DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae).
coryne is a tree is tree-sized plant, with large and infundibuliform flowers (12 -15 cm). It is native to the deserts of northwestern Argentina and Bolivia, and is the only species of the genus Stetsonia.The aim of this study was to characterize this species cytologically. For this, the nuclear DNA...
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Sociedad Argentina de Botánica
2016
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| Acceso en línea: | https://revistas.unc.edu.ar/index.php/BSAB/article/view/14846 |
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I10-R10-article-148462020-03-09T17:58:14Z DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). Contenido de ADN y patrón de genes ribosomales en el género monotípico Stetsonia (Cactaceae). Bauk, Karen Santiñaque, Federico López-Carro, Beatriz Las Peñas, M. L. Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. coryne is a tree is tree-sized plant, with large and infundibuliform flowers (12 -15 cm). It is native to the deserts of northwestern Argentina and Bolivia, and is the only species of the genus Stetsonia.The aim of this study was to characterize this species cytologically. For this, the nuclear DNA content, chromosome number, karyotype, banding patterns and location of ribosomal genes of the species wasdetermined. The DNA content analysis revealed a mixture of nuclei with three peaks, 2C, 4C and 8C, indicating a process of endopoliploidy on the species, being this the first report for the tribe Browningieae.It presented chromosome number of 2n = 22, with a symmetrical karyotype of 22 m, with the first pair with satellite. With fluorescent chromosome banding technique, two types of bands were observed: CMA+/ DAPI- associated centromere and NOR associated. With FISH technique, we observed that 18-5.8-26S loci were consistent with CMA+/DAPI-/NORs blocks. The signal for the 5S gene is located in a centromeric region (pair 10). Stetsonia coryne es una planta de porte arborescente y con flores grandes (12 a 15 cm de largo) e infundibuliformes. Es originaria de los desiertos del noroeste de Argentina y Bolivia, y es la única especie del género Stetsonia. El objetivo de este trabajo fue caracterizar citológicamente a esta especie. Para ello se determinó el contenido de ADN nuclear, el número cromosómico, cariotipo, patrones de bandeo, y localización de genes ribosómicos de la especie. El análisis de contenido de ADN reveló una mezcla de núcleos con tres picos de 2C, 4C y 8C, lo que sugiere un proceso de endopoliploidía en la especie, siendo esto el primer reporte para la tribu Browningieae. Presentó número cromosómico somático de 2n=22. Con la técnica de bandeo cromosómico fluorescente se identificaron dos tipos de bandas. Por último, se aplicó la técnica de FISH, se observó una co-localización del locus 18-5,8-26S con la banda CMA+/DAPI-/NORs, la señal del 5S fue localizada en el par 10 en la región centromérica. Estos resultados son los primeros para la tribu. Sociedad Argentina de Botánica 2016-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original application/pdf https://revistas.unc.edu.ar/index.php/BSAB/article/view/14846 Boletín de la Sociedad Argentina de Botánica (Journal of the Argentine Botanical Society; Vol. 51 No. 2 (2016): June; 323-330 Boletín de la Sociedad Argentina de Botánica; Vol. 51 Núm. 2 (2016): Junio; 323-330 Boletín de la Sociedad Argentina de Botánica; v. 51 n. 2 (2016): Junio; 323-330 1851-2372 0373-580X 10.31055/1851.2372.v51.n2 spa https://revistas.unc.edu.ar/index.php/BSAB/article/view/14846/14800 Derechos de autor 2016 Boletín de la Sociedad Argentina de Botánica |
| institution |
Universidad Nacional de Córdoba |
| institution_str |
I-10 |
| repository_str |
R-10 |
| container_title_str |
Revistas de la UNC |
| language |
Español |
| format |
Artículo revista |
| topic |
Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. |
| spellingShingle |
Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. Bauk, Karen Santiñaque, Federico López-Carro, Beatriz Las Peñas, M. L. DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| topic_facet |
Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. Stetsonia coryne contenido de ADN endopoliploidía heterocromatina genes ribosomales. |
| author |
Bauk, Karen Santiñaque, Federico López-Carro, Beatriz Las Peñas, M. L. |
| author_facet |
Bauk, Karen Santiñaque, Federico López-Carro, Beatriz Las Peñas, M. L. |
| author_sort |
Bauk, Karen |
| title |
DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| title_short |
DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| title_full |
DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| title_fullStr |
DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| title_full_unstemmed |
DNA content and pattern of ribosomal genes in the monotypic genus Stetsonia (Cactaceae). |
| title_sort |
dna content and pattern of ribosomal genes in the monotypic genus stetsonia (cactaceae). |
| description |
coryne is a tree is tree-sized plant, with large and infundibuliform flowers (12 -15 cm). It is native to the deserts of northwestern Argentina and Bolivia, and is the only species of the genus Stetsonia.The aim of this study was to characterize this species cytologically. For this, the nuclear DNA content, chromosome number, karyotype, banding patterns and location of ribosomal genes of the species wasdetermined. The DNA content analysis revealed a mixture of nuclei with three peaks, 2C, 4C and 8C, indicating a process of endopoliploidy on the species, being this the first report for the tribe Browningieae.It presented chromosome number of 2n = 22, with a symmetrical karyotype of 22 m, with the first pair with satellite. With fluorescent chromosome banding technique, two types of bands were observed: CMA+/ DAPI- associated centromere and NOR associated. With FISH technique, we observed that 18-5.8-26S loci were consistent with CMA+/DAPI-/NORs blocks. The signal for the 5S gene is located in a centromeric region (pair 10). |
| publisher |
Sociedad Argentina de Botánica |
| publishDate |
2016 |
| url |
https://revistas.unc.edu.ar/index.php/BSAB/article/view/14846 |
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2022-08-20T01:20:37Z |
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