Rate of Protein Glycosylation in Rat Cerebral Cortex
Abstract: Quantitative aspects of the pathway leading to the formation of asparagine‐linked oligosaccharides were investigated in rat cerebral cortex. Steady‐state labeling conditions were achieved with [2‐3H]mannose by developing a micromethod of incubation of cerebral cortex particles in the prese...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1986
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 08858caa a22009377a 4500 | ||
|---|---|---|---|
| 001 | PAPER-994 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203021.0 | ||
| 008 | 190411s1986 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022455684 | |
| 024 | 7 | |2 cas |a Glucose, 50-99-7; Glycoproteins; Leucine, 61-90-5; Mannose, 31103-86-3; Nerve Tissue Proteins; Tritium, 10028-17-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Aplerin, D.M. | |
| 245 | 1 | 0 | |a Rate of Protein Glycosylation in Rat Cerebral Cortex |
| 260 | |c 1986 | ||
| 270 | 1 | 0 | |m Idoyaga‐Vargas, V.P.; Lnstituto de Investigaciones Bioquimicas "Fundacion Campomar", Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Antonio Machado 151, Buenos Aires, 1405, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Alperin, D.M., Idoyaga‐Vargas, V.P., Carminatti, H., Method for the detection of organic compounds on paper chromatograms with high recovery of the sample (1982) J. Chromatogr., 242, pp. 299-304 | ||
| 504 | |a Catteral, W.A., The molecular basis of neuronal excitability (1984) Science, 223, pp. 653-661 | ||
| 504 | |a Goru, F.K., Hooghe‐Peters, E.L., Pipeleers, D.G., Glucose metabolism in murine fetal cortical brain cells: lack of insulin effects (1984) J. Cell. Physiol., 121, pp. 45-50 | ||
| 504 | |a Grant, S.R., Lennarz, W.J., Relationship between oligosaccharide‐lipid synthesis and protein synthesis in mouse LM cells (1983) Eur. J. Biochem., 134, pp. 575-583 | ||
| 504 | |a Hubbard, S.C., Robbins, P.W., Synthesis of N‐linked oligosaccharides of glycoproteins. Assembly of the lipid‐linked precursor oligosaccharide and its relation to protein synthesis in vivo (1980) J. Biol. Chem., 255, pp. 11782-11793 | ||
| 504 | |a Idoyaga‐Vargas, V., Carminatti, H., Glycosylation on endogenous protein(s) of the rough and smooth microsomes by a lipid sugar intermediate (1977) Mol. Cell. Biochem., 16, pp. 171-176 | ||
| 504 | |a Idoyaga‐Vargas, V., Carminatti, H., Postnatal changes in dolichol pathway enzyme activities in cerebral cortex neurons (1982) Biochem. J., 202, pp. 87-95 | ||
| 504 | |a Kornfeld, R., Kornfeld, S., Structure of glycoproteins and their oligosaccharide units (1981) The Biochemistry of Glycoproteins and Proteoglycans, pp. 1-28. , Lennarz W. J., ed, pp., Plenum Press, New York | ||
| 504 | |a Lajtha, A.L., Maker, H., Clarke, D.D., Metabolism and transport of carbohydrates and amino acids (1981) Basic Neurochemistry, pp. 329-353. , Siegel G. J., Albers R. W., Agranoff B. W., Katzman R., eds, pp, Little, Brown, Boston, Massachusetts | ||
| 504 | |a Lucas, J.J., Levin, E., Increase in the lipid intermediate pathway of protein glycosylation during hen oviduct differentiation (1977) J. Biol. Chem., 252, pp. 4330-4336 | ||
| 504 | |a Lund‐Andersen, H., Transport of glucose from blood to brain (1979) Physiol. Rev., 59, pp. 305-352 | ||
| 504 | |a Structure and distribution of glycoproteins and glycosaminoglycans (1979) Complex Carbohydrates of Nervous Tissue, pp. 45-73. , Margolis R. U., Margolis R. K., eds, Plenum Press, New York | ||
| 504 | |a Merlie, J.P., Sebbane, R., Gardner, S., Olson, E., Lindstrom, J., The regulation of acetylcholine receptor expression in mammalian muscle. Cold Spring Harbor Symp (1983) Quant. Biol., 48, pp. 136-146 | ||
| 504 | |a Morgan, M.J., Faik, P., Carbohydrate metabolism in cultured animal cells (1981) Biosci. Rep., 1, pp. 669-686 | ||
| 504 | |a Oda‐Tamai, S., Kato, S., Hara, S., Akamatsu, N., Decreased transfer of oligosaccharide from oligosaccharide lipid to protein acceptors in regenerating liver (1985) J. Biol. Chem., 260, pp. 57-63 | ||
| 504 | |a Pardridge, W.M., Brain metabolism: a perspective from the blood‐brain barrier (1983) Physiol. Rev., 63, pp. 1481-1535 | ||
| 504 | |a Parodi, A.J., Quesada‐Allue, L., Cazzulo, J.J., Pathway of glycosylation in the trypanosomatid Crithidia fasciculata (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 6201-6205 | ||
| 504 | |a Rosenberg, R.N., Regulation of neuronal enzymes in cell culture (1973) Tissue Culture of the Nervous System, pp. 107-134. , Sato G., ed, pp., Plenum Press, New York and London | ||
| 504 | |a Rossignol, W.J., Waechter, C.J., Induction of phosphorylation of dolichol during embryonic development of the sea urchin (1981) J. Biol. Chem., 256, pp. 10538-10542 | ||
| 504 | |a Sellinger, O.Z., Johnson, D.E., Santiago, J., Idoyaga‐Vargas, V., A study of the biochemical differentiation of neurons and glia in the rat cerebral cortex (1973) Progress in Brain Research, Vol. 40: Neurobiological Aspects of Maturation and Aging, pp. 331-347. , Ford D. H., ed, pp., Elsevier, Amsterdam | ||
| 504 | |a Sharon, N., Glycoprotein biosynthesis (1975) Complex Carbohydrates, pp. 118-141. , Addison‐Wesley, Reading, Massachusetts | ||
| 504 | |a Silverman, J.A., Amenta, J.S., Sources of error in estimating radioactivity in protein from cell cultures by liquid scintillation counting (1984) Anal. Biochem., 141, pp. 538-544 | ||
| 504 | |a Staneloni, R.J., Leloir, L.F., The biosynthetic pathway of the asparagine‐linked oligosaccharides of glycoproteins (1982) Crit. Rev. Biochem., 12, pp. 289-326 | ||
| 504 | |a Tower, D.B., Cerebral edema (1976) Basic Neurochemistry, pp. 627-645. , Siegel G. J., Wayne Albers R., Katzman R., Agranoff B. W., eds, pp., Little. Brown, Boston, Massachusetts | ||
| 504 | |a Welply, J.K., Lau, J.T., Lennarz, W.J., Developmental regulation of glycosyltransferases involved in synthesis of N‐linked glycoproteins in sea urchin embryos (1985) Dev. Biol., 107, pp. 252-258 | ||
| 504 | |a Zanetta, J.P., Gombos, G., Affinity chromatography on Con A‐Sepharose of synaptic vesicle membrane glycoproteins (1974) FEBS Lett., 47, pp. 276-278 | ||
| 520 | 3 | |a Abstract: Quantitative aspects of the pathway leading to the formation of asparagine‐linked oligosaccharides were investigated in rat cerebral cortex. Steady‐state labeling conditions were achieved with [2‐3H]mannose by developing a micromethod of incubation of cerebral cortex particles in the presence of physiological concentrations of glucose (1 g/L). The rate of [2‐3H]mannose uptake and incorporation into protein was markedly affected when the concentration of glucose was lowered to 0.05 g/L. It was found that in the presence of glucose (1 g/L), a minor fraction of the utilized [2‐3H]mannose is used in glyco‐protein formation and the remaining labeled sugar enters the other major metabolic pathways, generating tritiated water which is rapidly exchanged with that of the medium. Under these conditions, the intracellular isotopic dilution of [2‐3H]mannose‐labeled precursors was calculated to be about 11.5‐fold. These data allow determination of the rate of the net transfer of mannose into proteins. Comparison of the rate of glycosylation between 5‐ and 30‐day‐old cerebral cortex revealed a striking difference: 2.1 and 0.3 ng of mannose/mg protein/h, respectively. Copyright © 1986, Wiley Blackwell. All rights reserved |l eng | |
| 593 | |a Instituto de Investigaciones Bioquímicas "Fundación Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CEREBRAL CORTEX |
| 690 | 1 | 0 | |a DEVELOPMENT |
| 690 | 1 | 0 | |a GLYCOSYLATION RATE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a BRAIN CORTEX |
| 690 | 1 | 0 | |a CENTRAL NERVOUS SYSTEM |
| 690 | 1 | 0 | |a MANNOSE H 3 |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN GLYCOSYLATION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a AGING |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CEREBRAL CORTEX |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a DIFFUSION |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLYCOPROTEINS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LEUCINE |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a NERVE TISSUE PROTEINS |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, INBRED STRAINS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRITIUM |
| 700 | 1 | |a Idoyaga-Vargas, V.P. | |
| 700 | 1 | |a Carminatti, H. | |
| 773 | 0 | |d 1986 |g v. 47 |h pp. 355-362 |k n. 2 |p J. Neurochem. |x 00223042 |w (AR-BaUEN)CENRE-737 |t Journal of Neurochemistry | |
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| 856 | 4 | 0 | |u https://doi.org/10.1111/j.1471-4159.1986.tb04509.x |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00223042_v47_n2_p355_Aplerin |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223042_v47_n2_p355_Aplerin |y Registro en la Biblioteca Digital |
| 961 | |a paper_00223042_v47_n2_p355_Aplerin |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 61947 | ||