P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa

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Objective: Chronic mechanical irritation (CMI) has been proposed as risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p1...

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Autores principales: Lazos, Jerónimo, Piemonte, Eduardo David, Brunotto, Mabel
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2022
Materias:
Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
Acceso en línea:http://hdl.handle.net/11086/23672
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R14111086-23672
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
spellingShingle Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
Lazos, Jerónimo
Piemonte, Eduardo David
Brunotto, Mabel
P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
topic_facet Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
description Objective: Chronic mechanical irritation (CMI) has been proposed as risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT methylation in a specific CMI lesion: Chronic Traumatic Ulcer (CTU). Study Design: A split-mouth design was used, and two samples per individual were taken using cytobrush: CTU lesion (according to Piemonte et al. modified criteria) and clinically normal mucosa of a contralateral site. After extracting DNA, p16 and MGMT methylation status was assessed using qPCR.Results: 27 patients were studied, mean age 59.1. The most frequent CMI factor was Functional (70%), and CMI evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a statistically significant difference (p<0,0001 and p <0,0005, respectively) when compared to control site.Conclusion: CMI affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced. This suggests that CMI could foster carcinogenesis through epigenetic alterations.
format conferenceObject
author Lazos, Jerónimo
Piemonte, Eduardo David
Brunotto, Mabel
author_facet Lazos, Jerónimo
Piemonte, Eduardo David
Brunotto, Mabel
author_sort Lazos, Jerónimo
title P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_short P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_full P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_fullStr P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_full_unstemmed P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_sort p16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
publishDate 2022
url http://hdl.handle.net/11086/23672
work_keys_str_mv AT lazosjeronimo p16andmgmtmethylationinchronicmechanicalirritationoftheoralmucosa
AT piemonteeduardodavid p16andmgmtmethylationinchronicmechanicalirritationoftheoralmucosa
AT brunottomabel p16andmgmtmethylationinchronicmechanicalirritationoftheoralmucosa
bdutipo_str Repositorios
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