Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)

Background and aims: Zephyranthes brachyandra belongs to a tribe of ornamental Amaryllidaceae native of South America, whose genera circumscription and phylogenetic relationships are still unclear. Cytologically, Z. brachyandra is a tetraploid whose chromosomes are of similar size and morphology, hi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Nascimento, Thiago Henrique, Gonçalves, Raquel S., Báez, Mariana, Seijo, Guillermo, Guerra, Marcelo
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Sociedad Argentina de Botánica 2022
Materias:
CMA/DAPI banding
cytotaxonomy
FISH
monotrisomy
rDNA
telomeric DNA
Bandeamiento CMA/DAPI
citotaxonomía
monotrisomía
ADNr, ADN telomérico
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
id I10-R325-article-34304
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-325
container_title_str Boletín de la Sociedad Argentina de Botánica
language Inglés
format Artículo revista
topic CMA/DAPI banding
cytotaxonomy
FISH
monotrisomy
rDNA
telomeric DNA
Bandeamiento CMA/DAPI
citotaxonomía
monotrisomía
ADNr, ADN telomérico
spellingShingle CMA/DAPI banding
cytotaxonomy
FISH
monotrisomy
rDNA
telomeric DNA
Bandeamiento CMA/DAPI
citotaxonomía
monotrisomía
ADNr, ADN telomérico
Nascimento, Thiago Henrique
Gonçalves, Raquel S.
Báez, Mariana
Seijo, Guillermo
Guerra, Marcelo
Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
topic_facet CMA/DAPI banding
cytotaxonomy
FISH
monotrisomy
rDNA
telomeric DNA
Bandeamiento CMA/DAPI
citotaxonomía
monotrisomía
ADNr, ADN telomérico
author Nascimento, Thiago Henrique
Gonçalves, Raquel S.
Báez, Mariana
Seijo, Guillermo
Guerra, Marcelo
author_facet Nascimento, Thiago Henrique
Gonçalves, Raquel S.
Báez, Mariana
Seijo, Guillermo
Guerra, Marcelo
author_sort Nascimento, Thiago Henrique
title Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
title_short Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
title_full Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
title_fullStr Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
title_full_unstemmed Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae)
title_sort molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in zephyranthes brachyandra (amaryllidaceae)
description Background and aims: Zephyranthes brachyandra belongs to a tribe of ornamental Amaryllidaceae native of South America, whose genera circumscription and phylogenetic relationships are still unclear. Cytologically, Z. brachyandra is a tetraploid whose chromosomes are of similar size and morphology, hindering the identification of its 2n = 24 chromosomes. The aim of this study was to investigate the stability of the many CMA+ and DAPI+ bands and the occurrence of B chromosomes by a cytomolecular approach.  M&M: For this investigation we conducted a cytomolecular analysis with CMA/DAPI staining and fluorescence in situ hybridization with 5S and 35S rDNA probes, and the TTTAGGG telomeric probe. Results: In the present work, a cytomolecular analysis of Z. brachyandra, revealed a large and variable number of CMA+ and DAPI+ heterochromatic bands and 5S and 35S rDNA sites, and a regular distribution of the TTTAGGG telomeric sequences. In addition, one individual was monotrisomic with 2n = 24, and another one had a B chromosome. Both numerical and structural chromosome alterations were clearly characterized by CMA/DAPI bands and rDNA sites. Conclusions: Comparing the present data with the cytological data for other species of Zephyranthes, it becomes clear that a cytomolecular approach is fundamental to the understanding of the chromosome variation and cytotaxonomy of the group.
publisher Sociedad Argentina de Botánica
publishDate 2022
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304
work_keys_str_mv AT nascimentothiagohenrique molecularcytogeneticsrevealsanuncommonstructuralandnumericalchromosomalheteromorphisminzephyranthesbrachyandraamaryllidaceae
AT goncalvesraquels molecularcytogeneticsrevealsanuncommonstructuralandnumericalchromosomalheteromorphisminzephyranthesbrachyandraamaryllidaceae
AT baezmariana molecularcytogeneticsrevealsanuncommonstructuralandnumericalchromosomalheteromorphisminzephyranthesbrachyandraamaryllidaceae
AT seijoguillermo molecularcytogeneticsrevealsanuncommonstructuralandnumericalchromosomalheteromorphisminzephyranthesbrachyandraamaryllidaceae
AT guerramarcelo molecularcytogeneticsrevealsanuncommonstructuralandnumericalchromosomalheteromorphisminzephyranthesbrachyandraamaryllidaceae
AT nascimentothiagohenrique lacitogeneticamolecularrevelaunheteromorfismocromosomiconumericoyestructuralpococomunenzephyranthesbrachyandraamaryllidaceae
AT goncalvesraquels lacitogeneticamolecularrevelaunheteromorfismocromosomiconumericoyestructuralpococomunenzephyranthesbrachyandraamaryllidaceae
AT baezmariana lacitogeneticamolecularrevelaunheteromorfismocromosomiconumericoyestructuralpococomunenzephyranthesbrachyandraamaryllidaceae
AT seijoguillermo lacitogeneticamolecularrevelaunheteromorfismocromosomiconumericoyestructuralpococomunenzephyranthesbrachyandraamaryllidaceae
AT guerramarcelo lacitogeneticamolecularrevelaunheteromorfismocromosomiconumericoyestructuralpococomunenzephyranthesbrachyandraamaryllidaceae
first_indexed 2024-09-03T22:50:09Z
last_indexed 2024-09-03T22:50:09Z
_version_ 1809217024593756160
spelling I10-R325-article-343042022-04-22T13:03:00Z Molecular cytogenetics reveals an uncommon structural and numerical chromosomal heteromorphism in Zephyranthes brachyandra (Amaryllidaceae) La citogenética molecular revela un heteromorfismo cromosómico numérico y estructural poco común en Zephyranthes brachyandra (Amaryllidaceae) Nascimento, Thiago Henrique Gonçalves, Raquel S. Báez, Mariana Seijo, Guillermo Guerra, Marcelo CMA/DAPI banding cytotaxonomy FISH monotrisomy rDNA telomeric DNA Bandeamiento CMA/DAPI citotaxonomía monotrisomía ADNr, ADN telomérico Background and aims: Zephyranthes brachyandra belongs to a tribe of ornamental Amaryllidaceae native of South America, whose genera circumscription and phylogenetic relationships are still unclear. Cytologically, Z. brachyandra is a tetraploid whose chromosomes are of similar size and morphology, hindering the identification of its 2n = 24 chromosomes. The aim of this study was to investigate the stability of the many CMA+ and DAPI+ bands and the occurrence of B chromosomes by a cytomolecular approach.  M&M: For this investigation we conducted a cytomolecular analysis with CMA/DAPI staining and fluorescence in situ hybridization with 5S and 35S rDNA probes, and the TTTAGGG telomeric probe. Results: In the present work, a cytomolecular analysis of Z. brachyandra, revealed a large and variable number of CMA+ and DAPI+ heterochromatic bands and 5S and 35S rDNA sites, and a regular distribution of the TTTAGGG telomeric sequences. In addition, one individual was monotrisomic with 2n = 24, and another one had a B chromosome. Both numerical and structural chromosome alterations were clearly characterized by CMA/DAPI bands and rDNA sites. Conclusions: Comparing the present data with the cytological data for other species of Zephyranthes, it becomes clear that a cytomolecular approach is fundamental to the understanding of the chromosome variation and cytotaxonomy of the group. Introducción y objetivos: Zephyranthes brachyandra pertenece a una tribu de Amaryllidaceae ornamentales nativa de América del Sur, cuya circunscripción de géneros y relaciones filogenéticas aún no están claras. Citológicamente, Z.brachyandra es un tetraploide cuyos cromosomas son de tamaño y morfología similar, lo que dificulta la identificación de sus 2n = 24 cromosomas. El objetivo de este estudio fue investigar la estabilidad de las numerosas bandas CMA+ y DAPI+ y la aparición de cromosomas B mediante un enfoque citomolecular. M&M: Para esta investigación realizamos un análisis citomolecular con tinción CMA/DAPI e hibridación fluorescente in situ con sondas de ADNr 5S y 35S, y la sondatelomérica TTTAGGG. Resultados: En el presente trabajo se realizaron varios análisis citomoleculares de Z.brachyandra, que revelaron un número alto y variable de bandas heterocromáticasCMA+ y DAPI+ y de sitios de ADNr 5S y 35S, además de una distribución típica de las secuencias teloméricas TTTAGGG. Además, un individuo era monotrisómico con 2n = 24 y otro tenía un cromosoma B. Las alteraciones cromosómicas tanto numéricas como estructurales se caracterizaron claramente por bandas CMA/DAPI y sitios de ADNr. Conclusión: Al comparar los datos actuales con la literatura citológica de otras especies del género Zephyranthes, queda claro que un enfoque citomolecular es fundamental para la comprensión de la variación cromosómica y la citotaxonomía del grupo.   Sociedad Argentina de Botánica 2022-03-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304 10.31055/1851.2372.v57.n1.34304 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 57 No. 1 (2022): March Boletín de la Sociedad Argentina de Botánica; Vol. 57 Núm. 1 (2022): Marzo Boletín de la Sociedad Argentina de Botánica; v. 57 n. 1 (2022): Marzo 1851-2372 0373-580X 10.31055/1851.2372.v57.n1 eng https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304/37439 https://revistas.unc.edu.ar/index.php/BSAB/article/view/34304/37440 Derechos de autor 2022 Thiago Henrique Nascimento, Raquel S. Gonçalves, Mariana Báez, Guillermo Seijo, Marcelo Guerra https://creativecommons.org/licenses/by-nc-nd/4.0

Ejemplares similares