Micropropagation of Anredera cordifolia (Basellaceae)
Background and aims: The potato vine (Anredera cordifolia) is a climbing plant of the Basellaceae family, with extensive potential for both food and medicinal purposes. The aim of this work was to develop an efficient micropropagation protocol for this species. M&M: Different factors that af...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Sociedad Argentina de Botánica
2024
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/44826 |
| Aporte de: |
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I10-R325-article-44826 |
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ojs |
| institution |
Universidad Nacional de Córdoba |
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I-10 |
| repository_str |
R-325 |
| container_title_str |
Boletín de la Sociedad Argentina de Botánica |
| language |
Español |
| format |
Artículo revista |
| topic |
Biotecnología. Anredera cordifolia Aclimatización Cultivo de tejidos Propagación in vitro |
| spellingShingle |
Biotecnología. Anredera cordifolia Aclimatización Cultivo de tejidos Propagación in vitro Schaller, Silvia C. Dolce, Natalia R. Mroginski, Luis A. Medina, Ricardo D. Micropropagation of Anredera cordifolia (Basellaceae) |
| topic_facet |
Biotecnología. Anredera cordifolia Aclimatización Cultivo de tejidos Propagación in vitro |
| author |
Schaller, Silvia C. Dolce, Natalia R. Mroginski, Luis A. Medina, Ricardo D. |
| author_facet |
Schaller, Silvia C. Dolce, Natalia R. Mroginski, Luis A. Medina, Ricardo D. |
| author_sort |
Schaller, Silvia C. |
| title |
Micropropagation of Anredera cordifolia (Basellaceae) |
| title_short |
Micropropagation of Anredera cordifolia (Basellaceae) |
| title_full |
Micropropagation of Anredera cordifolia (Basellaceae) |
| title_fullStr |
Micropropagation of Anredera cordifolia (Basellaceae) |
| title_full_unstemmed |
Micropropagation of Anredera cordifolia (Basellaceae) |
| title_sort |
micropropagation of anredera cordifolia (basellaceae) |
| description |
Background and aims: The potato vine (Anredera cordifolia) is a climbing plant of the Basellaceae family, with extensive potential for both food and medicinal purposes. The aim of this work was to develop an efficient micropropagation protocol for this species.
M&M: Different factors that affect the different stages of the process were evaluated, including adjustments in the disinfection of plant material for aseptic culture establishment, in vitro multiplication, and greenhouse acclimatization of the regenerated plants.
Results: For aseptic establishment, the most effective treatment in avoiding contamination, allowing explant survival, and promoting plant regeneration was the use of 1.7% active Cl. In the in vitro multiplication stage, the influence of the Murashige and Skoog (MS) basal medium and its dilutions were tested. It was demonstrated that the original concentration of MS was the most suitable for plant regeneration and promoted the highest rates of in vitro multiplication. In vitro plants derived from different dilutions of the MS basal medium were transferred to ex vitro growth conditions. Plants regenerated in MS reduced to a quarter exhibited the highest survival rate compared to the other treatments.
Conclusion: This study constitutes the first attempt to the micropropagation of A. cordifolia. This contribution is crucial for the establishment of an efficient large-scale propagation system as well as for germplasm preservation of this species. |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2024 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/44826 |
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AT schallersilviac micropropagationofanrederacordifoliabasellaceae AT dolcenataliar micropropagationofanrederacordifoliabasellaceae AT mroginskiluisa micropropagationofanrederacordifoliabasellaceae AT medinaricardod micropropagationofanrederacordifoliabasellaceae AT schallersilviac lamicropropagaciondeanrederacordifoliabasellaceae AT dolcenataliar lamicropropagaciondeanrederacordifoliabasellaceae AT mroginskiluisa lamicropropagaciondeanrederacordifoliabasellaceae AT medinaricardod lamicropropagaciondeanrederacordifoliabasellaceae |
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2025-02-05T22:20:45Z |
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2025-02-05T22:20:45Z |
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1827724389355880448 |
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I10-R325-article-448262024-12-30T13:31:50Z Micropropagation of Anredera cordifolia (Basellaceae) La Micropropagación de Anredera cordifolia (Basellaceae) Schaller, Silvia C. Dolce, Natalia R. Mroginski, Luis A. Medina, Ricardo D. Biotecnología. Anredera cordifolia Aclimatización Cultivo de tejidos Propagación in vitro Background and aims: The potato vine (Anredera cordifolia) is a climbing plant of the Basellaceae family, with extensive potential for both food and medicinal purposes. The aim of this work was to develop an efficient micropropagation protocol for this species. M&M: Different factors that affect the different stages of the process were evaluated, including adjustments in the disinfection of plant material for aseptic culture establishment, in vitro multiplication, and greenhouse acclimatization of the regenerated plants. Results: For aseptic establishment, the most effective treatment in avoiding contamination, allowing explant survival, and promoting plant regeneration was the use of 1.7% active Cl. In the in vitro multiplication stage, the influence of the Murashige and Skoog (MS) basal medium and its dilutions were tested. It was demonstrated that the original concentration of MS was the most suitable for plant regeneration and promoted the highest rates of in vitro multiplication. In vitro plants derived from different dilutions of the MS basal medium were transferred to ex vitro growth conditions. Plants regenerated in MS reduced to a quarter exhibited the highest survival rate compared to the other treatments. Conclusion: This study constitutes the first attempt to the micropropagation of A. cordifolia. This contribution is crucial for the establishment of an efficient large-scale propagation system as well as for germplasm preservation of this species. Introducción y Objetivos: La enredadera papa (Anredera cordifolia) es una planta escandente de la familia Basellaceae con amplio potencial alimenticio y medicinal. El objetivo del presente trabajo fue establecer un protocolo eficiente de micropropagación para esta especie. M&M: Se evaluaron diferentes factores que afectan las distintas etapas del proceso, el ajuste en la desinfección del material vegetal para el establecimiento aséptico del cultivo, la multiplicación in vitro y la aclimatización en invernadero de las plantas regeneradas. Resultados: El tratamiento de desinfección más efectivo para el establecimiento in vitro del material vegetal, evitando contaminaciones y permitiendo la supervivencia del explante y la regeneración de plantas, fue la utilización de 1,7% Cl activo. En la etapa de multiplicación in vitro se probó la influencia del medio basal de Murashige y Skoog (MS) y sus diluciones. Se demostró que el MS en su concentración original, resultó ser el más adecuado para la regeneración de plantas, y fue el que promovió las tasas más altas de multiplicación. Las plantas regeneradas in vitro derivadas de diferentes diluciones del medio basal MS fueron transferidas a las condiciones de crecimiento ex vitro. Las plantas provenientes de MS diluido a un cuarto, demostraron la supervivencia más alta en relación al resto de los tratamientos. Conclusión: Este trabajo constituye el primer aporte a la micropropagación de A. cordifolia. Esta contribución resulta crucial para el establecimiento de un sistema eficiente de propagación a gran escala, así como la conservación de germoplasma de esta especie. Sociedad Argentina de Botánica 2024-12-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/44826 10.31055/1851.2372.v59.n4.44826 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 59 No. 4 (2024): December Boletín de la Sociedad Argentina de Botánica; Vol. 59 Núm. 4 (2024): Diciembre Boletín de la Sociedad Argentina de Botánica; v. 59 n. 4 (2024): Diciembre 1851-2372 0373-580X 10.31055/1851.2372.v59.n4 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/44826/47591 https://revistas.unc.edu.ar/index.php/BSAB/article/view/44826/47592 Derechos de autor 2024 Silvia C Schaller, Natalia R. Dolce, Luis A. Mroginski, Ricardo D. Medina https://creativecommons.org/licenses/by-nc-nd/4.0 |