Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)

Background and aims: Zephyranthes mesochloa, is a bulbous species with white flowers that inhabits the subtropical region of South America and presents different chromosome numbers. The aim of this work is to carry out the chromosome analysis of Z. mesochloa from northern Argentina, to contribute to...

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Autores principales: Gianini Aquino, Analia C., Rodriguez Mata, Orlando A., Honfi, Ana I., Daviña, Julio Rubén
Formato: Artículo revista
Lenguaje:Español
Publicado: Sociedad Argentina de Botánica 2023
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Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/40181
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institution Universidad Nacional de Córdoba
institution_str I-10
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container_title_str Boletín de la Sociedad Argentina de Botánica
language Español
format Artículo revista
topic autopoloploidía
cromosomas
cromosoma B
cariotipo
meiosis
plantas bulbosas ornamentales
autopolyploidy
B chromosome
chromosomes
karyotype
meiosis
ornamental bulb plant
spellingShingle autopoloploidía
cromosomas
cromosoma B
cariotipo
meiosis
plantas bulbosas ornamentales
autopolyploidy
B chromosome
chromosomes
karyotype
meiosis
ornamental bulb plant
Gianini Aquino, Analia C.
Rodriguez Mata, Orlando A.
Honfi, Ana I.
Daviña, Julio Rubén
Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
topic_facet autopoloploidía
cromosomas
cromosoma B
cariotipo
meiosis
plantas bulbosas ornamentales
autopolyploidy
B chromosome
chromosomes
karyotype
meiosis
ornamental bulb plant
author Gianini Aquino, Analia C.
Rodriguez Mata, Orlando A.
Honfi, Ana I.
Daviña, Julio Rubén
author_facet Gianini Aquino, Analia C.
Rodriguez Mata, Orlando A.
Honfi, Ana I.
Daviña, Julio Rubén
author_sort Gianini Aquino, Analia C.
title Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
title_short Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
title_full Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
title_fullStr Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
title_full_unstemmed Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae)
title_sort cytogenetic studies in the polyploid complex zephyranthes mesochloa (amaryllidaceae)
description Background and aims: Zephyranthes mesochloa, is a bulbous species with white flowers that inhabits the subtropical region of South America and presents different chromosome numbers. The aim of this work is to carry out the chromosome analysis of Z. mesochloa from northern Argentina, to contribute to the cytogenetic knowledge of the genus. M&M: Ploidy levels and somatic chromosome numbers were determined in individuals from 8 populations in northern Argentina using conventional staining. In addition, meiotic preparations with acetic carmine were performed on individuals from one population. Results: Seventy-five percent of the populations were diploid, with 2n = 2x = 12 and presented a karyotypic formula of 4m + 4sm + 4st. In two of the diploid populations, individuals with 2n = 13 were observed, with the presence of an additional metacentric chromosome, possibly a B chromosome. Microsporogenesis analysis, in diakinesis and metaphase I, of aneuploid cytotype (2n = 13), showed six bivalents and one univalent in all pollen mother cells (PMCs) analyzed. The remaining populations (25%) were tetraploids with 2n = 4x = 24 and karyotypic formula 8m + 8sm + 8st. Geographically, the distribution of cytotypes in Z. mesochloa is discontinuous. Conclusions: The duplicate karyotype formula in tetraploids regarding its con-specific diploids may indicate the origin of an autopolyploid cytotype. The available data allows us to consider the group of cytotypes as part of a polyploid complex with a basic number of x = 6.
publisher Sociedad Argentina de Botánica
publishDate 2023
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/40181
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first_indexed 2024-09-03T22:50:27Z
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spelling I10-R325-article-401812023-11-15T16:43:29Z Cytogenetic studies in the polyploid complex Zephyranthes mesochloa (Amaryllidaceae) Estudios citogenéticos en el complejo poliploide Zephyranthes mesochloa (Amaryllidaceae) Gianini Aquino, Analia C. Rodriguez Mata, Orlando A. Honfi, Ana I. Daviña, Julio Rubén autopoloploidía cromosomas cromosoma B cariotipo meiosis plantas bulbosas ornamentales autopolyploidy B chromosome chromosomes karyotype meiosis ornamental bulb plant Background and aims: Zephyranthes mesochloa, is a bulbous species with white flowers that inhabits the subtropical region of South America and presents different chromosome numbers. The aim of this work is to carry out the chromosome analysis of Z. mesochloa from northern Argentina, to contribute to the cytogenetic knowledge of the genus. M&M: Ploidy levels and somatic chromosome numbers were determined in individuals from 8 populations in northern Argentina using conventional staining. In addition, meiotic preparations with acetic carmine were performed on individuals from one population. Results: Seventy-five percent of the populations were diploid, with 2n = 2x = 12 and presented a karyotypic formula of 4m + 4sm + 4st. In two of the diploid populations, individuals with 2n = 13 were observed, with the presence of an additional metacentric chromosome, possibly a B chromosome. Microsporogenesis analysis, in diakinesis and metaphase I, of aneuploid cytotype (2n = 13), showed six bivalents and one univalent in all pollen mother cells (PMCs) analyzed. The remaining populations (25%) were tetraploids with 2n = 4x = 24 and karyotypic formula 8m + 8sm + 8st. Geographically, the distribution of cytotypes in Z. mesochloa is discontinuous. Conclusions: The duplicate karyotype formula in tetraploids regarding its con-specific diploids may indicate the origin of an autopolyploid cytotype. The available data allows us to consider the group of cytotypes as part of a polyploid complex with a basic number of x = 6. Introducción y objetivos: Zephyranthes mesochloa es una especie bulbosa de flores blancas que habita en la región subtropical del sur de Sudamérica y registra distintos números cromosómicos. El objetivo del presente trabajo es analizar cromosómicamente individuos de la especie Z. mesochloa del norte de Argentina, con el objeto de contribuir al conocimiento citogenético del género.  M&M: El nivel de ploidía y el número cromosómico somático se determinó en individuos procedentes de 8 poblaciones del norte de Argentina a partir de tinción convencional. Además, se realizaron preparaciones meióticas con carmín acético en individuos de una de las poblaciones.  Resultados: El 75% de las poblaciones resultaron diploides, con 2n = 2x = 12 y fórmula cariotípica 4m + 4sm + 4st. En dos de las poblaciones diploides se observaron individuos con 2n = 13, con presencia de un cromosoma metacéntrico adicional, que posiblemente se trate de un cromosoma B. El análisis de la microsporogénesis, en diacinesis y metafase I, del citotipo aneuploide (2n = 13), mostró 6 bivalentes y 1 univalente en todas las células madre del polen (CMP) analizadas. Las poblaciones restantes (25%), resultaron tetraploides con 2n = 4x = 24 y fórmula cariotípica 8m + 8sm + 8st. Geográficamente, la distribución de los citotipos de Z. mesochloa es discontinua. Conclusiones: La fórmula cariotípica duplicada en los tetraploides con respecto a los diploides conespecíficos indicarían un origen autopoliploide de este citotipo. El conjunto de datos disponibles permite considerar al conjunto de citotipos como un complejo poliploide con número básico x = 6. Sociedad Argentina de Botánica 2023-08-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/40181 10.31055/1851.2372.v58.n3.40181 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 58 No. 3 (2023): September. Special Issue: Amaryllidaceae Boletín de la Sociedad Argentina de Botánica; Vol. 58 Núm. 3 (2023): Septiembre. Número especial: Amaryllidaceae Boletín de la Sociedad Argentina de Botánica; v. 58 n. 3 (2023): Setembro. Edição especial: Amaryllidaceae 1851-2372 0373-580X 10.31055/1851.2372.v58.n3 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/40181/42409 https://revistas.unc.edu.ar/index.php/BSAB/article/view/40181/42410 Derechos de autor 2023 Analia C. Gianini Aquino, Orlando A. Rodriguez Mata, Ana I. Honfi, Julio Rubén Daviña https://creativecommons.org/licenses/by-nc-nd/4.0