Development of microsatellite markers for the American species Vachellia aroma (Fabac
Background and aims: There are currently no microsatellite markers available for any American species of Vachellia, and particularly, for V. aroma. Then the aims of this study were to develop SSR markers specific for V. aroma, for the first time, and test its amplification in a close related species...
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| Formato: | Artículo revista |
| Lenguaje: | Inglés |
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Sociedad Argentina de Botánica
2024
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/40763 |
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I10-R325-article-407632024-03-28T23:23:56Z Development of microsatellite markers for the American species Vachellia aroma (Fabac Desarrollo de marcadores microsatélites para la especie americana Vachellia aroma (Fabaceae, Caesalpinodeae) Pometti, Carolina Luciana Bessega, Cecilia F. Ewens, Mauricio Vilardi, Juan C. Saidman, Beatriz O. 454 pyrosequencing polymorphic SSR Vachellia aroma, Vachellia caven 454 pirosecuenciación SSR polimórficos Vachellia aroma Background and aims: There are currently no microsatellite markers available for any American species of Vachellia, and particularly, for V. aroma. Then the aims of this study were to develop SSR markers specific for V. aroma, for the first time, and test its amplification in a close related species. M&M: For the development of the SSR in V. aroma, total genomic DNA was extracted and it was sequenced in a one-fourth run on a Roche 454 GS FLX+ platform. The study area included two Argentinean populations: San José and Robles, Santiago del Estero province. Results: We detected 422 sequences containing SSR loci. A set of 39 primer pairs presented amplified products in V. aroma and V. caven, but 12 revealed clear, replicable and polymorphic loci in V. aroma. Conclusions: The results of this work indicate that a new set of SSR markers was developed for V. aroma and their transferability to V. caven was assessed. The analysis of variability, showed that these 12 polymorphic markers are highly informative, and a powerful tool to investigate population genetics parameters in V. aroma and related species. Introducción y objetivos: Actualmente, no existen marcadores microsatélites disponibles para ninguna especie americana del género Vachellia, y particularmente, para V. aroma. Por lo tanto, los objetivos de este estudio fueron desarrollar marcadores SSR específicos para Vachellia aroma por primera vez, y probar su amplificación en una especie emparentada. M&M: Para el desarrollo de los microsatélites en V. aroma, se extrajo ADN genómico total de un individuo y este fue secuenciado en un cuarto de placa en una plataforma Roche 454 GS FLX+. El área de estudio comprendió dos poblaciones argentinas: San José y Robles, provincia de Santiago del Estero. Resultados: Detectamos 422 secuencias que contenían loci microsatélites. Un set de 39 pares de cebadores presentó amplificación de productos en Vachellia aroma y Vachellia caven, pero doce de ellos mostraron un patrón claro, reproducible y polimórfico en V. aroma. Conclusiones: Los resultados de este trabajo indican que se ha desarrollado un nuevo set de marcadores SSR específicos para V. aroma y su transferencia a V. caven fue exitosa. El análisis de variabilidad mostró que estos 12 marcadores polimórficos son altamente informativos, constituyendo una poderosa herramienta para investigar parámetros de la genética de poblaciones en V. aroma y especies relacionadas. Sociedad Argentina de Botánica 2024-03-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/40763 10.31055/1851.2372.v59.n1.40763 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 59 No. 1 (2024): March Boletín de la Sociedad Argentina de Botánica; Vol. 59 Núm. 1 (2024): Marzo Boletín de la Sociedad Argentina de Botánica; v. 59 n. 1 (2024): Marzo 1851-2372 0373-580X 10.31055/1851.2372.v59.n1 eng https://revistas.unc.edu.ar/index.php/BSAB/article/view/40763/44787 https://revistas.unc.edu.ar/index.php/BSAB/article/view/40763/44788 Derechos de autor 2024 Carolina Luciana Pometti, Cecilia Bessega, Mauricio Ewens, Juan C. Vilardi, Beatriz O. Saidman https://creativecommons.org/licenses/by-nc-nd/4.0 |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-325 |
| container_title_str |
Boletín de la Sociedad Argentina de Botánica |
| language |
Inglés |
| format |
Artículo revista |
| topic |
454 pyrosequencing polymorphic SSR Vachellia aroma, Vachellia caven 454 pirosecuenciación SSR polimórficos Vachellia aroma |
| spellingShingle |
454 pyrosequencing polymorphic SSR Vachellia aroma, Vachellia caven 454 pirosecuenciación SSR polimórficos Vachellia aroma Pometti, Carolina Luciana Bessega, Cecilia F. Ewens, Mauricio Vilardi, Juan C. Saidman, Beatriz O. Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| topic_facet |
454 pyrosequencing polymorphic SSR Vachellia aroma, Vachellia caven 454 pirosecuenciación SSR polimórficos Vachellia aroma |
| author |
Pometti, Carolina Luciana Bessega, Cecilia F. Ewens, Mauricio Vilardi, Juan C. Saidman, Beatriz O. |
| author_facet |
Pometti, Carolina Luciana Bessega, Cecilia F. Ewens, Mauricio Vilardi, Juan C. Saidman, Beatriz O. |
| author_sort |
Pometti, Carolina Luciana |
| title |
Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| title_short |
Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| title_full |
Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| title_fullStr |
Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| title_full_unstemmed |
Development of microsatellite markers for the American species Vachellia aroma (Fabac |
| title_sort |
development of microsatellite markers for the american species vachellia aroma (fabac |
| description |
Background and aims: There are currently no microsatellite markers available for any American species of Vachellia, and particularly, for V. aroma. Then the aims of this study were to develop SSR markers specific for V. aroma, for the first time, and test its amplification in a close related species.
M&M: For the development of the SSR in V. aroma, total genomic DNA was extracted and it was sequenced in a one-fourth run on a Roche 454 GS FLX+ platform. The study area included two Argentinean populations: San José and Robles, Santiago del Estero province.
Results: We detected 422 sequences containing SSR loci. A set of 39 primer pairs presented amplified products in V. aroma and V. caven, but 12 revealed clear, replicable and polymorphic loci in V. aroma.
Conclusions: The results of this work indicate that a new set of SSR markers was developed for V. aroma and their transferability to V. caven was assessed. The analysis of variability, showed that these 12 polymorphic markers are highly informative, and a powerful tool to investigate population genetics parameters in V. aroma and related species. |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2024 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/40763 |
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