Cryopreservation of an artificial human oral mucosa stroma. A viability
<font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1"> <p align="LEFT">The aim of this study was to evaluate the viability and biomechanical properties of artificial human oral</p> <p align="L...
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| Formato: | article |
| Lenguaje: | Inglés |
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2019
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| Acceso en línea: | http://hdl.handle.net/11086/13452 |
| Aporte de: |
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I10-R141-11086-13452 |
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| record_format |
dspace |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-141 |
| collection |
Repositorio Digital Universitario (UNC) |
| language |
Inglés |
| topic |
CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX |
| spellingShingle |
CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez Cryopreservation of an artificial human oral mucosa stroma. A viability |
| topic_facet |
CRYOPRESERVATION; TISSUE ENGINEERING; HUMAN GINGIVAL FIBROBLASTS; FIBRIN?AGAROSE MATRIX |
| description |
<font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">
<p align="LEFT">The aim of this study was to evaluate the viability and biomechanical properties of artificial human oral</p>
<p align="LEFT">mucosa stroma (HOMS) subjected to cryopreservation with different cryoprotectant solutions.</p>
<p align="LEFT">Artificial HOMS based on a fibrin?agarose matrix with human gingival fibroblasts cultured 7 days</p>
<p align="LEFT">in vitro were cryopreserved with three cryoprotectant solutions: (A) TC-199 Medium, DMSO 15%, albumin;</p>
<p align="LEFT">(B) DMEM, FCS, DMSO 10%; (C) QC Medium, glycerol. As controls, artificial HOMS not subjected to</p>
<p align="LEFT">cryopreservation (CF) and HOMS cryopreserved without cryoprotectant solution (CS) were used. Histological</p>
<p align="LEFT">analysis by light microscopy showed that solutions A and B preserved a pattern of porosity similar</p>
<p align="LEFT">to values in CF. Based on the number of intact cells in the fibrin?agarose matrix, substitutes preserved</p>
<p align="LEFT">with solution B showed the best results. Cell proliferation detected with PCNA immunochemical methods</p>
<p align="LEFT">showed that the cell proliferation index was highest in substitutes cryopreserved with solution B. The</p></font></font>
<p align="LEFT"><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">reculture method and cell viability analyses with Live & Dead</font></font><font face="AdvPSSym" size="1"><font face="AdvPSSym" size="1"> </font></font><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">revealed increased number of viable in</font></font></p><font face="AdvGulliv-R" size="1"><font face="AdvGulliv-R" size="1">
<p align="LEFT">cells preserved with solution B. Artificial stroma substitutes in CS control samples showed the greatest</p>
<p align="LEFT">alterations in microstructure and cell proliferation. Analysis of the biomechanical properties showed that</p>
<p align="LEFT">substitutes cryopreserved with different solutions had adequate rheological parameters (yield stress,</p>
<p align="LEFT">elastic modulus and viscous modulus) and were therefore suitable for use in regenerative medicine.</p>
<p align="LEFT">These results establish effective methods of cryopreservation for all experimental situations and suggest</p>
<p align="LEFT">that solution B (DMEM, FCS, DMSO 10%) was the best cryoprotectant for the cryopreservation of an artificial</p>
<p>oral human mucosa substitute based on a fibrin?agarose matrix.</p></font></font> |
| format |
article |
| author |
Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| author_facet |
Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| author_sort |
Mario A. Rodriguez; Modesto T. López-López; Juan D.G. Durán; Miguel Alaminos; Antonio Campos; Ismael A. Rodriguez |
| title |
Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_short |
Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_full |
Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_fullStr |
Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_full_unstemmed |
Cryopreservation of an artificial human oral mucosa stroma. A viability |
| title_sort |
cryopreservation of an artificial human oral mucosa stroma. a viability |
| publishDate |
2019 |
| url |
http://hdl.handle.net/11086/13452 |
| work_keys_str_mv |
AT marioarodriguezmodestotlopezlopezjuandgduranmiguelalaminosantoniocamposismaelarodriguez cryopreservationofanartificialhumanoralmucosastromaaviability |
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Repositorios |
| _version_ |
1764820391109328902 |