Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns
Most important burn treatment challenges are associated with control of infections and pain, and also with functional and aesthetic wound healing. Several experimental burn models have been performed in large mammals, however, small rodents such as rats, are the most commonly used species. The aim o...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25828 |
| Aporte de: |
| Sumario: | Most important burn treatment challenges are associated with control of infections and pain, and also with functional and aesthetic wound healing. Several experimental burn models have been performed in large mammals, however, small rodents such as rats, are the most commonly used species. The aim of this study was to evaluate the wound healing efficacy in a burn rat model treated with an anesthetic-antibiotic polymeric films (AAF), compared to the reference treatment.
A deep second degree burn model in male Wistar rats was used (CICUAL-UNLaR-5/18). Each anesthetized animal was injured in the dorsal region with a metallic device (1 cm ø, 90±2 ºC, 30sec). AAF treatment, control film (CF) or reference cream (RC: silver sulfadiazine, lidocaine and vitamin A) were applied once daily for 21 days (n=6). Control groups: untreated (UT) and not burned. Photographs and biopsies (H&E) were taken on days 0, 7, 14 and 21. Epidermal continuity and dermal organization were evaluated with scores according to Sanchez et al1.
Biopsies analysis showed that epidermis closure was reached in the order AAF> CF>UT>RC. Besides, burns treated with AAF presented complete dermis organization at day 21 and histological features similar to unburned control. These results could be related to the favorable moist environment provided by the components of AAF, that positively impact on the tissue recovery. In contrast, burns treated with RC did not complete the regeneration process by day 21 and even a regression was observed respect to day 14. Most of UT animals presented dense dermis and absence of skin annexes (day 21).
These findings suggest that the use of the AAF allowed a more rapid and better quality skin regeneration process with respect to the available reference treatment.
1Sanchez MF et al. Drug Deliv. and Transl. Res. Vol 8 (5): 1000-1013; 2018 |
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