Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s

The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene...

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Detalles Bibliográficos
Autores principales: Clop, Eduardo Matias, Chattah, Ana Karina, Perillo, María Angélica
Formato: article
Lenguaje:Inglés
Publicado: 2022
Materias:
Lipid vesicles
Peg
Pe-Peg
Nuclear magnetic resonance
NMR
Water Dynamics
Acceso en línea:http://hdl.handle.net/11086/28112
http://dx.doi.org/10.1021/jp410894x
http://dx.doi.org/10.1021/jp410894x
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R141-11086-28112
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic Lipid vesicles
Peg
Pe-Peg
Nuclear magnetic resonance
NMR
Water Dynamics
spellingShingle Lipid vesicles
Peg
Pe-Peg
Nuclear magnetic resonance
NMR
Water Dynamics
Clop, Eduardo Matias
Chattah, Ana Karina
Perillo, María Angélica
Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
topic_facet Lipid vesicles
Peg
Pe-Peg
Nuclear magnetic resonance
NMR
Water Dynamics
description The present work was aimed at studying the molecular dynamics at different levels of model membranes having a simulated glycoclix, with focus on the molecular crowding conditions at the lipid–water interfacial region. Thus, binary mixtures of dipalmitoylphosphatidylcholine (dpPC) and a poly(ethylene glycol) (PEGn) derivative of dipalmitoylphosphatidylethanolamine (PE) (where n = 350, 1000, and 5000, respectively, refer to PEG molecular masses) were submitted to 1H spin–lattice relaxation time (T1) and 31P NMR spectra analysis. 1H NMR relaxation times revealed two contributing components in each proton system (PEG, phospholipids, and water), for all the mixtures studied, exhibiting values of T1 with very different orders of magnitude. This allowed identifying confined and bulk water populations as well as PEG moieties becoming more disordered and independent from the phospholipid moiety as n increased. 31P spectra showed a typical broad bilayer signal for n = 350 and 1000, and an isotropic signal characteristic of micelles for n = 5000. Surface pressure (π)–molecular area isotherms and compressional modulus measurements provided further structural information. Moreover, epifluorescence microscopy data from monolayers at π ∼ 30 mN/m, the expected equilibrium π in lipid bilayers, allowed us to postulate that both 1H populations resolved through NMR in phospholipids and lipopolymers corresponded to different phase domains.
format article
author Clop, Eduardo Matias
Chattah, Ana Karina
Perillo, María Angélica
author_facet Clop, Eduardo Matias
Chattah, Ana Karina
Perillo, María Angélica
author_sort Clop, Eduardo Matias
title Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
title_short Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
title_full Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
title_fullStr Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
title_full_unstemmed Water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
title_sort water and membrane dynamics in suspensions of lipid vesicles functionalized with poly(ethylene glycol)s
publishDate 2022
url http://hdl.handle.net/11086/28112
http://dx.doi.org/10.1021/jp410894x
http://dx.doi.org/10.1021/jp410894x
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AT chattahanakarina waterandmembranedynamicsinsuspensionsoflipidvesiclesfunctionalizedwithpolyethyleneglycols
AT perillomariaangelica waterandmembranedynamicsinsuspensionsoflipidvesiclesfunctionalizedwithpolyethyleneglycols
bdutipo_str Repositorios
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