Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress

Fil: Lescano, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.

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Autores principales: Lescano, Ignacio, Martini, Carolina, Tessi, Tomás, González, Claudio, Desimone, Marcelo
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2022
Materias:
Acceso en línea:http://hdl.handle.net/11086/29461
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id I10-R141-11086-29461
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Allantoin
Allantoinase regulation
Salt stress
Nitrogen
spellingShingle Allantoin
Allantoinase regulation
Salt stress
Nitrogen
Lescano, Ignacio
Martini, Carolina
Tessi, Tomás
González, Claudio
Desimone, Marcelo
Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
topic_facet Allantoin
Allantoinase regulation
Salt stress
Nitrogen
description Fil: Lescano, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
format conferenceObject
author Lescano, Ignacio
Martini, Carolina
Tessi, Tomás
González, Claudio
Desimone, Marcelo
author_facet Lescano, Ignacio
Martini, Carolina
Tessi, Tomás
González, Claudio
Desimone, Marcelo
author_sort Lescano, Ignacio
title Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
title_short Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
title_full Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
title_fullStr Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
title_full_unstemmed Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress
title_sort ureide synthesis, accumulation and transport in arabidopsis plants under salt and osmotic stress
publishDate 2022
url http://hdl.handle.net/11086/29461
work_keys_str_mv AT lescanoignacio ureidesynthesisaccumulationandtransportinarabidopsisplantsundersaltandosmoticstress
AT martinicarolina ureidesynthesisaccumulationandtransportinarabidopsisplantsundersaltandosmoticstress
AT tessitomas ureidesynthesisaccumulationandtransportinarabidopsisplantsundersaltandosmoticstress
AT gonzalezclaudio ureidesynthesisaccumulationandtransportinarabidopsisplantsundersaltandosmoticstress
AT desimonemarcelo ureidesynthesisaccumulationandtransportinarabidopsisplantsundersaltandosmoticstress
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spelling I10-R141-11086-294612023-08-30T13:57:00Z Ureide synthesis, accumulation and transport in Arabidopsis plants under salt and osmotic stress Lescano, Ignacio Martini, Carolina Tessi, Tomás González, Claudio Desimone, Marcelo Allantoin Allantoinase regulation Salt stress Nitrogen Fil: Lescano, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Lescano, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Martini, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Martini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Tessi, Tomás. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Tessi, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: González, Claudio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: González, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Desimone, Marcelo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. The ureides allantoin and allantoic acid play a central role in nitrogen transport in nodulating tropical legumes. However, the complete enzyme set for ureide synthesis and a family of ureide permeases are widely distributed in the plant kingdom suggesting their participation in physiological processes not properly characterized yet. In Arabidopsis, microarrays studies showed an upregulation of ureides synthesis genes (xanthine dehydrogenase, uricase) during abiotic stresses. On the contrary, allantoinase gene expression is strongly reduced after stress suggesting that allantoin may accumulate in the cells. We observed accumulation of allantoin in Arabidopsis plants under osmotic and salt stresses. This effect was exacerbated in plants grown with ammonium as nitrogen source and suppressed in the presence of sucrose as carbon source. The analysis of two independent T-DNA insertion lines, causing knockout of allantoinase (alla-1 and alla-2) showed constitutively elevated concentrations of allantoin, but a noticeable morphological phenotype remained elusive. To determine the physiological relevance of allantoinase gene repression on resistance to stress, transgenic lines were generated on the genotype alla-1, in which the coding sequence of allantoinase was introduced under the control of the stress inducible promoter RD29A. As expected, alla-1 pRD29A::Aln plants were not able to accumulate allantoin under stress conditions. The phenotype of KO and RD29A:ALN plants was analysed under salt and osmotic stress conditions. In addition, the phenotype of a KO mutant of AtUPS5 (ups5) was analysed. AtUPS5 transports allantoin, is expressed in the root cortex and endodermis and its expression increases during salt and osmotic stress, suggesting a rol in long-distance transport of allantoin during stress. Fil: Lescano, Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Lescano, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Martini, Carolina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Martini, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Tessi, Tomás. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Tessi, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: González, Claudio. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: González, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Fil: Desimone, Marcelo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Fil: Desimone, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina. Bioquímica y Biología Molecular (ídem 3.1.10) 2022-11-08T12:23:39Z 2022-11-08T12:23:39Z 2013 conferenceObject http://hdl.handle.net/11086/29461 eng Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso; Electrónico y/o Digital