Comparison of two experimental models of glaucoma in rabbits
Objective: to compare two models of experimental glaucoma by induction of ocular hypertension in rabbits.Materials and methods: Sixteen New Zealand female rabbits, 2-3 kg were used. Model A (n=6): cauterization of episcleral and perilimbar veins of the right eye (RE) with surgical electrocautery. Mo...
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| Formato: | Artículo revista |
| Lenguaje: | Español Inglés |
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Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología
2018
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/med/article/view/16811 |
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I10-R10-article-16811 |
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ojs |
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Universidad Nacional de Córdoba |
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I-10 |
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R-10 |
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Revistas de la UNC |
| language |
Español Inglés |
| format |
Artículo revista |
| topic |
experimental glaucoma model ?-chymotrypsin cauterization of episcleral veins ocular hypertension Modelo experimental de glaucoma ?-quimotripsina cauterización de las venas epiesclerales hipertensión ocular |
| spellingShingle |
experimental glaucoma model ?-chymotrypsin cauterization of episcleral veins ocular hypertension Modelo experimental de glaucoma ?-quimotripsina cauterización de las venas epiesclerales hipertensión ocular Tartara, Luis Ignacio Leavi, Stefania Campana, Vilma Allemandi, Daniel Palma, Santiago Comparison of two experimental models of glaucoma in rabbits |
| topic_facet |
experimental glaucoma model ?-chymotrypsin cauterization of episcleral veins ocular hypertension Modelo experimental de glaucoma ?-quimotripsina cauterización de las venas epiesclerales hipertensión ocular |
| author |
Tartara, Luis Ignacio Leavi, Stefania Campana, Vilma Allemandi, Daniel Palma, Santiago |
| author_facet |
Tartara, Luis Ignacio Leavi, Stefania Campana, Vilma Allemandi, Daniel Palma, Santiago |
| author_sort |
Tartara, Luis Ignacio |
| title |
Comparison of two experimental models of glaucoma in rabbits |
| title_short |
Comparison of two experimental models of glaucoma in rabbits |
| title_full |
Comparison of two experimental models of glaucoma in rabbits |
| title_fullStr |
Comparison of two experimental models of glaucoma in rabbits |
| title_full_unstemmed |
Comparison of two experimental models of glaucoma in rabbits |
| title_sort |
comparison of two experimental models of glaucoma in rabbits |
| description |
Objective: to compare two models of experimental glaucoma by induction of ocular hypertension in rabbits.Materials and methods: Sixteen New Zealand female rabbits, 2-3 kg were used. Model A (n=6): cauterization of episcleral and perilimbar veins of the right eye (RE) with surgical electrocautery. Model B (n=10): Injection of ?-chymotrypsin in posterior chamber of RE. Intraocular pressure (IOP) was measured before and after the induction of ocular hypertension (OHT), once a week at the same time of day for 40 days, with a manual tonometer. The animals were euthanized by CO2 inhalation. In both models the control was the IOP of the left eye (LE). The mean and standard error (SE) values of IOP, expressed in mmHg, were compared statistically by applying Student's t-test with a significance level of p<0.05. Results: The IOP in LE (control) of model A: was 12.9±1.05 and in model B: 12.9±1.09. There were no significant differences between the models. Model A: The IOP increase in RE was 14.7% (14.8±1.4) with respect to LE. A significant increase in IOP was observed within the first 24 hours: 23.5±1.9 (p<0.05) compared to the control eye. There were no significant differences with subsequent controls. Model B: The increase in IOP in RE was 129.1% (29.6±3.4) with respect to LE. In all cases an increase was observed from Day 1 (p<0.05). The IOP peak in RE was evidenced on Day 25: 35±3.4 (p<0.05). The increase in IOP induced by model B was significantly higher (p<0.01) than in model A. There was loss of ganglion cells of the retina in both models, but the following anatomo-pathological changes were observed only in model B: buphthalmos, subluxation of the lens and increased excavation of the papilla. Conclusion This study indicates that model B is the most appropriate method to induce a rapid, controlled increase of IOP in rabbits and, more importantly, that this increase may be sustained over extended periods of time. This model could be useful for evaluating the efficacy of new ocular drug delivery systems and for further studies of the physiopathology of glaucoma. |
| publisher |
Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología |
| publishDate |
2018 |
| url |
https://revistas.unc.edu.ar/index.php/med/article/view/16811 |
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I10-R10-article-168112019-05-20T10:54:31Z Comparison of two experimental models of glaucoma in rabbits Comparación de dos modelos experimentales de glaucoma en conejos Tartara, Luis Ignacio Leavi, Stefania Campana, Vilma Allemandi, Daniel Palma, Santiago experimental glaucoma model ?-chymotrypsin cauterization of episcleral veins ocular hypertension Modelo experimental de glaucoma ?-quimotripsina cauterización de las venas epiesclerales hipertensión ocular Objective: to compare two models of experimental glaucoma by induction of ocular hypertension in rabbits.Materials and methods: Sixteen New Zealand female rabbits, 2-3 kg were used. Model A (n=6): cauterization of episcleral and perilimbar veins of the right eye (RE) with surgical electrocautery. Model B (n=10): Injection of ?-chymotrypsin in posterior chamber of RE. Intraocular pressure (IOP) was measured before and after the induction of ocular hypertension (OHT), once a week at the same time of day for 40 days, with a manual tonometer. The animals were euthanized by CO2 inhalation. In both models the control was the IOP of the left eye (LE). The mean and standard error (SE) values of IOP, expressed in mmHg, were compared statistically by applying Student's t-test with a significance level of p<0.05. Results: The IOP in LE (control) of model A: was 12.9±1.05 and in model B: 12.9±1.09. There were no significant differences between the models. Model A: The IOP increase in RE was 14.7% (14.8±1.4) with respect to LE. A significant increase in IOP was observed within the first 24 hours: 23.5±1.9 (p<0.05) compared to the control eye. There were no significant differences with subsequent controls. Model B: The increase in IOP in RE was 129.1% (29.6±3.4) with respect to LE. In all cases an increase was observed from Day 1 (p<0.05). The IOP peak in RE was evidenced on Day 25: 35±3.4 (p<0.05). The increase in IOP induced by model B was significantly higher (p<0.01) than in model A. There was loss of ganglion cells of the retina in both models, but the following anatomo-pathological changes were observed only in model B: buphthalmos, subluxation of the lens and increased excavation of the papilla. Conclusion This study indicates that model B is the most appropriate method to induce a rapid, controlled increase of IOP in rabbits and, more importantly, that this increase may be sustained over extended periods of time. This model could be useful for evaluating the efficacy of new ocular drug delivery systems and for further studies of the physiopathology of glaucoma. Objetivo: comparar dos modelos de glaucoma experimental por inducción de hipertensión ocular en conejos y describir cambios anatomo-patológicos. Materiales y métodos: Se utilizaron 16 conejos New Zealand, hembras, de 2-3 kg. Modelo A (n=6): cauterización de venas epiesclerales y perilimbares en ojo derecho (OD) con cauterio eléctrico quirúrgico. Modelo B (n=10): inyección intracamerular en OD de ?-quimotripsina. Se midió la presión intraocular (PIO) antes y después de la inducción de la hipertensión ocular (HTO), una vez por semana, a la misma hora del día, durante 40 días, con tonómetro manual. Los animales fueron sacrificados por inhalación de CO2. En ambos modelos la PIO del ojo izquierdo (OI).fue tomado como valor control. La media y error estándar (EE) de los valores de la PIO, expresada en mmHg, fueron evaluadas y comparadas estadísticamente aplicando Test T de Student considerando un nivel de significación de p < 0.05. Resultados: La PIO en OI (control) del modelo A: fue 12,9±1,05 y en el modelo B: 12,9±1,09. No se observaron diferencias significativas entre ambas. Modelo A: el aumento de la PIO en OD fue 14,7% (14,8±1,4) con respecto a OI. Se observó un incremento significativo de la PIO dentro de las primeras 24 hs: 23,5±1,9 (p<0,05) comparado con el valor del ojo control. No hubo diferencias significativas con los controles posteriores. Modelo B: el aumento de la PIO en OD fue 129,1% (29,6±3,4) con respecto al OI. En todos los casos se observó un incremento desde el día 1 (p<0,05). El pico de PIO en OD se evidenció el día 25: 35±3,4 (p<0,05). El incremento de la PIO inducida en el modelo B fue significativamente mayor (p<0,01) que en el modelo A. En ambos modelos hubo pérdida de células ganglionares de la retina, pero sólo en el modelo B se observaron los siguientes cambios anatomo-patológicos: buftalmus, subluxación del cristalino y aumento de la excavación de la papila. Conclusión: De acuerdo a este estudio, el modelo B aparece como el método más apropiado a los fines de inducir un incremento rápido y controlado de la IOP en conejos y más importante, este incremento sería capaz de mantenerse alto a lo largo de periodos de tiempos extendidos. Este modelo podría ser de gran utilidad para evaluar la eficacia de nuevos sistemas oculares de liberación de fármacos y realizar futuros estudios de la fisiopatología del glaucoma. Universidad Nacional Cba. Facultad de Ciencias Médicas. Secretaria de Ciencia y Tecnología 2018-02-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf image/jpeg image/jpeg image/jpeg application/msword https://revistas.unc.edu.ar/index.php/med/article/view/16811 Revista de la Facultad de Ciencias Médicas de Córdoba.; Vol. 75 No. 1 (2018); 25-31 Revista de la Facultad de Ciencias Médicas de Córdoba; Vol. 75 Núm. 1 (2018); 25-31 Revista da Faculdade de Ciências Médicas de Córdoba; v. 75 n. 1 (2018); 25-31 1853-0605 0014-6722 10.31053/1853.0605.v75.n1 spa eng https://revistas.unc.edu.ar/index.php/med/article/view/16811/20018 https://revistas.unc.edu.ar/index.php/med/article/view/16811/21314 https://revistas.unc.edu.ar/index.php/med/article/view/16811/26408 https://revistas.unc.edu.ar/index.php/med/article/view/16811/26409 https://revistas.unc.edu.ar/index.php/med/article/view/16811/26410 https://revistas.unc.edu.ar/index.php/med/article/view/16811/26561 Derechos de autor 2018 Universidad Nacional de Córdoba |