The thermal unfolding of beta 2-Glycoprotein

Fil: Bazan, Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina.

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Detalles Bibliográficos
Autores principales: Bazan, Soledad, Paolorossi, Mariana, Montich, Guillermo
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2022
Materias:
Differential scanning calorimetry
Circular dichroism
B2GPI
Human plasma
Blood coagulation
Acceso en línea:http://hdl.handle.net/11086/28231
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R141-11086-28231
record_format dspace
spelling I10-R141-11086-282312023-08-30T13:57:38Z The thermal unfolding of beta 2-Glycoprotein Bazan, Soledad Paolorossi, Mariana Montich, Guillermo Differential scanning calorimetry Circular dichroism B2GPI Human plasma Blood coagulation Fil: Bazan, Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Fil: Paolorossi, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Fil: Montich, Guillermo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Beta 2- Glycoprotein I (B2GPI) is an abundant glycoprotein of human plasma. It participates in blood coagulation processes and the clearance of phosphatidylserine exposing- apoptotic cells. B2GPI is also the major antigen involved in the aberrant immune response that characterizes the antiphospholipid syndrome. B2GPI consists of a single chain of 326 aminoacids arranged in five domains. Four of them are the so called sushi or short consensus repeat (SCR) domain with its conserved beta sandwich- arrangement. The fifth domain is larger and it has a lysine rich region that was shown to participate in the interaction of B2GPI with anionic lipids. B2GPI has four N-glycosilation sites with complex bi and triantennary complex glycans containing sialic acid that comprise ~20% of its total weight. To study the folding behavior of this multiple domain glycoprotein we performed differential scanning calorimetry at various pH and salts conditions. We found that at pH 7 the protein unfolding proceeds with a pre-transition at lower temperatures. This effect is more pronounced in the absence of salt. In order to characterize this earlier intermediate we perfomed circular dichroism studies of the thermal unfolding of B2GPI in the far UV region. The transition followed by the far UV signal shows a two state unfolding transition centered at the same temperature of the calorimetric one. This means that the secondary structure of the protein unfolds in a cooperative manner. Fil: Bazan, Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Fil: Paolorossi, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Fil: Montich, Guillermo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Otras Ciencias Químicas 2022-08-18T15:30:01Z 2022-08-18T15:30:01Z 2013 conferenceObject 978-987-27591-2-4 http://hdl.handle.net/11086/28231 eng Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Differential scanning calorimetry
Circular dichroism
B2GPI
Human plasma
Blood coagulation
spellingShingle Differential scanning calorimetry
Circular dichroism
B2GPI
Human plasma
Blood coagulation
Bazan, Soledad
Paolorossi, Mariana
Montich, Guillermo
The thermal unfolding of beta 2-Glycoprotein
topic_facet Differential scanning calorimetry
Circular dichroism
B2GPI
Human plasma
Blood coagulation
description Fil: Bazan, Soledad. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina.
format conferenceObject
author Bazan, Soledad
Paolorossi, Mariana
Montich, Guillermo
author_facet Bazan, Soledad
Paolorossi, Mariana
Montich, Guillermo
author_sort Bazan, Soledad
title The thermal unfolding of beta 2-Glycoprotein
title_short The thermal unfolding of beta 2-Glycoprotein
title_full The thermal unfolding of beta 2-Glycoprotein
title_fullStr The thermal unfolding of beta 2-Glycoprotein
title_full_unstemmed The thermal unfolding of beta 2-Glycoprotein
title_sort thermal unfolding of beta 2-glycoprotein
publishDate 2022
url http://hdl.handle.net/11086/28231
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AT paolorossimariana thermalunfoldingofbeta2glycoprotein
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