A new world monkey microsatellite (AP74) higly conserved in primates

Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in bloo...

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Autor principal: Oklander, L.I
Otros Autores: Steinberg, E.R, Mudry, M.D
Formato: Capítulo de libro
Lenguaje:Inglés
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Publicado: 2012
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Acceso en línea:Registro en Scopus
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100 1 |a Oklander, L.I. 
245 1 2 |a A new world monkey microsatellite (AP74) higly conserved in primates 
246 3 1 |a AP74, un microsatélite de monos del nuevo mundo altamente conservado en primates 
260 |c 2012 
270 1 0 |m Oklander, L. I.; IBS (Instituto de Biología Subtropical), Facultad de Ciencias Forestales, Universidad Nacional de Misiones, Andresito 21, Puerto Iguazú, Misiones, Argentina; email: lulaok@gmail.com 
506 |2 openaire  |e Política editorial 
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504 |a Oklander, L.I., Zunino, G.E., di Fiore, A., Corach, D., Isolation, characterization and evaluation of 11 autosomal STRs suitable for population studies in black and gold howler monkeys Alouatta caraya (2006) Mol Ecol Notes, 7, pp. 117-120 
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520 3 |a Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.  |l eng 
593 |a IBS (Instituto de Biología Subtropical), Facultad de Ciencias Forestales, Universidad Nacional de Misiones, Puerto Iguazú, Misiones, Argentina 
593 |a Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina 
593 |a Grupo de Investigación en Biología Evolutiva, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 
593 |a CONICET, Argentina 
690 1 0 |a GENETIC VARIABILITY 
690 1 0 |a GENOMIC CONSERVATION 
690 1 0 |a MICROSATELLITE MARKERS 
690 1 0 |a NON-INVASIVE SAMPLING 
690 1 0 |a PCR AMPLIFICATION 
690 1 0 |a ALOUATTA 
690 1 0 |a ALOUATTA CARAYA 
690 1 0 |a ATELES BELZEBUTH 
690 1 0 |a ATELES BELZEBUTH CHAMEK 
690 1 0 |a ATELES SP. 
690 1 0 |a HAPLORHINI 
690 1 0 |a HOMO SAPIENS 
690 1 0 |a LEMUR 
690 1 0 |a LEMUR CATTA 
690 1 0 |a LEMURIDAE 
690 1 0 |a PAN TROGLODYTES 
690 1 0 |a PAPIO 
690 1 0 |a PAPIO SP. 
690 1 0 |a PLATYRRHINI 
650 1 7 |2 spines  |a PRIMATES 
650 1 7 |2 spines  |a PRIMATES 
700 1 |a Steinberg, E.R. 
700 1 |a Mudry, M.D. 
773 0 |d 2012  |g v. 17  |h pp. 93-101  |k n. 1  |p Acta Biol. Colomb.  |x 0120548X  |w (AR-BaUEN)CENRE-532  |t Acta Biologica Colombiana 
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