Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).

Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota). In roots of adult tomato plants a wet and brown lesion, extending 2-4 cm above the soil was observed. Diseased plants withered and died. The objective of this paper was to isolate and to identify the causal organi...

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Autores principales: Grijalba, Pablo E., Zapata, Raúl L., Palmucci, Hemilse E, Baron, Claudio
Formato: Artículo revista
Lenguaje:Español
Publicado: Sociedad Argentina de Botánica 2015
Materias:
Oomycete
Pythium
Tomate
Tomato
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/10843
Aporte de:
Boletín de la Sociedad Argentina de Botánica de Universidad Nacional de Córdoba
id I10-R325-article-10843
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spelling I10-R325-article-108432024-09-03T22:47:32Z Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota). Podredumbre basal de plantas adultas de tomate causada por Pythium aphanidermatum (Oomycota) Grijalba, Pablo E. Zapata, Raúl L. Palmucci, Hemilse E Baron, Claudio Oomycete Pythium Tomate Oomycete Pythium Tomato Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota). In roots of adult tomato plants a wet and brown lesion, extending 2-4 cm above the soil was observed. Diseased plants withered and died. The objective of this paper was to isolate and to identify the causal organism of these symptoms. General and selective culture media were used. After 24 hours colonies with white cottony mycelium were developed. The morphology of the colony and the production, morphology and size of reproductive structures were evaluated. The rDNA extraction was made from mycelium of pure cultures 7-10 days old grown in agarized media. The ITS region was amplified using ITS4 and ITS5 primers, sequenced and compared in gene bank - NCBI BLAST server to verify their sequence similarity to the type or holotype available. Coenocytic mycelium, spherical-globular sporangium and aplerotic oospores, characteristic of the genus Pythium were observed in microscopic slides. Pathogenicity tests were conducted with positive results; a microorganism identical to the original was re-isolated. The characterization achieved by traditional techniques, molecular studies and the results of the pathogenicity tests support the conclusion that Pythium aphanidermatum is the causal agent of basal rot on mature plants of tomato En raíces de plantas adultas de tomate se observó una lesión castaña de aspecto húmedo, que se extendía 2-4 cm por encima del suelo. Las plantas atacadas se marchitaron y murieron. El objetivo del trabajo fue aislar e identificar al organismo causal de esta sintomatología. Se usaron medios de cultivos generales y selectivos. A las 24 horas desarrollaron colonias con micelio blanco algodonoso. Se evaluó la morfología de la colonia y la producción, morfología y dimensiones de estructuras reproductivas. El rDNA del micelio de un aislamiento fue extraído de cultivos puros con 7-10 días de crecimiento en medios agarizados. La región ITS se amplificó usando primers ITS4 e ITS5, se secuenció y comparó en banco de genes del servidor BLAST- NCBI para verificar su similitud con las secuencias tipo u holotipo disponibles. Al microscopio se observó micelio cenocítico, esporangios esfericos-globulados y oosporas apleroticas característicos del género Pythium. Se efectuaron pruebas de patogenicidad con resultados positivos reaislándose un microorganismo de características idénticas. La caracterización lograda por las técnicas tradicionales, los estudios moleculares y el resultado de las pruebas de patogenicidad permiten concluir que Pythium aphanidermatum es el causante de la Podredumbre basal de plantas adultas de Tomate. Sociedad Argentina de Botánica 2015-04-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original application/pdf https://revistas.unc.edu.ar/index.php/BSAB/article/view/10843 10.31055/1851.2372.v50.n1.10843 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 50 No. 1 (2015): Marzo; 11-15 Boletín de la Sociedad Argentina de Botánica; Vol. 50 Núm. 1 (2015): Marzo; 11-15 Boletín de la Sociedad Argentina de Botánica; v. 50 n. 1 (2015): Marzo; 11-15 1851-2372 0373-580X 10.31055/1851.2372.v50.n1 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/10843/11437
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-325
container_title_str Boletín de la Sociedad Argentina de Botánica
language Español
format Artículo revista
topic Oomycete
Pythium
Tomate
Oomycete
Pythium
Tomato
spellingShingle Oomycete
Pythium
Tomate
Oomycete
Pythium
Tomato
Grijalba, Pablo E.
Zapata, Raúl L.
Palmucci, Hemilse E
Baron, Claudio
Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
topic_facet Oomycete
Pythium
Tomate
Oomycete
Pythium
Tomato
author Grijalba, Pablo E.
Zapata, Raúl L.
Palmucci, Hemilse E
Baron, Claudio
author_facet Grijalba, Pablo E.
Zapata, Raúl L.
Palmucci, Hemilse E
Baron, Claudio
author_sort Grijalba, Pablo E.
title Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
title_short Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
title_full Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
title_fullStr Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
title_full_unstemmed Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota).
title_sort basal root rot of mature plants of tomato caused by pythium aphanidermatum (oomycota).
description Basal root rot of mature plants of tomato caused by Pythium aphanidermatum (Oomycota). In roots of adult tomato plants a wet and brown lesion, extending 2-4 cm above the soil was observed. Diseased plants withered and died. The objective of this paper was to isolate and to identify the causal organism of these symptoms. General and selective culture media were used. After 24 hours colonies with white cottony mycelium were developed. The morphology of the colony and the production, morphology and size of reproductive structures were evaluated. The rDNA extraction was made from mycelium of pure cultures 7-10 days old grown in agarized media. The ITS region was amplified using ITS4 and ITS5 primers, sequenced and compared in gene bank - NCBI BLAST server to verify their sequence similarity to the type or holotype available. Coenocytic mycelium, spherical-globular sporangium and aplerotic oospores, characteristic of the genus Pythium were observed in microscopic slides. Pathogenicity tests were conducted with positive results; a microorganism identical to the original was re-isolated. The characterization achieved by traditional techniques, molecular studies and the results of the pathogenicity tests support the conclusion that Pythium aphanidermatum is the causal agent of basal rot on mature plants of tomato
publisher Sociedad Argentina de Botánica
publishDate 2015
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/10843
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first_indexed 2024-09-03T22:48:18Z
last_indexed 2024-09-03T22:48:18Z
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