Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography

The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-de...

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Detalles Bibliográficos
Autores principales: Clop, P. D., Marchesini, G. R., Colpo, P., Perillo, M. A.
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2022
Materias:
Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
Acceso en línea:http://hdl.handle.net/11086/24368
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R14111086-24368
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
spellingShingle Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
Clop, P. D.
Marchesini, G. R.
Colpo, P.
Perillo, M. A.
Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
topic_facet Nanobiosensor
Beta-galactosidase
Surface plasmon resonance
Protein
description The present work was aimed at providing experimental support to the correlation between topographic and kinetic dimensions of protein function we described previously. Through electronic and colloidal nanolithographic techniques we produced surfaces exhibiting a fractal-like pattern with a pre-determined topographic dimension of domains capable to bind proteins in a covalent manner (AbAntiβ -Gal). These surfaces were used as sensors to reversibly bind β-Gal and enabled the kinetic study of AbAnti β-Gal -β-Gal complex formation by surface plasmon resonance (SPR) spectroscopy. Compared with the behavior of control sensors (homogeneous topography), the Ag-Ab binding kinetics in sensors produced by nanolithography showed higher capacity and broader dispersion of binding sites characterized by a more diffuse attractor in the kd vs. Kd phase space
format conferenceObject
author Clop, P. D.
Marchesini, G. R.
Colpo, P.
Perillo, M. A.
author_facet Clop, P. D.
Marchesini, G. R.
Colpo, P.
Perillo, M. A.
author_sort Clop, P. D.
title Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_short Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_full Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_fullStr Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_full_unstemmed Binding kinetics between soluble beta-Gal and anti-beta-Gal immobilized on chemically nanopatterned surfaces with fractal topography
title_sort binding kinetics between soluble beta-gal and anti-beta-gal immobilized on chemically nanopatterned surfaces with fractal topography
publishDate 2022
url http://hdl.handle.net/11086/24368
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