Reproductive performance of male mice after hypothalamic ghrelin administration
12 p.
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| Formato: | publishedVersion article |
| Lenguaje: | Inglés |
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2023
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| Acceso en línea: | http://hdl.handle.net/11086/549245 |
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I10-R141-11086-549245 |
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dspace |
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Universidad Nacional de Córdoba |
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I-10 |
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R-141 |
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Repositorio Digital Universitario (UNC) |
| language |
Inglés |
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Reproduction Gherelin Spermatozoa Testosterone |
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Reproduction Gherelin Spermatozoa Testosterone Poretti, María Belén Frautschi, Camila Luque, Eugenia Mercedes Bianconi, Santiago Martini, Ana Carolina Stutz, Graciela Vincenti, Laura María Santillán, María Emilia Ponzio, Marina Flavia Schiöth, Helgi Fiol De Cuneo, Marta Haydee Bianconi, Santiago Carlini, Valeria Paola Reproductive performance of male mice after hypothalamic ghrelin administration |
| topic_facet |
Reproduction Gherelin Spermatozoa Testosterone |
| description |
12 p. |
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https://orcid.org/0000-0002-4742-2986 |
| author_facet |
https://orcid.org/0000-0002-4742-2986 Poretti, María Belén Frautschi, Camila Luque, Eugenia Mercedes Bianconi, Santiago Martini, Ana Carolina Stutz, Graciela Vincenti, Laura María Santillán, María Emilia Ponzio, Marina Flavia Schiöth, Helgi Fiol De Cuneo, Marta Haydee Bianconi, Santiago Carlini, Valeria Paola |
| format |
publishedVersion article |
| author |
Poretti, María Belén Frautschi, Camila Luque, Eugenia Mercedes Bianconi, Santiago Martini, Ana Carolina Stutz, Graciela Vincenti, Laura María Santillán, María Emilia Ponzio, Marina Flavia Schiöth, Helgi Fiol De Cuneo, Marta Haydee Bianconi, Santiago Carlini, Valeria Paola |
| author_sort |
Poretti, María Belén |
| title |
Reproductive performance of male mice after hypothalamic ghrelin administration |
| title_short |
Reproductive performance of male mice after hypothalamic ghrelin administration |
| title_full |
Reproductive performance of male mice after hypothalamic ghrelin administration |
| title_fullStr |
Reproductive performance of male mice after hypothalamic ghrelin administration |
| title_full_unstemmed |
Reproductive performance of male mice after hypothalamic ghrelin administration |
| title_sort |
reproductive performance of male mice after hypothalamic ghrelin administration |
| publishDate |
2023 |
| url |
http://hdl.handle.net/11086/549245 |
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1806949371068022784 |
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I10-R141-11086-5492452023-12-13T11:52:33Z Reproductive performance of male mice after hypothalamic ghrelin administration Poretti, María Belén Frautschi, Camila Luque, Eugenia Mercedes Bianconi, Santiago Martini, Ana Carolina Stutz, Graciela Vincenti, Laura María Santillán, María Emilia Ponzio, Marina Flavia Schiöth, Helgi Fiol De Cuneo, Marta Haydee Bianconi, Santiago Carlini, Valeria Paola https://orcid.org/0000-0002-4742-2986 https://orcid.org/0000-0001-7656-7212 https://orcid.org/0000-0002-1116-1497 https://orcid.org/0000-0001-7414-3942 https://orcid.org/0000-0002-3549-2063 https://orcid.org/0000-0002-4102-6990 Reproduction Gherelin Spermatozoa Testosterone 12 p. info:eu-repo/semantics/publishedVersion Fil: Porretti, María Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Porretti, María Belén. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Frautschi, Camila. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Luque, Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Bianconi, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Bianconi, Santiago. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Martini, Ana Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Vicenti, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Santillán, María Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Ponzio, Marina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Schiöth, Helgi B. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Fiol de Cuneo, Marta. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina Fil: Carlini, Valeria Paola. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Carlini, Valeria Paola. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (GHRL) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor that is being investigated for its potential role on reproduction. Considering that data available so far are still limited and characterization of GHRL action mechanism on the reproductive system has not been fully elucidated, we studied the participation of hypothalamus in GHRL effects on sperm functional activity, plasma levels of gonadotropins and histological morphology in mice testes after hypothalamic infusion of 0.3 or 3.0 nmol/day GHRL or artificial cerebrospinal fluid (ACSF) at different treatment periods. We found that GHRL 3.0 nmol/day administration for 42 days significantly reduced sperm concentration (GHRL 3.0 nmol/day = 14.05 ± 2.44 × 106/mL vs ACSF = 20.33 ± 1.35 × 106/mL, P< 0.05) and motility (GHRL 3.0 nmol/day = 59.40 ± 4.20% vs ACSF = 75.80 ± 1.40%, P< 0.05). In addition, histological studies showed a significant decrease percentage of spermatogonia (GHRL 3.0 nmol/day = 6.76 ± 0.68% vs ACSF = 9.56 ± 0.41%, P< 0.05) and sperm (GHRL 3.0 nmol/day = 24.24 ± 1.92% vs ACSF = 31.20 ± 3.06%, P< 0.05). These results were associated with a significant reduction in luteinizing hormone and testosterone plasma levels (P< 0.05). As GHRL is an orexigenic peptide, body weight and food intake were measured. Results showed that GHRL increases both parameters; however, the effect did not last beyond the first week of treatment. Results presented in this work confirm that central GHRL administration impairs spermatogenesis and suggest that this effect is mediated by inhibition of hypothalamic–pituitary–gonadal axis. http://www.reproduction-online.org/lookup/doi/10.1530/REP-17-0535 info:eu-repo/semantics/publishedVersion Fil: Porretti, María Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Porretti, María Belén. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Frautschi, Camila. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Luque, Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Bianconi, Santiago. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Bianconi, Santiago. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Martini, Ana Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Vicenti, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Santillán, María Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina. Fil: Ponzio, Marina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Schiöth, Helgi B. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Fil: Fiol de Cuneo, Marta. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Fisiología Humana; Argentina Fil: Carlini, Valeria Paola. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Carlini, Valeria Paola. Uppsala University. Departament of Neuroscience. Functional Pharmacology; Sweden. Otras Ciencias de la Salud 2023-09-29T20:08:56Z 2023-09-29T20:08:56Z 2018 article 1470–1626 http://hdl.handle.net/11086/549245 1741–7899 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso; Electrónico y/o Digital |