Xylariales): Physiological characterization and structural features of its association with wheat.

: Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, whic...

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Autores principales: Rothen, Carolina, Miranda, Victoria, Fracchia, Sebastián, Godeas, Alicia, Rodríguez, Alejandra
Formato: Artículo revista
Lenguaje:Inglés
Publicado: Sociedad Argentina de Botánica 2018
Materias:
Endofitos septados oscuros
hongos endofitos
Microdochium bolleyi
trigo.
Dark septate endophytes
root endophytes
wheat crop
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/20574
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
id I10-R325-article-20574
record_format ojs
spelling I10-R325-article-205742019-12-14T13:46:36Z Xylariales): Physiological characterization and structural features of its association with wheat. Microdochium bolleyi (Ascomycota: Xylariales): Caracterización fisiológica y caracteres estructurales de su asociación con trigo Rothen, Carolina Miranda, Victoria Fracchia, Sebastián Godeas, Alicia Rodríguez, Alejandra Endofitos septados oscuros hongos endofitos Microdochium bolleyi trigo. Dark septate endophytes Microdochium bolleyi root endophytes wheat crop : Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogens in cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina, and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole and to solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesis was performed under controlled conditions in order to characterize root fungal colonization under both, optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, forming chlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogen sources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Only amylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain 22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a “true endophyte”; however further studies are necessary to elucidate its role in the association with wheat. Microdochium bolleyi (Ascomycota: Xylariales): Caracterización fisiológica y caracteres estructurales de su asociación con trigo. Las raíces de las plantas hospedan una gran diversidad de hongos, entre ellos, se encuentran los Endofitos Septados Oscuros (ESO). Microdochium bolleyi coloniza las raíces de trigo y otros cereales, aunque algunos autores lo han considerado un patógeno débil, otros han demostrado su acción biocontroladora contra patógenos agresivos del suelo. En el presente trabajo, se aisló una cepa de M. bolleyi (22-1) de raíces de trigo. Esta cepa fue metabólicamente caracterizada y se realizó un ensayo de resíntesis bajo condiciones controladas con el fin de caracterizar la colonización del hongo en la raíz bajo microscopía óptica y de transmisión. Su crecimiento fue escaso en las fuentes de carbono y nitrógeno evaluadas, sintetizó indoles en cultivo in vitro, pero no mostró habilidades para solubilizar el fósforo, por último, solo se detectó actividad amilasa. La cepa 22-1 coloniza la corteza radicular del trigo, formando clamidosporas melanizadas inter e intracelularmente y en el interior de los pelos radiculares. Microdochium bolleyi (cepa 22-1) coloniza la raíz de trigo formando las típicas estructuras de los ESO y comportándose como un “verdadero endófito”, sin embargo, se necesitan más estudios para terminar de dilucidar su papel en la asociación con el trigo. Sociedad Argentina de Botánica 2018-07-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original application/pdf https://revistas.unc.edu.ar/index.php/BSAB/article/view/20574 10.31055/1851.2372.v53.n2.20574 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 53 No. 2 (2018): June; 169-182 Boletín de la Sociedad Argentina de Botánica; Vol. 53 Núm. 2 (2018): Junio; 169-182 Boletín de la Sociedad Argentina de Botánica; v. 53 n. 2 (2018): Junio; 169-182 1851-2372 0373-580X 10.31055/1851.2372.v53.n2 eng https://revistas.unc.edu.ar/index.php/BSAB/article/view/20574/20246 Derechos de autor 2018 Boletín de la Sociedad Argentina de Botánica
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-325
container_title_str Boletín de la Sociedad Argentina de Botánica
language Inglés
format Artículo revista
topic Endofitos septados oscuros
hongos endofitos
Microdochium bolleyi
trigo.
Dark septate endophytes
Microdochium bolleyi
root endophytes
wheat crop
spellingShingle Endofitos septados oscuros
hongos endofitos
Microdochium bolleyi
trigo.
Dark septate endophytes
Microdochium bolleyi
root endophytes
wheat crop
Rothen, Carolina
Miranda, Victoria
Fracchia, Sebastián
Godeas, Alicia
Rodríguez, Alejandra
Xylariales): Physiological characterization and structural features of its association with wheat.
topic_facet Endofitos septados oscuros
hongos endofitos
Microdochium bolleyi
trigo.
Dark septate endophytes
Microdochium bolleyi
root endophytes
wheat crop
author Rothen, Carolina
Miranda, Victoria
Fracchia, Sebastián
Godeas, Alicia
Rodríguez, Alejandra
author_facet Rothen, Carolina
Miranda, Victoria
Fracchia, Sebastián
Godeas, Alicia
Rodríguez, Alejandra
author_sort Rothen, Carolina
title Xylariales): Physiological characterization and structural features of its association with wheat.
title_short Xylariales): Physiological characterization and structural features of its association with wheat.
title_full Xylariales): Physiological characterization and structural features of its association with wheat.
title_fullStr Xylariales): Physiological characterization and structural features of its association with wheat.
title_full_unstemmed Xylariales): Physiological characterization and structural features of its association with wheat.
title_sort xylariales): physiological characterization and structural features of its association with wheat.
description : Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogens in cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina, and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole and to solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesis was performed under controlled conditions in order to characterize root fungal colonization under both, optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, forming chlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogen sources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Only amylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain 22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a “true endophyte”; however further studies are necessary to elucidate its role in the association with wheat.
publisher Sociedad Argentina de Botánica
publishDate 2018
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/20574
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AT fracchiasebastian xylarialesphysiologicalcharacterizationandstructuralfeaturesofitsassociationwithwheat
AT godeasalicia xylarialesphysiologicalcharacterizationandstructuralfeaturesofitsassociationwithwheat
AT rodriguezalejandra xylarialesphysiologicalcharacterizationandstructuralfeaturesofitsassociationwithwheat
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first_indexed 2024-09-03T22:49:05Z
last_indexed 2024-09-03T22:49:05Z
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