Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte

The contact area between a T cell and antigen-presenting cell is known as “immunological synapse” and the multiple interactions that occur leading to a “signal” for T cell activation. However, an immunological synapse can occur in different circumstances, for a variety of functions. The phenomenon o...

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Detalles Bibliográficos
Autores principales: Novak, Ivón T. C., Orquera, Abel D.
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2012
Materias:
anticoagulantes
sinapsis inmune
interacción celular
linfocitos-macrófagos
rosetas
anticoagulants
immunological synapses
cell interaction
lymphocytes
macrophagesrosettes
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/21355
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-21355
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Español
format Artículo revista
topic anticoagulantes
sinapsis inmune
interacción celular
linfocitos-macrófagos
rosetas
anticoagulants
immunological synapses
cell interaction
lymphocytes
macrophagesrosettes
spellingShingle anticoagulantes
sinapsis inmune
interacción celular
linfocitos-macrófagos
rosetas
anticoagulants
immunological synapses
cell interaction
lymphocytes
macrophagesrosettes
Novak, Ivón T. C.
Orquera, Abel D.
Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
topic_facet anticoagulantes
sinapsis inmune
interacción celular
linfocitos-macrófagos
rosetas
anticoagulants
immunological synapses
cell interaction
lymphocytes
macrophagesrosettes
author Novak, Ivón T. C.
Orquera, Abel D.
author_facet Novak, Ivón T. C.
Orquera, Abel D.
author_sort Novak, Ivón T. C.
title Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
title_short Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
title_full Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
title_fullStr Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
title_full_unstemmed Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
title_sort effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte
description The contact area between a T cell and antigen-presenting cell is known as “immunological synapse” and the multiple interactions that occur leading to a “signal” for T cell activation. However, an immunological synapse can occur in different circumstances, for a variety of functions. The phenomenon of multiple immunological synapses on macrophage-lymphocyte rosette (MLR) (2, 3) refers to cellular association between human blood monocyte-derived macrophages and lymphocytes from autologous cultures total leukocytes extracted from the blood, which bind selectively forming rosettes with central macrophage and lymphocytes adhered. Inhibitors of antigen processing and presentation and monoclonal antibodies anti-Major Histocompatibility Complex Class II preclude the MLR phenomenon.Objective: As the MLR phenomenon was originally described using heparin, we studied the use of alternative anticoagulants. Materials and methods: human blood samples, anticoagulated with EDTA (ethylenediaminetetraacetic acid) (n = 10) Sodium citrate (n = 10) and controls with heparin (n = 10) healthy samples. Autologous cultures in medium TC199 (Sigma, St. Louis, MO). Culture sampleswere analysed at 72 h and 96 h. Results: In all heparin cases were observed MLRs, in sodium citrate cases, there was no viable leukocytes in cultures, and in cases with EDTA, leukocytes were observed, but the MLR phenomenon did not occur, there was no immune synapse. Conclusions: These results could be due to the chelating action of Ca2 + from the EDTA and sodium citrate as the inhibition of the leucoaccumulation of F-actin by EDTA, which affect the immune synapse in MLR phenomenon.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2012
url https://revistas.unc.edu.ar/index.php/med/article/view/21355
work_keys_str_mv AT novakivontc effectsofanticoagulantsontheimmunologicalsynapsesofhumanautologousrosettesbetweenmacrophageandlymphocyte
AT orqueraabeld effectsofanticoagulantsontheimmunologicalsynapsesofhumanautologousrosettesbetweenmacrophageandlymphocyte
AT novakivontc efectosdeanticoagulantessobrelassinapsisinmunologicasderosetasmacrofagolinfocitariasautologashumanas
AT orqueraabeld efectosdeanticoagulantessobrelassinapsisinmunologicasderosetasmacrofagolinfocitariasautologashumanas
first_indexed 2024-09-03T20:59:53Z
last_indexed 2024-09-03T20:59:53Z
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spelling I10-R327-article-213552024-08-27T18:23:06Z Effects of anticoagulants on the immunological synapses of human autologous rosettes between macrophage and lymphocyte Efectos de anticoagulantes sobre las sinapsis inmunologicas de rosetas macrofago-linfocitarias autologas humanas Novak, Ivón T. C. Orquera, Abel D. anticoagulantes sinapsis inmune interacción celular linfocitos-macrófagos rosetas anticoagulants immunological synapses cell interaction lymphocytes macrophagesrosettes The contact area between a T cell and antigen-presenting cell is known as “immunological synapse” and the multiple interactions that occur leading to a “signal” for T cell activation. However, an immunological synapse can occur in different circumstances, for a variety of functions. The phenomenon of multiple immunological synapses on macrophage-lymphocyte rosette (MLR) (2, 3) refers to cellular association between human blood monocyte-derived macrophages and lymphocytes from autologous cultures total leukocytes extracted from the blood, which bind selectively forming rosettes with central macrophage and lymphocytes adhered. Inhibitors of antigen processing and presentation and monoclonal antibodies anti-Major Histocompatibility Complex Class II preclude the MLR phenomenon.Objective: As the MLR phenomenon was originally described using heparin, we studied the use of alternative anticoagulants. Materials and methods: human blood samples, anticoagulated with EDTA (ethylenediaminetetraacetic acid) (n = 10) Sodium citrate (n = 10) and controls with heparin (n = 10) healthy samples. Autologous cultures in medium TC199 (Sigma, St. Louis, MO). Culture sampleswere analysed at 72 h and 96 h. Results: In all heparin cases were observed MLRs, in sodium citrate cases, there was no viable leukocytes in cultures, and in cases with EDTA, leukocytes were observed, but the MLR phenomenon did not occur, there was no immune synapse. Conclusions: These results could be due to the chelating action of Ca2 + from the EDTA and sodium citrate as the inhibition of the leucoaccumulation of F-actin by EDTA, which affect the immune synapse in MLR phenomenon. El área de contacto entre una célula T y una célula presentadora de antígenos es conocida como “sinapsis inmunológica” y las múltiples interacciones que ocurren conducen a una “señalización” para la activación de la célula T. Sin embargo, una sinapsis inmunológica puede ocurrir en diferentes circunstancias, para una variedad de funciones. El fenómeno de múltiples sinapsis inmunológicas de la roseta macrofago-linfocitaria (RML) (2, 3) se refiere a asociaciones celulares entre macrófagos derivados de monocitos y linfocitos autólogos humanos, a partir de cultivos de leucocitos totales extraídos de la sangre, que se unen selectivamente. El fenómeno RML es impedido por inhibidores del procesamiento y presentación antigénica y anticuerpos monoclonales anti-CMH Clase II.Objetivos: evaluar el uso de anticoagulantes alternativos para el estudio de sinapsis inmunes.Materiales y métodos: muestras de sangre humana sana, anticoagulada con EDTA (ethylenediaminetetraacétic acid) (n=10); con citrato de Sodio (n=10) y con heparina (n=10). Cultivos autólogos en medio TC199 (SIGMA, St. Louis, MO). Muestras a 72 h y 96 h. Resultados: en los casos con heparina se observaron RMLs; con citrato de Sodio, no se observaron leucocitos viables en cultivos; y con EDTA, se observaron leucocitos, pero no hubo sinapsis inmunes.Discusión: Estos resultados podrían deberse tanto a las acciones quelantes de Ca2+ del EDTA y del citrato de Sodio, como a la inhibición de la acumulación de F-actina por EDTA, que afectarían las sinapsis inmunes en el fenómeno RML.Conclusiones: La heparina es el anticoagulante de preferencia para el estudio de sinapsis inmunológicas. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2012-03-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/21355 10.31053/1853.0605.v69.n1.21355 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 69 No. 1 (2012); 20-24 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 69 Núm. 1 (2012); 20-24 Revista da Faculdade de Ciências Médicas de Córdoba; v. 69 n. 1 (2012); 20-24 1853-0605 0014-6722 10.31053/1853.0605.v69.n1 spa https://revistas.unc.edu.ar/index.php/med/article/view/21355/20872 Derechos de autor 2012 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0

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