Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring
Lipoic Acid (LA) is an essential cofactor of the mitochondrial respiratory enzymes from natural origin, and its anti-obesogenic effect its due to the AMPK suppression. Administration in the diet of LA have resulted in benefficial effects for liver functionality of ovariectomized fe...
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| Autores principales: | , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2019
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/25767 |
| Aporte de: |
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I10-R327-article-25767 |
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| record_format |
ojs |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-327 |
| container_title_str |
Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
| topic |
lipoic acid metabolic syndrome fetal programming ácido lipoico síndrome metabólico programación fetal |
| spellingShingle |
lipoic acid metabolic syndrome fetal programming ácido lipoico síndrome metabólico programación fetal Elizondo, S Hernández Garrigo, P Lagares, C Moreira Espinoza, MJ Rabaglino, MB Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| topic_facet |
lipoic acid metabolic syndrome fetal programming ácido lipoico síndrome metabólico programación fetal |
| author |
Elizondo, S Hernández Garrigo, P Lagares, C Moreira Espinoza, MJ Rabaglino, MB |
| author_facet |
Elizondo, S Hernández Garrigo, P Lagares, C Moreira Espinoza, MJ Rabaglino, MB |
| author_sort |
Elizondo, S |
| title |
Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| title_short |
Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| title_full |
Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| title_fullStr |
Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| title_full_unstemmed |
Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| title_sort |
effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring |
| description |
Lipoic Acid (LA) is an essential cofactor of the mitochondrial respiratory enzymes from natural origin, and its anti-obesogenic effect its due to the AMPK suppression. Administration in the diet of LA have resulted in benefficial effects for liver functionality of ovariectomized female rats. The aim of the present work was to evaluate the effect of maternal oral exposition with LA during the gestation and lactation on the liver histology and blood biochemical profile of ovariectomized female offspring.
Pregnant Wistar rats were fed ad-libitum with standard diet (food) + LA (0.4% wt/wt) from day 14 of gestation to day 20 of lactation (L, n=4) or food (C, n=4) as control. At 8 months of age, female offsprings born from LA fed rats (L) or controls (C) were randomly assigned to be ovariectomized (OV) or to receive a sham surgery (SH); resulting in 4 groups: LOV, LSH, COV and CSH (n=5/group). Food intake and body weight (BW) were measured weekly. Animals were euthanized after two months for samples’ collection. Liver histology was evaluated by using Hematoxilina/Eosina staining, and the blood metabolites were measured through spectrophotometry. Results were analyzed by ANOVA and were consider statistically significant at p<0.05.
No differences were observed between groups in daily food intake. Increased deposits of adipose tissue were observed in the histological sections of the COV group. Total fat tissue at the necropsy and plasmatic levels of triglycerides and total lipids were higher in the COV group (p<0.05) relative to the LOV, CSH y LSH groups ,with not differences between these 3 later groups.
Maternal intake of LA in the offspring reduce the alterations in the liver tissue and blood metabolites levels involved in the development of metabolic syndrome; which can be induced by estrogen deficiencies by ovariectomy. The effect of maternal intake of LA on the physiology of offspring could be caused by fetal programming phenomenon |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2019 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/25767 |
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I10-R327-article-257672024-08-27T18:26:13Z Effects of maternal intake of α-lipoic acid on liver histology and biochemical profile of ovariectomized female offspring Efecto del consumo materno de ácido α-lipoico sobre la histología hepática y perfil bioquímico de las crías hembras ovariectomizadas Elizondo, S Hernández Garrigo, P Lagares, C Moreira Espinoza, MJ Rabaglino, MB lipoic acid metabolic syndrome fetal programming ácido lipoico síndrome metabólico programación fetal Lipoic Acid (LA) is an essential cofactor of the mitochondrial respiratory enzymes from natural origin, and its anti-obesogenic effect its due to the AMPK suppression. Administration in the diet of LA have resulted in benefficial effects for liver functionality of ovariectomized female rats. The aim of the present work was to evaluate the effect of maternal oral exposition with LA during the gestation and lactation on the liver histology and blood biochemical profile of ovariectomized female offspring. Pregnant Wistar rats were fed ad-libitum with standard diet (food) + LA (0.4% wt/wt) from day 14 of gestation to day 20 of lactation (L, n=4) or food (C, n=4) as control. At 8 months of age, female offsprings born from LA fed rats (L) or controls (C) were randomly assigned to be ovariectomized (OV) or to receive a sham surgery (SH); resulting in 4 groups: LOV, LSH, COV and CSH (n=5/group). Food intake and body weight (BW) were measured weekly. Animals were euthanized after two months for samples’ collection. Liver histology was evaluated by using Hematoxilina/Eosina staining, and the blood metabolites were measured through spectrophotometry. Results were analyzed by ANOVA and were consider statistically significant at p<0.05. No differences were observed between groups in daily food intake. Increased deposits of adipose tissue were observed in the histological sections of the COV group. Total fat tissue at the necropsy and plasmatic levels of triglycerides and total lipids were higher in the COV group (p<0.05) relative to the LOV, CSH y LSH groups ,with not differences between these 3 later groups. Maternal intake of LA in the offspring reduce the alterations in the liver tissue and blood metabolites levels involved in the development of metabolic syndrome; which can be induced by estrogen deficiencies by ovariectomy. The effect of maternal intake of LA on the physiology of offspring could be caused by fetal programming phenomenon El ácido α-lipoico(AL) es un cofactor esencial de origen natural de las enzimas respiratorias mitocondriales, y su efecto anti-obesogénico es debido a la supresión del AMPK. La administración en la dieta de AL ha resultado en efectos benéficos para la funcionalidad hepática de ratas hembras ovariectomizadas. El objetivo el presente trabajo fue evaluar el efecto de la exposición oral materna a AL durante la gestación y lactancia sobre la histología hepática y perfil bioquímico sanguíneo de las crías ovariectomizadas. Ratas Wistar preñadas fueron alimentadas ad-libitum con dietas estándar (alimento) + AL (0,4% p/p) desde los 14 días de gestación hasta los 21 días de lactancia (L, n=4) o alimento (C, n=4) como control. A las 8 meses de edad, las crías hembras nacidas de las ratas alimentadas con AL o controles fueron asignadas aleatoriamente para ser ovariectomizadas (OV) o para recibir una cirugía simulada (SH) resultando en 4 grupos: LOV, LSH, COV y CSH (n=5 por grupo). El consumo de alimento y el peso corporal fueron medidos semanalmente. Los animales fueron eutanasiados después de 2 meses, para la colección de las muestras. La histología hepática fue evaluada a través de la tinción con Hematoxilina/Eosina, y los metabolitos sanguíneos fueron cuantificados mediante espectrofotometría. Los resultados fueron analizados por ANOVA, y se consideraron estadísticamente significativo a un p<0,05. No se observó diferencias entre los grupos en el consumo diario de comida. Se observaron mayores depósitos de tejido adiposo en los cortes histológicos de hígado del grupo COV. El tejido de grasa total a la necropsia y los niveles plasmáticos de triglicéridos y lípidos totales fueron mayores en el grupo COV (p<0.05) relativo a los grupos LOV, CSH y LSH, sin diferencia entre estos tres grupos. La ingesta materna de AL disminuye, en las crías, las alteraciones en el tejido hepático y los niveles sanguíneos de metabolitos involucrados en el desarrollo de síndrome metabólico; los cuales pueden ser inducidos por la deficiencia de estrógenos inducida por ovariectomía. El efecto del consumo materno de AL sobre la fisiología de las crías es posiblemente causado por el fenómeno de programación fetal Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25767 Revista de la Facultad de Ciencias Médicas de Córdoba.; 2019: Suplemento JIC XX Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 10.31053/1853.0605.v76.nSuplemento spa https://revistas.unc.edu.ar/index.php/med/article/view/25767/27523 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |