). LosCBCEfueron diagnosticados siguiendo la clasificación CIE10 código C00-C14. Los controles fueron muestras de epitelio gingival normal post-extracción dentaria no asociadas a lesiones malignas o premalignas. Los niveles de expresión de los biomarcadores tumorigénicos se evaluaron mediante tinción inmunohistoquímica. La inmunomarcación se evaluó en microfotografías digitales en 3 campos de 10-3mm2utilizando el software Image Pro Plus v13. Se clasificó como expresión baja, media y alta según criterios de Tanaka el al (2016) para Cx43, y en positiva-negativa para Ki67 según Scherma et al (2017). La comparación entre muestras CBCE y controles se realizó mediante la Prueba Mann-Whitney. La correlación se analizó mediante el Coeficiente Spearman (CS). Se fijó un p<0,05 para significación estadística. En muestras controles se observó un 70% de inmunomarcación positiva Cx43; mientras que enmuestrasCBCEel 90% de las células presentó inmunomarcaciónbajaonula (p=0,0001).La marcación con Ki67 mostró un elevado número de células positivas en muestras de CBCE en relación a los controles (p=0,0007). Se observó una correlación positiva entre marcación positiva Ki67 y marcación nula o baja de Cx43 en membrana (CS=0,62).Posiblemente, en el proceso de transformación maligna, una de las primeras alteraciones en el epitelio de la mucosa bucal sea la pérdida de Cx43 de membrana, lo cual modifica la diferenciación celular. Además, el aumento de la ciclina Ki67 estaría asociado a un aumento de la proliferación celular. La detección de ambos marcadores podría ser útil para identificar etapas tempranas de CBCE. (Trabajo financiado por FONCYT PICT2016–2358). . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2021-10-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto texto texto https://revistas.unc.edu.ar/index.php/med/article/view/34947 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 78 No. Suplemento (2021): Suplemento JIC XXII Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 78 Núm. Suplemento (2021): Suplemento JIC XXII Revista da Faculdade de Ciências Médicas de Córdoba; v. 78 n. Suplemento (2021): Suplemento JIC XXII 1853-0605 0014-6722 http://creativecommons.org/licenses/by-nc/4.0 |