Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children

Background: Parasitic infections by Trypanosoma cruzi (T. cruzi) are frequent in children from endemic areas. Specific therapies have been successfully used in pediatric populations to treat this disease. T. cruzi diagnosis should be optimized and become available for any clinical environment.Object...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sanchez, Ariel German, Alvarellos, Emilio, Kohout, Isolda, Rodriguez Schulz, Diego, Cordeiro, Enrique, Caeiro, Juan Pablo, Alvarellos, Teresita
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2016
Materias:
T. cruzi
PCR
dried blood spot
Acceso en línea:https://revistas.unc.edu.ar/index.php/med/article/view/14476
Aporte de:
Revista de la Facultad de Ciencias Médicas de Córdoba de Universidad Nacional de Córdoba
id I10-R327-article-14476
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-327
container_title_str Revista de la Facultad de Ciencias Médicas de Córdoba
language Inglés
format Artículo revista
topic T. cruzi
PCR
dried blood spot
spellingShingle T. cruzi
PCR
dried blood spot
Sanchez, Ariel German
Alvarellos, Emilio
Kohout, Isolda
Rodriguez Schulz, Diego
Cordeiro, Enrique
Caeiro, Juan Pablo
Alvarellos, Teresita
Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
topic_facet T. cruzi
PCR
dried blood spot
author Sanchez, Ariel German
Alvarellos, Emilio
Kohout, Isolda
Rodriguez Schulz, Diego
Cordeiro, Enrique
Caeiro, Juan Pablo
Alvarellos, Teresita
author_facet Sanchez, Ariel German
Alvarellos, Emilio
Kohout, Isolda
Rodriguez Schulz, Diego
Cordeiro, Enrique
Caeiro, Juan Pablo
Alvarellos, Teresita
author_sort Sanchez, Ariel German
title Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
title_short Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
title_full Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
title_fullStr Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
title_full_unstemmed Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children
title_sort detection of trypanosoma cruzi and treatment monitoring by pcr from dried blood spot samples in children
description Background: Parasitic infections by Trypanosoma cruzi (T. cruzi) are frequent in children from endemic areas. Specific therapies have been successfully used in pediatric populations to treat this disease. T. cruzi diagnosis should be optimized and become available for any clinical environment.Objective: To study T. cruzi prevalence in children from an area of active transmission and carry out a post-treatment follow-up. To verify the feasibility of detecting DNA ofT. cruzi from dried blood spot.Methods: We analyzed presence of T. cruzi in 78 Aboriginal children (Toba community) that attended to a rural school of Chaco province, Argentina. Serum and whole blood (dried blood spot) were assessed by means of serological techniques and PCR. Positive children received Benznidazole. Diagnosis and post treatment follow-up of T. cruzi infection were performed.Results: The serology assay showed infection in 34 of 78 (43.5%) children studied; PCR was positive in 5/34, displaying parasitemia. Serology remained positive in 28/28 children 120 days post-treatment, while PCR was positive in 18/28 (6/34 children were lost in follow-up). No adverse effects during the treatment were reported.Conclusions: We were able to establish T. cruzi prevalence in the studied population and also to prove the usefulness of dried blood spot for T. cruzi detection using PCR in isolated areas. This method allowed us to verify early treatment failure. Possible causes of this failure are discussed below.
publisher Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
publishDate 2016
url https://revistas.unc.edu.ar/index.php/med/article/view/14476
work_keys_str_mv AT sanchezarielgerman detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT alvarellosemilio detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT kohoutisolda detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT rodriguezschulzdiego detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT cordeiroenrique detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT caeirojuanpablo detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
AT alvarellosteresita detectionoftrypanosomacruziandtreatmentmonitoringbypcrfromdriedbloodspotsamplesinchildren
first_indexed 2024-09-03T20:58:15Z
last_indexed 2024-09-03T20:58:15Z
_version_ 1809209984572981248
spelling I10-R327-article-144762024-08-27T18:20:08Z Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children Detection of Trypanosoma cruzi and Treatment Monitoring by PCR from Dried Blood Spot Samples in Children Sanchez, Ariel German Alvarellos, Emilio Kohout, Isolda Rodriguez Schulz, Diego Cordeiro, Enrique Caeiro, Juan Pablo Alvarellos, Teresita T. cruzi PCR dried blood spot Background: Parasitic infections by Trypanosoma cruzi (T. cruzi) are frequent in children from endemic areas. Specific therapies have been successfully used in pediatric populations to treat this disease. T. cruzi diagnosis should be optimized and become available for any clinical environment.Objective: To study T. cruzi prevalence in children from an area of active transmission and carry out a post-treatment follow-up. To verify the feasibility of detecting DNA ofT. cruzi from dried blood spot.Methods: We analyzed presence of T. cruzi in 78 Aboriginal children (Toba community) that attended to a rural school of Chaco province, Argentina. Serum and whole blood (dried blood spot) were assessed by means of serological techniques and PCR. Positive children received Benznidazole. Diagnosis and post treatment follow-up of T. cruzi infection were performed.Results: The serology assay showed infection in 34 of 78 (43.5%) children studied; PCR was positive in 5/34, displaying parasitemia. Serology remained positive in 28/28 children 120 days post-treatment, while PCR was positive in 18/28 (6/34 children were lost in follow-up). No adverse effects during the treatment were reported.Conclusions: We were able to establish T. cruzi prevalence in the studied population and also to prove the usefulness of dried blood spot for T. cruzi detection using PCR in isolated areas. This method allowed us to verify early treatment failure. Possible causes of this failure are discussed below. Background: Parasitic infections by Trypanosoma cruzi (T. cruzi) are frequent in children from endemic areas. Specific therapies have been successfully used in pediatric populations to treat this disease. T. cruzi diagnosis should be optimized and become available for any clinical environment.Objective: To study T. cruzi prevalence in children from an area of active transmission and carry out a post-treatment follow-up. To verify the feasibility of detecting DNA ofT. cruzi from dried blood spot.Methods: We analyzed presence of T. cruzi in 78 Aboriginal children (Toba community) that attended to a rural school of Chaco province, Argentina. Serum and whole blood (dried blood spot) were assessed by means of serological techniques and PCR. Positive children received Benznidazole. Diagnosis and post treatment follow-up of T. cruzi infection were performed.Results: The serology assay showed infection in 34 of 78 (43.5%) children studied; PCR was positive in 5/34, displaying parasitemia. Serology remained positive in 28/28 children 120 days post-treatment, while PCR was positive in 18/28 (6/34 children were lost in follow-up). No adverse effects during the treatment were reported.Conclusions: We were able to establish T. cruzi prevalence in the studied population and also to prove the usefulness of dried blood spot for T. cruzi detection using PCR in isolated areas. This method allowed us to verify early treatment failure. Possible causes of this failure are discussed below. Universidad Nacional Córdoba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2016-10-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unc.edu.ar/index.php/med/article/view/14476 10.31053/1853.0605.v73.n3.14476 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 73 No. 3 (2016); 175-180 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 73 Núm. 3 (2016); 175-180 Revista da Faculdade de Ciências Médicas de Córdoba; v. 73 n. 3 (2016); 175-180 1853-0605 0014-6722 10.31053/1853.0605.v73.n3 eng https://revistas.unc.edu.ar/index.php/med/article/view/14476/15229 Derechos de autor 2016 Universidad Nacional de Córdoba https://creativecommons.org/licenses/by-nc/4.0

Ejemplares similares