Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns
Most important burn treatment challenges are associated with control of infections and pain, and also with functional and aesthetic wound healing. Several experimental burn models have been performed in large mammals, however, small rodents such as rats, are the most commonly used species. The aim o...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
|
| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25828 |
| Aporte de: |
| id |
I10-R327-article-25828 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
anesthetic-antibiotic polymeric films deep second degree burn wound healing membranas antibiótico-anestésicas quemaduras de segundo grado profundas regeneración de heridas |
| spellingShingle |
anesthetic-antibiotic polymeric films deep second degree burn wound healing membranas antibiótico-anestésicas quemaduras de segundo grado profundas regeneración de heridas Sanchez, MF Guzmán, ML Toledo, ME Laino, C Soria, EA Olivera, ME Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| topic_facet |
anesthetic-antibiotic polymeric films deep second degree burn wound healing membranas antibiótico-anestésicas quemaduras de segundo grado profundas regeneración de heridas |
| author |
Sanchez, MF Guzmán, ML Toledo, ME Laino, C Soria, EA Olivera, ME |
| author_facet |
Sanchez, MF Guzmán, ML Toledo, ME Laino, C Soria, EA Olivera, ME |
| author_sort |
Sanchez, MF |
| title |
Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| title_short |
Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| title_full |
Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| title_fullStr |
Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| title_full_unstemmed |
Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| title_sort |
preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns |
| description |
Most important burn treatment challenges are associated with control of infections and pain, and also with functional and aesthetic wound healing. Several experimental burn models have been performed in large mammals, however, small rodents such as rats, are the most commonly used species. The aim of this study was to evaluate the wound healing efficacy in a burn rat model treated with an anesthetic-antibiotic polymeric films (AAF), compared to the reference treatment.
A deep second degree burn model in male Wistar rats was used (CICUAL-UNLaR-5/18). Each anesthetized animal was injured in the dorsal region with a metallic device (1 cm ø, 90±2 ºC, 30sec). AAF treatment, control film (CF) or reference cream (RC: silver sulfadiazine, lidocaine and vitamin A) were applied once daily for 21 days (n=6). Control groups: untreated (UT) and not burned. Photographs and biopsies (H&E) were taken on days 0, 7, 14 and 21. Epidermal continuity and dermal organization were evaluated with scores according to Sanchez et al1.
Biopsies analysis showed that epidermis closure was reached in the order AAF> CF>UT>RC. Besides, burns treated with AAF presented complete dermis organization at day 21 and histological features similar to unburned control. These results could be related to the favorable moist environment provided by the components of AAF, that positively impact on the tissue recovery. In contrast, burns treated with RC did not complete the regeneration process by day 21 and even a regression was observed respect to day 14. Most of UT animals presented dense dermis and absence of skin annexes (day 21).
These findings suggest that the use of the AAF allowed a more rapid and better quality skin regeneration process with respect to the available reference treatment.
1Sanchez MF et al. Drug Deliv. and Transl. Res. Vol 8 (5): 1000-1013; 2018 |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25828 |
| work_keys_str_mv |
AT sanchezmf preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT guzmanml preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT toledome preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT lainoc preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT soriaea preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT oliverame preclinicalevaluationofnewantibioticanestheticmodifiedreleasesystemsforthetreatmentofdeepseconddegreeburns AT sanchezmf evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas AT guzmanml evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas AT toledome evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas AT lainoc evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas AT soriaea evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas AT oliverame evaluacionpreclinicadenuevossistemasantibioticoanestesicosdeliberacionmodificadaparaeltratamientodequemadurasdesegundogradoprofundas |
| first_indexed |
2024-09-03T21:01:09Z |
| last_indexed |
2024-09-03T21:01:09Z |
| _version_ |
1809210166994796544 |
| spelling |
I10-R327-article-258282024-08-27T18:26:26Z Preclinical evaluation of new antibiotic-anesthetic modified release systems for the treatment of deep second-degree burns Evaluación preclínica de nuevos sistemas antibiótico-anestésicos de liberación modificada para el tratamiento de quemaduras de segundo grado profundas Sanchez, MF Guzmán, ML Toledo, ME Laino, C Soria, EA Olivera, ME anesthetic-antibiotic polymeric films deep second degree burn wound healing membranas antibiótico-anestésicas quemaduras de segundo grado profundas regeneración de heridas Most important burn treatment challenges are associated with control of infections and pain, and also with functional and aesthetic wound healing. Several experimental burn models have been performed in large mammals, however, small rodents such as rats, are the most commonly used species. The aim of this study was to evaluate the wound healing efficacy in a burn rat model treated with an anesthetic-antibiotic polymeric films (AAF), compared to the reference treatment. A deep second degree burn model in male Wistar rats was used (CICUAL-UNLaR-5/18). Each anesthetized animal was injured in the dorsal region with a metallic device (1 cm ø, 90±2 ºC, 30sec). AAF treatment, control film (CF) or reference cream (RC: silver sulfadiazine, lidocaine and vitamin A) were applied once daily for 21 days (n=6). Control groups: untreated (UT) and not burned. Photographs and biopsies (H&E) were taken on days 0, 7, 14 and 21. Epidermal continuity and dermal organization were evaluated with scores according to Sanchez et al1. Biopsies analysis showed that epidermis closure was reached in the order AAF> CF>UT>RC. Besides, burns treated with AAF presented complete dermis organization at day 21 and histological features similar to unburned control. These results could be related to the favorable moist environment provided by the components of AAF, that positively impact on the tissue recovery. In contrast, burns treated with RC did not complete the regeneration process by day 21 and even a regression was observed respect to day 14. Most of UT animals presented dense dermis and absence of skin annexes (day 21). These findings suggest that the use of the AAF allowed a more rapid and better quality skin regeneration process with respect to the available reference treatment. 1Sanchez MF et al. Drug Deliv. and Transl. Res. Vol 8 (5): 1000-1013; 2018 El tratamiento de las quemaduras enfrenta desafíos asociados con el control de las infecciones, del dolor y la recuperación funcional y estética del tejido. Se han utilizado varios modelos experimentales de quemaduras en grandes mamíferos, como cerdos. Sin embargo, los roedores pequeños, como ratas o ratones, son las especies más utilizadas. El objetivo fue evaluar in vivo en ratas la eficacia de regeneración epitelial de quemaduras tratadas con membranas hidrocoloides antibióticas anestésicas (MAA) desarrolladas en nuestro grupo, en comparación con el tratamiento de referencia. Se utilizó un modelo de quemadura de segundo grado profundo en ratas macho Wistar. A cada animal anestesiado se le realizó una quemadura en la región dorsal con un dispositivo metálico (1 cm ø, 90±2 ºC, 30 segundos). Los tratamientos (n=6): MAA; membrana blanco (MB); referencia (R: crema de sulfadiazina de plata 1%, lidocaína 0,67% y vitamina A 248000 UI) se aplicaron una vez al día durante 21 días. Controles: no tratados (NT) y no quemado. Los días 0, 7, 14 y 21 se tomaron fotografías y biopsias (H/E). La continuidad epidérmica y la organización dérmica se evaluaron con scores de acuerdo con la metodología descripta en Sanchez et ali. El análisis de las biopsias mostró que el cierre de la epidermis se realizó en el orden MAA (completa día 14) > MB > NT (completa día 21) > R (incompleta día 21). Respecto de la dermis, las quemaduras tratadas con la MAA presentaron organización completa (día 21) y características histológicas similares al control no quemado. Esto se debe a que los componentes de la MAA generaron un entorno propicio para la regeneración, que impactaría positivamente en la recuperación funcional y estética del tejido. La mayoría de los animales NT presentaron dermis densas y ausencia de anexos cutáneos (día 21). Las quemaduras tratadas con R estaban en proceso de regeneración el día 21 e incluso se observó un retroceso respecto del día 14. La utilización de las MAA permitió la regeneración de la piel y fue superadora en tiempo y calidad respecto del tratamiento de referencia disponible. iSanchez MF et al. Drug Deliv. and Transl. Res. Vol 8 (5): 1000-1013; 2018 Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25828 Revista de la Facultad de Ciencias Médicas de Córdoba.; 2019: Suplemento JIC XX Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 10.31053/1853.0605.v76.nSuplemento spa https://revistas.unc.edu.ar/index.php/med/article/view/25828/27612 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |