Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this invitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin m...
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2012
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 11563caa a22012857a 4500 | ||
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| 001 | PAPER-9889 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203952.0 | ||
| 008 | 140217s2012 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84855470663 | |
| 024 | 7 | |2 pubmed |a 22133569 | |
| 024 | 7 | |2 cas |a metalloproteinase, 81669-70-7; phospholipase A2, 9001-84-7; snake venom, 55230-69-8; Antibodies, Monoclonal; Crotalid Venoms; Metalloproteases, 3.4.-; Phospholipases A2, 3.1.1.4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a TOXIA | ||
| 100 | 1 | |a Bustillo, S. | |
| 245 | 1 | 0 | |a Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells |
| 260 | |c 2012 | ||
| 270 | 1 | 0 | |m Leiva, L.C.; Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste (UNNE), Av. Libertad 5470, CP 3400, Corrientes, Argentina; email: lauraleiva2004@yahoo.com.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Angulo, Y., Lomonte, B., Differential susceptibility of C2C12 myoblasts and myotubes to group II phospholipase A2 myotoxins from crotalid snake venoms (2005) Cell Biochem. Funct., 23, pp. 307-313 | ||
| 504 | |a Bonfim, V.L., de Carvalho, D.D., Ponce-Soto, L.A., Kassab, B.H., Marangoni, S., Toxicity of phospholipases A2 D49 (6-1 and 6-2) and K49 (Bj-VII) from Bothrops jararacussu venom (2009) Cell Biol. Toxicol., 25, pp. 523-532 | ||
| 504 | |a Borkow, G., Gutierrez, J.M., Ovadia, M., Invitro activity of BaH1, the main hemorrhagic toxin of Bothrops asper snake venom on bovine endothelial cells (1995) Toxicon, 33, pp. 1387-1391 | ||
| 504 | |a Brenes, O., Munoz, E., Roldan-Rodriguez, R., Diaz, C., Cell death induced by Bothrops asper snake venom metalloproteinase on endothelial and other cell lines (2010) Exp. Mol. Pathol., 88, pp. 424-432 | ||
| 504 | |a Bustillo, S., Lucero, H., Leiva, L.C., Acosta, O., Kier Joffé, E.B., Gorodner, J.O., Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina (2009) J. Venom Anim. Toxins Incl. Trop. Dis., 15, pp. 28-42 | ||
| 504 | |a Cintra-Francischinelli, M., Pizzo, P., Angulo, Y., Gutierrez, J.M., Montecucco, C., Lomonte, B., The C-terminal region of a Lys49 myotoxin mediates Ca(2+) influx in C2C12 myotubes (2009) Toxicon | ||
| 504 | |a Chavez-Olortegui, C., Penaforte, C.L., Silva, R.R., Ferreira, A.P., Rezende, N.A., Amaral, C.F., Diniz, C.R., An enzyme-linked immunosorbent assay (ELISA) that discriminates between the venoms of Brazilian Bothrops species and Crotalus durissus (1997) Toxicon, 35, pp. 253-260 | ||
| 504 | |a Diaz, C., Valverde, L., Brenes, O., Rucavado, A., Gutierrez, J.M., Characterization of events associated with apoptosis/anoikis induced by snake venom metalloproteinase BaP1 on human endothelial cells (2005) J. Cell Biochem., 94, pp. 520-528 | ||
| 504 | |a Fernandez, J., Gutierrez, J.M., Angulo, Y., Sanz, L., Juarez, P., Calvete, J.J., Lomonte, B., Isolation of an acidic phospholipase A(2) from the venom of the snake Bothrops asper of Costa Rica: biochemical and toxicological characterization (2010) Biochimie, 92, pp. 273-283 | ||
| 504 | |a Garcia Denegri, M.E., Acosta, O.C., Huancahuire-Vega, S., Martins-de-Souza, D., Marangoni, S., Marunak, S.L., Teibler, G.P., Ponce-Soto, L.A., Isolation and functional characterization of a new acidic PLA(2) Ba SpII RP4 of the Bothrops alternatus snake venom from Argentina (2010) Toxicon, 56, pp. 64-74 | ||
| 504 | |a Gay, C.C., Leiva, L.C., Marunak, S., Teibler, P., Acosta de Perez, O., Proteolytic, edematogenic and myotoxic activities of a hemorrhagic metalloproteinase isolated from Bothrops alternatus venom (2005) Toxicon, 46, pp. 546-554 | ||
| 504 | |a Gutiérrez, J.M., Avila, C., Rojas, E., Cerdas, L., An alternative invitro method for testing the potency of the polyvalent antivenom produced in Costa Rica (1988) Toxicon, 26, pp. 411-413 | ||
| 504 | |a Gutiérrez, J.M., Ownby, C.L., Skeletal muscle degeneration induced by venom phospholipases A2: insights into the mechanisms of local and systemic myotoxicity (2003) Toxicon, 42, pp. 915-931 | ||
| 504 | |a Gutiérrez, J.M., Ponce-Soto, L.A., Marangoni, S., Lomonte, B., Systemic and local myotoxicity induced by snake venom group II phospholipases A2: comparison between crotoxin, crotoxin B and a Lys49 PLA2 homologue (2008) Toxicon, 51, pp. 80-92 | ||
| 504 | |a Gutiérrez, J.M., Romero, M., Nunez, J., Chaves, F., Borkow, G., Ovadia, M., Skeletal muscle necrosis and regeneration after injection of BaH1, a hemorrhagic metalloproteinase isolated from the venom of the snake Bothrops asper (Terciopelo) (1995) Exp. Mol. Pathol., 62, pp. 28-41 | ||
| 504 | |a Gutiérrez, J.M., Rucavado, A., Escalante, T., Díaz, C., Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanism involved in microvessel damage (2005) Toxicon, 45, pp. 997-1011 | ||
| 504 | |a Kondo, H., Kondo, S., Ikezawa, H., Murata, R., Studies on the quantitative method for determination of hemorrhagic activity of Habu snake venom (1960) Jpn. J. Med. Sci. Biol., 13, pp. 43-52 | ||
| 504 | |a Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 | ||
| 504 | |a Lomonte, B., Angulo, Y., Rufini, S., Cho, W., Giglio, J.R., Ohno, M., Daniele, J.J., Gutierrez, J.M., Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells invitro (1999) Toxicon, 37, pp. 145-158 | ||
| 504 | |a Lomonte, B., Tarkowski, A., Hanson, L.A., Broad cytolytic specificity of myotoxin II, a lysine-49 phospholipase A2 of Bothrops asper snake venom (1994) Toxicon, 32, pp. 1359-1369 | ||
| 504 | |a Montecucco, C., Gutierrez, J.M., Lomonte, B., Cellular pathology induced by snake venom phospholipase A2 myotoxins and neurotoxins: common aspects of their mechanisms of action (2008) Cell Mol. Life Sci., 65, pp. 2897-2912 | ||
| 504 | |a Otero, R., Gutierrez, J., Beatriz Mesa, M., Duque, E., Rodriguez, O., Luis Arango, J., Gomez, F., Diaz, A., Complications of Bothrops, Porthidium, and Bothriechis snakebites in Colombia. A clinical and epidemiological study of 39 cases attended in a university hospital (2002) Toxicon, 40, pp. 1107-1114 | ||
| 504 | |a Ouchterlony, O., Antigen-antibody reactions in gels (1949) Acta Path. Microbiol. Scand., 26, pp. 507-515 | ||
| 504 | |a Rodrigues, V.M., Soares, A.M., Andriao-Escarso, S.H., Franceschi, A.M., Rucavado, A., Gutierrez, J.M., Giglio, J.R., Pathological alterations induced by neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom (2001) Biochimie, 83, pp. 471-479 | ||
| 504 | |a Soares, A.M., Guerra-Sa, R., Borja-Oliveira, C.R., Rodrigues, V.M., Rodrigues-Simioni, L., Rodrigues, V., Fontes, M.R., Giglio, J.R., Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A(2) homologue from Bothrops neuwiedi pauloensis venom (2000) Arch. Biochem. Biophys., 378, pp. 201-209 | ||
| 504 | |a Tanjoni, I., Weinlich, R., Della-Casa, M.S., Clissa, P.B., Saldanha-Gama, R.F., de Freitas, M.S., Barja-Fidalgo, C., Moura-da-Silva, A.M., Jararhagin, a snake venom metalloproteinase, induces a specialized form of apoptosis (anoikis) selective to endothelial cells (2005) Apoptosis, 10, pp. 851-861 | ||
| 504 | |a Trummal, K., Tonismagi, K., Siigur, E., Aaspollu, A., Lopp, A., Sillat, T., Saat, R., Siigur, J., A novel metalloprotease from Vipera lebetina venom induces human endothelial cell apoptosis (2005) Toxicon, 46, pp. 46-61 | ||
| 504 | |a Yaffe, D., Saxel, O., Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle (1977) Nature, 270, pp. 725-727 | ||
| 504 | |a Yamamoto, Y., Nakajima, M., Yamazaki, H., Yokoi, T., Cytotoxicity and apoptosis produced by troglitazone in human hepatoma cells (2001) Life Sci., 70, pp. 471-482 | ||
| 520 | 3 | |a Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this invitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34μg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism. © 2011 Elsevier Ltd. |l eng | |
| 593 | |a Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste (UNNE), Av. Libertad 5470, CP 3400, Corrientes, Argentina | ||
| 593 | |a Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina | ||
| 593 | |a Area Investigación, Instituto de Oncología Angel H. Roffo, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Bioquímica, Instituto de Biología, Universidade Estadual de Campinas, Campinas, SP, Brazil | ||
| 690 | 1 | 0 | |a BALTERGIN |
| 690 | 1 | 0 | |a BOTHROPS ALTERNATUS |
| 690 | 1 | 0 | |a C2C12 |
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a METALLOPROTEINASE |
| 690 | 1 | 0 | |a PHOSPHOLIPASE A2 |
| 690 | 1 | 0 | |a SYNERGISM |
| 690 | 1 | 0 | |a ACIDIC PHOSPHOLIPASE A2 |
| 690 | 1 | 0 | |a BALTERGIN |
| 690 | 1 | 0 | |a METALLOPROTEINASE |
| 690 | 1 | 0 | |a PHOSPHOLIPASE A2 |
| 690 | 1 | 0 | |a SNAKE VENOM |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANOIKIS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BOTHROPS ALTERNATUS |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a ENZYME ISOLATION |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a MYOBLAST |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a POISONOUS SNAKE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIBODIES, MONOCLONAL |
| 690 | 1 | 0 | |a BOTHROPS |
| 690 | 1 | 0 | |a CELL LINE |
| 690 | 1 | 0 | |a CROTALID VENOMS |
| 690 | 1 | 0 | |a DRUG SYNERGISM |
| 690 | 1 | 0 | |a METALLOPROTEASES |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MICE, INBRED C3H |
| 690 | 1 | 0 | |a MUSCLE, SKELETAL |
| 690 | 1 | 0 | |a MUSCULAR DISEASES |
| 690 | 1 | 0 | |a PHOSPHOLIPASES A2 |
| 690 | 1 | 0 | |a RABBITS |
| 690 | 1 | 0 | |a BOTHROPS |
| 690 | 1 | 0 | |a BOTHROPS ALTERNATUS |
| 700 | 1 | |a Gay, C.C. | |
| 700 | 1 | |a García Denegri, M.E. | |
| 700 | 1 | |a Ponce-Soto, L.A. | |
| 700 | 1 | |a Bal de Kier Joffé, E. | |
| 700 | 1 | |a Acosta, O. | |
| 700 | 1 | |a Leiva, L.C. | |
| 773 | 0 | |d 2012 |g v. 59 |h pp. 338-343 |k n. 2 |p Toxicon |x 00410101 |w (AR-BaUEN)CENRE-7050 |t Toxicon | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/j.toxicon.2011.11.007 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00410101_v59_n2_p338_Bustillo |y Handle |
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