Enterovirus detection in respiratory samples from the city of Córdoba

Acute Respiratory Infections (ARI) are defined as infections of the respiratory system, caused by viruses or bacteria, of evolution less than 15 days whose symptoms are cough, rhinorrhea, nasal obstruction, dysphonia or dyspnea, accompanied or not by fever. Among the most frequently found resp...

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Autores principales: López , R, Cámara, A, Adamo, P, Aguilar, J, Konigheim, B, Nates, S, Farias, A
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019
Materias:
enterovirus
ANF
Virus respiratorios
IRA
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/25750
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-25750
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic enterovirus
ANF
Virus respiratorios
IRA
enterovirus
ANF
Virus respiratorios
IRA
spellingShingle enterovirus
ANF
Virus respiratorios
IRA
enterovirus
ANF
Virus respiratorios
IRA
López , R
Cámara, A
Adamo, P
Aguilar, J
Konigheim, B
Nates, S
Farias, A
Enterovirus detection in respiratory samples from the city of Córdoba
topic_facet enterovirus
ANF
Virus respiratorios
IRA
enterovirus
ANF
Virus respiratorios
IRA
author López , R
Cámara, A
Adamo, P
Aguilar, J
Konigheim, B
Nates, S
Farias, A
author_facet López , R
Cámara, A
Adamo, P
Aguilar, J
Konigheim, B
Nates, S
Farias, A
author_sort López , R
title Enterovirus detection in respiratory samples from the city of Córdoba
title_short Enterovirus detection in respiratory samples from the city of Córdoba
title_full Enterovirus detection in respiratory samples from the city of Córdoba
title_fullStr Enterovirus detection in respiratory samples from the city of Córdoba
title_full_unstemmed Enterovirus detection in respiratory samples from the city of Córdoba
title_sort enterovirus detection in respiratory samples from the city of córdoba
description Acute Respiratory Infections (ARI) are defined as infections of the respiratory system, caused by viruses or bacteria, of evolution less than 15 days whose symptoms are cough, rhinorrhea, nasal obstruction, dysphonia or dyspnea, accompanied or not by fever. Among the most frequently found respiratory viruses are Respiratory Syncytial Virus (RSV), Bocavirus (BV), Adenovirus (AV), Influenza A and B (Flu), and Parainfluenza 1, 2 and 3 (PIV). From an outbreak of Enterovirus (EV) in 2014, causing respiratory cases of various varieties, EVs have taken a place in this scenario, although the prevalence of different serotypes is unknown. Objective. Detect the presence of EV of retrospective samples in cases of ARI of Córdoba. 138 samples of nasopharyngeal aspirate (ANF) from the years 2007, 2009, 2011, 2012, 2016 and 2017, previously tested for VSR, hMPV, Flu, PIV, AV, Rhinovirus and BV were randomly selected from the biobank of the Virology Institute. The presence of EV was determined by RT-nested PCR specific to the VP1 region. CIES Oulton approval 12/20/2017. EV was found in 13% (18/138) of the samples; 62% (11/18) was recorded in autumn followed by 38% (7/18) in winter. There was no detection of EV in the spring and summer samples. The highest number of cases, 13/18 (72%), was registered in children under one year of male sex. Of the total Positive EV, 83% corresponded to monoinfection with the virus. The years with the highest to lowest detection percentage were 2016,2017 and 2007 respectively. It was not possible to identify the circulating serotypes. The results indicate the presence of EV in ANF samples of cases of ARF during autumn and winter, mainly under one year. The detection frequency was similar to that of other circulating respiratory viruses. Due to the existence of coinfections, in order to make a correct differential diagnosis, it is important to take into account the epidemiology of EVs and their serotypes in cases of ARI.  
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2019
url https://revistas.unc.edu.ar/index.php/med/article/view/25750
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spelling I10-R327-article-257502024-08-27T18:26:11Z Enterovirus detection in respiratory samples from the city of Córdoba Detección de Enterovirus en muestras respiratorias de la ciudad de Córdoba López , R Cámara, A Adamo, P Aguilar, J Konigheim, B Nates, S Farias, A enterovirus ANF Virus respiratorios IRA enterovirus ANF Virus respiratorios IRA Acute Respiratory Infections (ARI) are defined as infections of the respiratory system, caused by viruses or bacteria, of evolution less than 15 days whose symptoms are cough, rhinorrhea, nasal obstruction, dysphonia or dyspnea, accompanied or not by fever. Among the most frequently found respiratory viruses are Respiratory Syncytial Virus (RSV), Bocavirus (BV), Adenovirus (AV), Influenza A and B (Flu), and Parainfluenza 1, 2 and 3 (PIV). From an outbreak of Enterovirus (EV) in 2014, causing respiratory cases of various varieties, EVs have taken a place in this scenario, although the prevalence of different serotypes is unknown. Objective. Detect the presence of EV of retrospective samples in cases of ARI of Córdoba. 138 samples of nasopharyngeal aspirate (ANF) from the years 2007, 2009, 2011, 2012, 2016 and 2017, previously tested for VSR, hMPV, Flu, PIV, AV, Rhinovirus and BV were randomly selected from the biobank of the Virology Institute. The presence of EV was determined by RT-nested PCR specific to the VP1 region. CIES Oulton approval 12/20/2017. EV was found in 13% (18/138) of the samples; 62% (11/18) was recorded in autumn followed by 38% (7/18) in winter. There was no detection of EV in the spring and summer samples. The highest number of cases, 13/18 (72%), was registered in children under one year of male sex. Of the total Positive EV, 83% corresponded to monoinfection with the virus. The years with the highest to lowest detection percentage were 2016,2017 and 2007 respectively. It was not possible to identify the circulating serotypes. The results indicate the presence of EV in ANF samples of cases of ARF during autumn and winter, mainly under one year. The detection frequency was similar to that of other circulating respiratory viruses. Due to the existence of coinfections, in order to make a correct differential diagnosis, it is important to take into account the epidemiology of EVs and their serotypes in cases of ARI.   Las Infecciones Respiratorias Agudas (IRA) se definen como infecciones del aparato respiratorio, causadas por virus o bacterias, de evolución menor a 15 días cuyos síntomas son tos, rinorrea, obstrucción nasal, disfonía o disnea, acompañados o no de fiebre. Entre los virus respiratorios más frecuentemente hallados se encuentran el Virus Sincicial Respiratorio (VSR), Bocavirus (BV), Adenovirus (AV), Influenza A y B (Flu), y Parainfluenza 1, 2 y 3 (PIV). A partir, de un brote de Enterovirus (EV) en 2014, causando cuadros respiratorios de diversa variedad, los EV han tomado un lugar en este escenario, aunque no se conoce la prevalencia de los diferentes serotipos. Objetivo. Detectar la presencia de EV de muestras retrospectivas en casos de IRA de Córdoba. Se seleccionaron aleatoriamente del biobanco del Instituto de Virología  138 muestras de aspirado nasofaringeo (ANF) de los años 2007, 2009, 2011, 2012, 2016 y 2017, previamente testeados  para VSR, hMPV, Flu, PIV, AV, Rinovirus y BV. Se determinó la presencia de EV mediante RT-nested PCR específica de la región VP1. Aprobación CIES Oulton 20/12/2017. Se halló EV en el 13% (18/138) de las muestras;  el 62% (11/18) se registro  en otoño seguido de un 38% (7/18) en invierno. No hubo detección de EV en las muestras de  primavera y verano.  El mayor número de casos, 13/18 (72%), se registro en menores de un año de sexo masculino.  Del total de EV Positivo, el 83% correspondio a monoinfeccion por el virus. Los años de mayor a menor porcentaje de detección fueron 2016,2017 y 2007 respectivamente. No se logro identificar los serotipos circulantes. Los resultados indican la presencia de EV en muestras de ANF de casos de IRA durante otoño e invierno, principalmente  menores de un año. La frecuencia de detección fue similar a la de otros virus respiratorios circulantes. Debido a la existencia de coinfecciones,  a fin de hacer un correcto diagnostico diferencial, es importante tener en cuenta la epidemiología de los EV y sus serotipos en los casos de IRA . Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2019-10-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/25750 Revista de la Facultad de Ciencias Médicas de Córdoba.; 2019: Suplemento JIC XX Revista de la Facultad de Ciencias Médicas de Córdoba; 2019: Suplemento JIC XX Revista da Faculdade de Ciências Médicas de Córdoba; 2019: Suplemento JIC XX 1853-0605 0014-6722 10.31053/1853.0605.v76.nSuplemento spa https://revistas.unc.edu.ar/index.php/med/article/view/25750/27427 Derechos de autor 2019 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0

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