Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces

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The most commonly-used extraction methods for biological proxies are based on dividing each coprolite to analyze each proxy separately. However, the interpretation of results has limitations. The aim of the present work is to design a new multiproxy protocol for feces of South American camelids. The...

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Autores principales: Velázquez, Nadia Jimena, Petrigh, Romina, Benvenuto, María Laura, Martínez Tosto, Cecilia, Camiolo, Ivana, Palacio, Patricia, Fugassa, Martín Horacio, Valenzuela, Luciano Oscar, Burry, Lidia Susana
Formato: Artículo publishedVersion Artículo evaluado por pares
Lenguaje:Español
Publicado: Facultad de Filosofía y Letras, Universidad Nacional de Cuyo 2019
Materias:
Feces
Multiproxy
Lama guanicoe
multiproxy extraction protocol
Heces
Protocolo de extracción múltiple
Acceso en línea:http://revistas.uncu.edu.ar/ojs/index.php/analarqueyetno/article/view/3735
http://suquia.ffyh.unc.edu.ar/handle/suquia/11588
Aporte de:
Suquía - Instituto de Antropología de Córdoba (IDACOR, CONICET y UNC) de Universidad Nacional de Córdoba
id I10-R181-suquia-11588
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-181
collection Suquía - Instituto de Antropología de Córdoba (IDACOR, CONICET y UNC)
language Español
topic Feces
Multiproxy
Lama guanicoe
multiproxy extraction protocol
Heces
Multiproxy
Lama guanicoe
Protocolo de extracción múltiple
spellingShingle Feces
Multiproxy
Lama guanicoe
multiproxy extraction protocol
Heces
Multiproxy
Lama guanicoe
Protocolo de extracción múltiple
Velázquez, Nadia Jimena
Petrigh, Romina
Benvenuto, María Laura
Martínez Tosto, Cecilia
Camiolo, Ivana
Palacio, Patricia
Fugassa, Martín Horacio
Valenzuela, Luciano Oscar
Burry, Lidia Susana
Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
topic_facet Feces
Multiproxy
Lama guanicoe
multiproxy extraction protocol
Heces
Multiproxy
Lama guanicoe
Protocolo de extracción múltiple
description The most commonly-used extraction methods for biological proxies are based on dividing each coprolite to analyze each proxy separately. However, the interpretation of results has limitations. The aim of the present work is to design a new multiproxy protocol for feces of South American camelids. The protocol was based on reducing the use of chemical compounds in order to recover multiple proxies from a single sample. Theoretical and methodological considerations were taken into account to improve the protocol. The standard protocol was modified considering the requirements of multiproxy analysis. The experimental protocol was tested with a set of four guanaco (Lama guanicoe) feces from Patagonia, Argentina. After extraction, silica phytoliths, pollen types, and parasite remains were well-preserved and identified. Zoological origin was determined from DNA analysis. The results are discussed in light of various theoretical and methodological considerations. The implementation of this multiproxy extraction protocol in coprolites is an avenue for future research.
format Artículo
publishedVersion
Artículo evaluado por pares
author Velázquez, Nadia Jimena
Petrigh, Romina
Benvenuto, María Laura
Martínez Tosto, Cecilia
Camiolo, Ivana
Palacio, Patricia
Fugassa, Martín Horacio
Valenzuela, Luciano Oscar
Burry, Lidia Susana
author_facet Velázquez, Nadia Jimena
Petrigh, Romina
Benvenuto, María Laura
Martínez Tosto, Cecilia
Camiolo, Ivana
Palacio, Patricia
Fugassa, Martín Horacio
Valenzuela, Luciano Oscar
Burry, Lidia Susana
author_sort Velázquez, Nadia Jimena
title Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
title_short Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
title_full Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
title_fullStr Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
title_full_unstemmed Design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and DNA from Lama guanicoe feces
title_sort design and evaluation of a multiple extraction protocol for plant remains, silica phytoliths, pollen, parasites, stable isotopes and dna from lama guanicoe feces
publisher Facultad de Filosofía y Letras, Universidad Nacional de Cuyo
publishDate 2019
url http://revistas.uncu.edu.ar/ojs/index.php/analarqueyetno/article/view/3735
http://suquia.ffyh.unc.edu.ar/handle/suquia/11588
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