Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina
Introduction: The Norovirus (NoV) constitute a genus within the viral family Caliciviridae, being the main cause of outbreaks of food origin among humans. Fresh vegetables are susceptible to being contaminated with these pathogens during their cultivation, harvest, transport, processing and handling...
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| Formato: | Artículo revista |
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Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2020
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| Materias: | |
| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/24070 |
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I10-R327-article-24070 |
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Universidad Nacional de Córdoba |
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I-10 |
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R-327 |
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Revista de la Facultad de Ciencias Médicas de Córdoba |
| language |
Español |
| format |
Artículo revista |
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norovirus vegetables food sprout norovirus vegetales alimentos brote norovírus vegetais alimentos surto |
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norovirus vegetables food sprout norovirus vegetales alimentos brote norovírus vegetais alimentos surto Coluccini, María Laura Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| topic_facet |
norovirus vegetables food sprout norovirus vegetales alimentos brote norovírus vegetais alimentos surto |
| author |
Coluccini, María Laura |
| author_facet |
Coluccini, María Laura |
| author_sort |
Coluccini, María Laura |
| title |
Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| title_short |
Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| title_full |
Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| title_fullStr |
Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| title_full_unstemmed |
Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina |
| title_sort |
leafy vegetables as potential transmitters of norovirus in cordoba city, argentina |
| description |
Introduction: The Norovirus (NoV) constitute a genus within the viral family Caliciviridae, being the main cause of outbreaks of food origin among humans. Fresh vegetables are susceptible to being contaminated with these pathogens during their cultivation, harvest, transport, processing and handling. So it was intended to determine the frequency of detection of NoV in plant samples of leaves of the City of Córdoba, and adapt a method of viral concentration with polyethylene glycol for the recovery of viral particles from the surface of vegetables and characterize the genogroups of NoV detected.
Methods: 19 samples of leafy vegetables were taken between June and December 2012. A viral concentration technique previously validated in the laboratory was applied (elution and precipitation with polyethylene glycol). The viral RNA was extracted to the concentrates of the samples using Trizol and precipitation with isopropyl alcohol. The nucleic acid was amplified by Rt-PCR with specific primers to identify genogroups I (GI) and II (GII). The products of the amplification were revealed by polyacrylamide gel electrophoresis and silver staining.
Results: We found 57.89% positive samples. Ten of the detected strains belonged to genogroup I (GI) and one to genogroup II (GII). They were identified throughout the study period, particularly during the months of August, September and November.
Conclusion: These pathogens were detected with a prevalence of 57.89%. The strains belonged mainly to the GI, representing a potential risk for the population. |
| publisher |
Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2020 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/24070 |
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2024-09-03T21:00:38Z |
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2024-09-03T21:00:38Z |
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1809210134489989120 |
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I10-R327-article-240702024-08-27T18:24:57Z Leafy vegetables as potential transmitters of Norovirus in Cordoba city, Argentina Vegetales de hojas como potenciales transmisores de Norovirus en la ciudad de Córdoba, Argentina Vegetais de folha como potenciais transmissores de Norovirus da cidade de Córdoba, Argentina Coluccini, María Laura norovirus vegetables food sprout norovirus vegetales alimentos brote norovírus vegetais alimentos surto Introduction: The Norovirus (NoV) constitute a genus within the viral family Caliciviridae, being the main cause of outbreaks of food origin among humans. Fresh vegetables are susceptible to being contaminated with these pathogens during their cultivation, harvest, transport, processing and handling. So it was intended to determine the frequency of detection of NoV in plant samples of leaves of the City of Córdoba, and adapt a method of viral concentration with polyethylene glycol for the recovery of viral particles from the surface of vegetables and characterize the genogroups of NoV detected. Methods: 19 samples of leafy vegetables were taken between June and December 2012. A viral concentration technique previously validated in the laboratory was applied (elution and precipitation with polyethylene glycol). The viral RNA was extracted to the concentrates of the samples using Trizol and precipitation with isopropyl alcohol. The nucleic acid was amplified by Rt-PCR with specific primers to identify genogroups I (GI) and II (GII). The products of the amplification were revealed by polyacrylamide gel electrophoresis and silver staining. Results: We found 57.89% positive samples. Ten of the detected strains belonged to genogroup I (GI) and one to genogroup II (GII). They were identified throughout the study period, particularly during the months of August, September and November. Conclusion: These pathogens were detected with a prevalence of 57.89%. The strains belonged mainly to the GI, representing a potential risk for the population. Introducción: Los Norovirus (NoV) constituyen un género dentro de la familia viral Caliciviridae, siendo el principal causante de brotes de origen alimentario entre humanos. Los vegetales frescos son susceptibles de ser contaminados con estos patógenos durante su cultivo, cosecha, transporte, procesamiento y manipulación. Por lo que se pretendió determinar la frecuencia de detección de NoV en muestras vegetales de hojas de la Ciudad de Córdoba, y adaptar un método de concentración viral con polietilenglicol para la recuperación de partículas virales de la superficie de vegetales y caracterizar los genogrupos de NoV detectados. Métodos: Se tomaron 19 muestras de vegetales de hoja entre junio y diciembre de 2012. Se aplicó una técnica de concentración viral validada previamente en el laboratorio (elución y precipitación con polietilenglicol). A los concentrados de las muestras se les extrajo el ARN viral utilizando Trizol y precipitación con alcohol isopropílico. Se amplificó el ácido nucleico por Rt-PCR con primers específicos para identificar genogrupos I (GI) y II (GII). Los productos de la amplificacion fueron revelados por electroforesis en geles de poliacrilamida y tinción argéntica. Resultados: Se encontraron 57,89% muestras positivas. Diez de las de cepas detectadas pertenecieron al genogrupo I (GI) y una al genogrupo II (GII). Se identificaron a lo largo de todo el periodo estudio, particularmente durante los meses de agosto, septiembre y noviembre. Conclusión: Se detectó a estos patógenos con una prevalencia de 57,89 %. Las cepas pertenecieron mayoritariamente al GI, representando un riesgo potencial para la población. Introdução: Os Norovírus (NoV) constituem um gênero dentro da família viral Caliciviridae, sendo a principal causa de surtos de origem alimentar nos humanos. Os vegetais frescos são susceptíveis a serem contaminados com estes patógenos durante seu cultivo, colheita, transporte, processamento e manipulação. O objetivo foi determinar a frequência de detecção de NoV em amostras de vegetais de folha da cidade de Córdoba, adaptando um método de concentração viral com polietilenoglicol para a recuperação de partículas virais da superfície de vegetais e caracterizar os genogrupos de NoV detectados. Métodos: Colheram-se dezenove amostras de vegetais de folha entre junho e dezembro de 2012. Aplicou-se uma técnica de concentração viral validada com antecedência no laboratório (eluição e precipitação com polietilenoglicol). Extraiu-se o RNA viral com Trizol e precipitação com álcool isopropílico dos concentrados das amostras. Amplificou-se o ácido nucleico por RT-PCR com primers específicos para identificar genogrupos I (GI) e II (GII). Os produtos da amplificação revelaram-se por electroforese em géis de poliacrilamida e tinção argírica. Resultados: Encontraram-se 57.89% de amostras positivas. Dez cepas pertenceram ao genogrupo I (GI) e uma ao genogrupo GII (GII). Identificaram-se ao longo de todo o período de estudo; especialmente durante os meses de agosto, setembro e novembro. Conclusão: A prevalência deste patógeno foi de 57.89%. A maioria das cepas pertenceu ao GI, representando um risco potencial para a população. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2020-03-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/med/article/view/24070 10.31053/1853.0605.v77.n1.24070 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 77 No. 1 (2020); 15-18 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 77 Núm. 1 (2020); 15-18 Revista da Faculdade de Ciências Médicas de Córdoba; v. 77 n. 1 (2020); 15-18 1853-0605 0014-6722 10.31053/1853.0605.v77.n1 spa https://revistas.unc.edu.ar/index.php/med/article/view/24070/29225 https://revistas.unc.edu.ar/index.php/med/article/view/24070/29246 Derechos de autor 2020 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0 |