Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring
Lipoic Acid (LA) is an essential cofactor of the mitochondrial respiratory enzymes from natural origin, and its anti-obesogenic effect its due to the AMPK suppression. Administration in the diet of LA have resulted in benefficial effects for liver functionality of ovariectomized fe...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25767 |
| Aporte de: |
| Sumario: | Lipoic Acid (LA) is an essential cofactor of the mitochondrial respiratory enzymes from natural origin, and its anti-obesogenic effect its due to the AMPK suppression. Administration in the diet of LA have resulted in benefficial effects for liver functionality of ovariectomized female rats. The aim of the present work was to evaluate the effect of maternal oral exposition with LA during the gestation and lactation on the liver histology and blood biochemical profile of ovariectomized female offspring.
Pregnant Wistar rats were fed ad-libitum with standard diet (food) + LA (0.4% wt/wt) from day 14 of gestation to day 20 of lactation (L, n=4) or food (C, n=4) as control. At 8 months of age, female offsprings born from LA fed rats (L) or controls (C) were randomly assigned to be ovariectomized (OV) or to receive a sham surgery (SH); resulting in 4 groups: LOV, LSH, COV and CSH (n=5/group). Food intake and body weight (BW) were measured weekly. Animals were euthanized after two months for samples’ collection. Liver histology was evaluated by using Hematoxilina/Eosina staining, and the blood metabolites were measured through spectrophotometry. Results were analyzed by ANOVA and were consider statistically significant at p<0.05.
No differences were observed between groups in daily food intake. Increased deposits of adipose tissue were observed in the histological sections of the COV group. Total fat tissue at the necropsy and plasmatic levels of triglycerides and total lipids were higher in the COV group (p<0.05) relative to the LOV, CSH y LSH groups ,with not differences between these 3 later groups.
Maternal intake of LA in the offspring reduce the alterations in the liver tissue and blood metabolites levels involved in the development of metabolic syndrome; which can be induced by estrogen deficiencies by ovariectomy. The effect of maternal intake of LA on the physiology of offspring could be caused by fetal programming phenomenon |
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