Trypanosoma cruzi adenylate cyclase activity. Purification and characterization
Adenylate cyclase activity associated with Trypanosoma cruzi sedimentable fractions was solubilized by treatment with the non-ionic detergent Lubrol PX and 0.5 M-(NH4)2SO4. The following hydrodynamic and molecular parameters were established for a partially purified enzyme-detergent complex: sedimen...
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| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1986
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03093caa a22005417a 4500 | ||
|---|---|---|---|
| 001 | PAPER-950 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203017.0 | ||
| 008 | 190411s1986 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022520965 | |
| 024 | 7 | |2 cas |a adenylate cyclase, 9012-42-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BIJOA | ||
| 100 | 1 | |a Torruella, M. | |
| 245 | 1 | 0 | |a Trypanosoma cruzi adenylate cyclase activity. Purification and characterization |
| 260 | |c 1986 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Adenylate cyclase activity associated with Trypanosoma cruzi sedimentable fractions was solubilized by treatment with the non-ionic detergent Lubrol PX and 0.5 M-(NH4)2SO4. The following hydrodynamic and molecular parameters were established for a partially purified enzyme-detergent complex: sedimentation coefficient 6.2 S; Stokes radius 5.65 nm; partial specific volume 0.83 ml/g; M(r) 244000; frictional ratio 1.33. A M(r) of about 124000 was calculated for the detergent-free protein from these parameters. The pI of this enzyme activity was 6.2. A monoclonal antibody to T. cruzi adenylate cyclase was obtained, which inhibited cyclase activities from several lower eukaryotic organisms. The T. cruzi adenylate cyclase was further purified by using this antibody in immunoaffinity chromatographic columns. Fractions obtained after this chromatography showed, on SDS/polyacrylamide-gel electrophoresis, a main polypeptide band with an apparent M(r) of about 56000, which specifically reacted with the monoclonal antibody. |l eng | |
| 593 | |a Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a ENZYME |
| 690 | 1 | 0 | |a MONOCLONAL ANTIBODY |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ELECTROPHORESIS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTOZOON |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a EUKARYOTA |
| 690 | 1 | 0 | |a PROTOZOA |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Flawia, M.M. | |
| 700 | 1 | |a Eisenschlos, C. | |
| 700 | 1 | |a Molina y Vedia, L. | |
| 700 | 1 | |a Rubinstein, C.P. | |
| 700 | 1 | |a Torres, H.N. | |
| 773 | 0 | |d 1986 |g v. 234 |h pp. 145-150 |k n. 1 |p BIOCHEM. J. |x 02646021 |w (AR-BaUEN)CENRE-205 |t Biochemical Journal | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022520965&doi=10.1042%2fbj2340145&partnerID=40&md5=c1928bebac47d68354a45157d467673a |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1042/bj2340145 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_02646021_v234_n1_p145_Torruella |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v234_n1_p145_Torruella |y Registro en la Biblioteca Digital |
| 961 | |a paper_02646021_v234_n1_p145_Torruella |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 61903 | ||