Sex differences in GABA-mediated calcium influx in hypothalamic neurons

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GABAA receptor (GABAAR) activation exerts trophic actions in immature neurons through depolarization of resting membrane potential gating the opening of voltage-dependent calcium channels. Previous results from our lab have shown gender-biased GABAAR responses in cultured hypothalamic neurons. These...

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Detalles Bibliográficos
Autores principales: Mir, Franco Rafael, Wilson, Carlos, Cambiasso, María Julia
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2022
Materias:
Calcio
Sex characteristics
Muscimol
Acceso en línea:http://hdl.handle.net/11086/24544
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R14111086-24544
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Calcio
Sex characteristics
Muscimol
spellingShingle Calcio
Sex characteristics
Muscimol
Mir, Franco Rafael
Wilson, Carlos
Cambiasso, María Julia
Sex differences in GABA-mediated calcium influx in hypothalamic neurons
topic_facet Calcio
Sex characteristics
Muscimol
description GABAA receptor (GABAAR) activation exerts trophic actions in immature neurons through depolarization of resting membrane potential gating the opening of voltage-dependent calcium channels. Previous results from our lab have shown gender-biased GABAAR responses in cultured hypothalamic neurons. These differences were found before brain masculinisation by gonadal hormones. Considering these, in this work we evaluated the GABAAR-mediated Ca2+ entry in cultured neurons segregated by gonadal type. Hypothalamic cells were obtained from embryonic brains at E16 (both male and female), two days before the peak of testosterone production by the foetal testis, and cultured for 2 days. To measure calcium signals, neurons were loaded with the calcium indicator Cal-520, followed by a time-lapse recording on live cells using a spinning disk microscope. Our results show that there are more male than female neurons responding to GABAAR stimulation. Additionally, almost 50% of male neurons did not recover basal calcium level after stimulation, in contrast to only 20% observed in females. Moreover, although nifedipine blocks intracellular calcium entry equally, it was stronger in males. Together, these results highlight the influence of neural sex differences irrespectively of sexual hormone exposure
format conferenceObject
author Mir, Franco Rafael
Wilson, Carlos
Cambiasso, María Julia
author_facet Mir, Franco Rafael
Wilson, Carlos
Cambiasso, María Julia
author_sort Mir, Franco Rafael
title Sex differences in GABA-mediated calcium influx in hypothalamic neurons
title_short Sex differences in GABA-mediated calcium influx in hypothalamic neurons
title_full Sex differences in GABA-mediated calcium influx in hypothalamic neurons
title_fullStr Sex differences in GABA-mediated calcium influx in hypothalamic neurons
title_full_unstemmed Sex differences in GABA-mediated calcium influx in hypothalamic neurons
title_sort sex differences in gaba-mediated calcium influx in hypothalamic neurons
publishDate 2022
url http://hdl.handle.net/11086/24544
work_keys_str_mv AT mirfrancorafael sexdifferencesingabamediatedcalciuminfluxinhypothalamicneurons
AT wilsoncarlos sexdifferencesingabamediatedcalciuminfluxinhypothalamicneurons
AT cambiassomariajulia sexdifferencesingabamediatedcalciuminfluxinhypothalamicneurons
bdutipo_str Repositorios
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