An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements

Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenas...

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Autor principal: Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A.
Formato: article
Lenguaje:Inglés
Publicado: 2019
Materias:
GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS
Acceso en línea:http://dx.doi.org/10.3290/j.jad.a29588
http://hdl.handle.net/11086/13456
Aporte de:
Repositorio Digital Universitario (UNC) de Universidad Nacional de Córdoba
id I10-R141-11086-13456
record_format dspace
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-141
collection Repositorio Digital Universitario (UNC)
language Inglés
topic GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS
spellingShingle GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS
Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A.
An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
topic_facet GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS
description Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts.
format article
author Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A.
author_facet Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A.
author_sort Rodríguez, IA; Rozas, CA.; Campos-Sánchez, F.; Alaminos Mignorance, M.; Uribe Echevarria, J.; Campos A.
title An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
title_short An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
title_full An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
title_fullStr An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
title_full_unstemmed An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
title_sort in vitro biocompatibility study of conventional and resin-modified glass ionomer cements
publishDate 2019
url http://dx.doi.org/10.3290/j.jad.a29588
http://hdl.handle.net/11086/13456
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