Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart

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Background and aims: One of the few tree species adapted to the ecologically limiting conditions of the Atacama Desert is Geoffroea decorticans, known as chañar. It is a valuable multipurpose resource used as a food and medicinal product. However, for the purpose of genetic studies, codominant DNA m...

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Autores principales: Contreras Diaz, Roberto César, Carevic Vergara, Felipe, Arias Aburto, Mariana, Huanca Mamani, Wilson, Díaz Martín, Bladimir
Formato: Artículo revista
Lenguaje:Español
Publicado: Sociedad Argentina de Botánica 2021
Materias:
Desierto de Atacama
diversidad genética
Geoffroea decorticans
marcadores SSR
next-generation sequencing
Atacama desert
genetic diversity
SSR markers
Acceso en línea:https://revistas.unc.edu.ar/index.php/BSAB/article/view/32721
Aporte de:
Revistas de la UNC de Universidad Nacional de Córdoba
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institution Universidad Nacional de Córdoba
institution_str I-10
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container_title_str Revistas de la UNC
language Español
format Artículo revista
topic Desierto de Atacama
diversidad genética
Geoffroea decorticans
marcadores SSR
next-generation sequencing
Atacama desert
genetic diversity
Geoffroea decorticans
next-generation sequencing
SSR markers
spellingShingle Desierto de Atacama
diversidad genética
Geoffroea decorticans
marcadores SSR
next-generation sequencing
Atacama desert
genetic diversity
Geoffroea decorticans
next-generation sequencing
SSR markers
Contreras Diaz, Roberto César
Carevic Vergara, Felipe
Arias Aburto, Mariana
Huanca Mamani, Wilson
Díaz Martín, Bladimir
Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
topic_facet Desierto de Atacama
diversidad genética
Geoffroea decorticans
marcadores SSR
next-generation sequencing
Atacama desert
genetic diversity
Geoffroea decorticans
next-generation sequencing
SSR markers
author Contreras Diaz, Roberto César
Carevic Vergara, Felipe
Arias Aburto, Mariana
Huanca Mamani, Wilson
Díaz Martín, Bladimir
author_facet Contreras Diaz, Roberto César
Carevic Vergara, Felipe
Arias Aburto, Mariana
Huanca Mamani, Wilson
Díaz Martín, Bladimir
author_sort Contreras Diaz, Roberto César
title Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
title_short Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
title_full Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
title_fullStr Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
title_full_unstemmed Development of new microsatelite loci for Geoffroea decorticans (Gillies ex Hook. & Arn.) Burkart
title_sort development of new microsatelite loci for geoffroea decorticans (gillies ex hook. & arn.) burkart
description Background and aims: One of the few tree species adapted to the ecologically limiting conditions of the Atacama Desert is Geoffroea decorticans, known as chañar. It is a valuable multipurpose resource used as a food and medicinal product. However, for the purpose of genetic studies, codominant DNA markers specific for this species have not yet been developed. The objective of this work is to develop and validate microsatellite markers (SSR) for G. decorticans, in order to perform future studies of genetic diversity and genetic structure of populations. M&M: SSR markers were searched in the G. decorticans genome using the Next-Generation Sequencing (NGS) method, and they were validated through thirty individuals distributed in different localities in northern Chile. Results: A total of ~ 144,117 microsatellite loci were identified and a set of 41 primer pairs was used for validation. The amplified fragments ranged from 106 bp to 225 bp, the number of alleles ranged from 2 to 9, and the PIC value of the 41 SSR loci ranged between 0.32 and 0.86, with an average of 0.64. Conclusions: For the first time, putative neutral SSR markers specific to the G. decorticans species have been developed in order to promote genetic studies for the conservation of the species. The present study provides a set of 38 new polymorphic SSR markers, which could serve as an effective tool to estimate genetic diversity, genetic structure and to be used in breeding programs.
publisher Sociedad Argentina de Botánica
publishDate 2021
url https://revistas.unc.edu.ar/index.php/BSAB/article/view/32721
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