In vitro establishment of four olive varieties grown in the field (Olea europea L.)

Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the expla...

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Autores principales: Turina, C. A., Bima, P.
Formato: Artículo revista
Lenguaje:Español
Publicado: Facultad de Ciencias Agropecuarias. Área de Difusión Científica 2017
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Acceso en línea:https://revistas.unc.edu.ar/index.php/agris/article/view/19041
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id I10-R10article-19041
record_format ojs
institution Universidad Nacional de Córdoba
institution_str I-10
repository_str R-10
container_title_str Revistas de la UNC
language Español
format Artículo revista
topic ultrasonido
zeatina
6-benciladenina
manitol
carbón activado
ultrasound
zeatin
6-benzyladenine
mannitol
activated charcoal.
spellingShingle ultrasonido
zeatina
6-benciladenina
manitol
carbón activado
ultrasound
zeatin
6-benzyladenine
mannitol
activated charcoal.
Turina, C. A.
Bima, P.
In vitro establishment of four olive varieties grown in the field (Olea europea L.)
topic_facet ultrasonido
zeatina
6-benciladenina
manitol
carbón activado
ultrasound
zeatin
6-benzyladenine
mannitol
activated charcoal.
author Turina, C. A.
Bima, P.
author_facet Turina, C. A.
Bima, P.
author_sort Turina, C. A.
title In vitro establishment of four olive varieties grown in the field (Olea europea L.)
title_short In vitro establishment of four olive varieties grown in the field (Olea europea L.)
title_full In vitro establishment of four olive varieties grown in the field (Olea europea L.)
title_fullStr In vitro establishment of four olive varieties grown in the field (Olea europea L.)
title_full_unstemmed In vitro establishment of four olive varieties grown in the field (Olea europea L.)
title_sort in vitro establishment of four olive varieties grown in the field (olea europea l.)
description Plant sanitation through in vitro culture helps addressing sanitary issues that affect olive production (Olea europaea L.). Our goal was to develop a protocol for in vitro establishment of plant material obtained from adult plants grown in the field. We tested four methods of disinfection, the explants response to two cytokinins (BA, zeatin), two carbon sources (sucrose, mannitol), and activated charcoal (0 y 1 g.L-1), and the impact of the season in which materials were collected. The use of ultrasound during disinfection allowed satisfactory establishment rates (44 %). BA improved sprouting (36 % vs. 24 %), and so did mannitol (75 % vs. 20 %). The addition of activated charcoal did not show statistically significant effects on both survival and sprouting. We observed variations depending on the season of collection and the plant variety. Spring was –for all varieties– the most favourable time to collect explants: the highest sprouting rates (21 % to 62 %) and the lowest contamination levels were obtained, Frantoio and Manzanilla being the varieties least affected by contamination. 
publisher Facultad de Ciencias Agropecuarias. Área de Difusión Científica
publishDate 2017
url https://revistas.unc.edu.ar/index.php/agris/article/view/19041
work_keys_str_mv AT turinaca invitroestablishmentoffourolivevarietiesgrowninthefieldoleaeuropeal
AT bimap invitroestablishmentoffourolivevarietiesgrowninthefieldoleaeuropeal
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