Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen

Background and aims: Colletotrichum graminicola (Glomerellaceae, Glomerellales), the causal agent of maize (Zea mays) anthracnose, as many other fungal pathogens, relies on its battery of cell wall degrading enzymes (CWDEs) to make its way through the cell walls of the host, and thus the production...

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Autores principales: Nuñez, María del Pilar, Levin, Laura Noemí, Cinto, Isabel Esther
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Sociedad Argentina de Botánica 2023
Materias:
Antracnosis
Colletotrichum graminicola
Enzimas degradadoras de pared celular
Maíz
Anthracnose
Cell wall degrading enzymes
Maize
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/39049
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
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institution Universidad Nacional de Córdoba
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container_title_str Boletín de la Sociedad Argentina de Botánica
language Inglés
format Artículo revista
topic Antracnosis
Colletotrichum graminicola
Enzimas degradadoras de pared celular
Maíz
Anthracnose
Cell wall degrading enzymes
Colletotrichum graminicola
Maize
spellingShingle Antracnosis
Colletotrichum graminicola
Enzimas degradadoras de pared celular
Maíz
Anthracnose
Cell wall degrading enzymes
Colletotrichum graminicola
Maize
Nuñez, María del Pilar
Levin, Laura Noemí
Cinto, Isabel Esther
Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
topic_facet Antracnosis
Colletotrichum graminicola
Enzimas degradadoras de pared celular
Maíz
Anthracnose
Cell wall degrading enzymes
Colletotrichum graminicola
Maize
author Nuñez, María del Pilar
Levin, Laura Noemí
Cinto, Isabel Esther
author_facet Nuñez, María del Pilar
Levin, Laura Noemí
Cinto, Isabel Esther
author_sort Nuñez, María del Pilar
title Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
title_short Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
title_full Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
title_fullStr Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
title_full_unstemmed Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen
title_sort intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of colletotrichum graminicola, a maize pathogen
description Background and aims: Colletotrichum graminicola (Glomerellaceae, Glomerellales), the causal agent of maize (Zea mays) anthracnose, as many other fungal pathogens, relies on its battery of cell wall degrading enzymes (CWDEs) to make its way through the cell walls of the host, and thus the production of these enzymes is considered a virulence factor. The aim of this work was to investigate if there is intraspecific variability in growth and in vitro production of several extracellular CWDEs among Argentinean fungal isolates of C. graminicola. M&M: Eight isolates of C. graminicola were tested in vitro to evaluate growth capacity and polygalacturonase, polymethylgalacturonase, β-glucosidase and laccase production, using two different liquid culture media. Results: Polygalacturonase, polymethylgalacturonase and β-glucosidase production greatly varied among isolates and culture media. Laccase activity was detected only in three isolates. Utmost enzymatic titres attained were respectively 250, 280, 45 and 63 U/l. The observed intraspecific variability in CWDEs in vitro production is consistent with the high variability found at genetic level when assessing ISSR markers. Conclusions: The isolates of C. graminicola evaluated showed notable differences in CWDEs production, not associated with a differential growth. This indicates a large intraspecific variability, which might be considered when choosing a method to deal with this pathogen.
publisher Sociedad Argentina de Botánica
publishDate 2023
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/39049
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first_indexed 2024-09-03T22:50:24Z
last_indexed 2024-09-03T22:50:24Z
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spelling I10-R325-article-390492023-07-03T18:09:55Z Intraspecific variability in growth and in vitro production of plant cell wall-degrading enzymes among argentinean isolates of Colletotrichum graminicola, a maize pathogen Variabilidad intraespecífica en el crecimiento y la producción in vitro de enzimas degradadoras de pared celular vegetal entre aislamientos argentinos de Colletotrichum graminicola, un patógeno de maíz Nuñez, María del Pilar Levin, Laura Noemí Cinto, Isabel Esther Antracnosis Colletotrichum graminicola Enzimas degradadoras de pared celular Maíz Anthracnose Cell wall degrading enzymes Colletotrichum graminicola Maize Background and aims: Colletotrichum graminicola (Glomerellaceae, Glomerellales), the causal agent of maize (Zea mays) anthracnose, as many other fungal pathogens, relies on its battery of cell wall degrading enzymes (CWDEs) to make its way through the cell walls of the host, and thus the production of these enzymes is considered a virulence factor. The aim of this work was to investigate if there is intraspecific variability in growth and in vitro production of several extracellular CWDEs among Argentinean fungal isolates of C. graminicola. M&M: Eight isolates of C. graminicola were tested in vitro to evaluate growth capacity and polygalacturonase, polymethylgalacturonase, β-glucosidase and laccase production, using two different liquid culture media. Results: Polygalacturonase, polymethylgalacturonase and β-glucosidase production greatly varied among isolates and culture media. Laccase activity was detected only in three isolates. Utmost enzymatic titres attained were respectively 250, 280, 45 and 63 U/l. The observed intraspecific variability in CWDEs in vitro production is consistent with the high variability found at genetic level when assessing ISSR markers. Conclusions: The isolates of C. graminicola evaluated showed notable differences in CWDEs production, not associated with a differential growth. This indicates a large intraspecific variability, which might be considered when choosing a method to deal with this pathogen. Introducción y objetivos: Colletotrichum graminicola (Glomerellaceae, Glomerellales) el agente causal de la antracnosis del maíz es dependiente de la actividad de enzimas degradadoras de la pared celular vegetal, para penetrar en su hospedante. La producción de estas enzimas se considera un factor de virulencia. El objetivo del presente trabajo fue investigar si existe variabilidad entre aislamientos en la capacidad de crecimiento y producción in vitro de diversas enzimas involucradas en la degradación de pared celular vegetal. M&M: Se evaluó la habilidad de ocho aislamientos de C. graminícola para crecer y sintetizar enzimas con actividad poligalacturonasa, polimetilgalacturonasa, β-glucosidasa y lacasa en cultivos líquidos utilizando dos medios de diferente composición. Resultados: La producción de poligalacturonasa, polimetilgalacturonasa y β-glucosidasa difirió marcadamente entre aislamientos y medios de cultivo. Se detectó actividad lacasa sólo en tres de los aislamientos. Los máximos títulos enzimáticos obtenidos fueron respectivamente de 250, 280, 45 y 63 U/l. La variabilidad intraespecífica registrada en la producción enzimática es consistente con la alta variabilidad intraespecífica observada a nivel genético cuando se evaluaron marcadores moleculares ISSR. Conclusiones: Los aislamientos de C. graminicola investigados mostraron notables diferencias en cuanto a la producción de enzimas degradadoras de pared celular vegetal, no asociadas a su capacidad de crecimiento. Esto indica una importante variabilidad intraespecífica que debería tenerse en cuenta al seleccionar un método para combatir a este patógeno. Sociedad Argentina de Botánica 2023-05-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/39049 10.31055/1851.2372.v58.n2.39049 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 58 No. 2 (2023): June Boletín de la Sociedad Argentina de Botánica; Vol. 58 Núm. 2 (2023): Junio Boletín de la Sociedad Argentina de Botánica; v. 58 n. 2 (2023): Junio 1851-2372 0373-580X 10.31055/1851.2372.v58.n2 eng https://revistas.unc.edu.ar/index.php/BSAB/article/view/39049/41262 https://revistas.unc.edu.ar/index.php/BSAB/article/view/39049/41263 Derechos de autor 2023 María del Pilar Nuñez, Laura Noemí Levin, Isabel Esther Cinto https://creativecommons.org/licenses/by-nc-nd/4.0

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