Heme synthetase in Trypanosoma cruzi
1. 1. Heme-synthetase (Heme-S) has been studied in the epimastigote form of T. cruzi (Tulahuen and Y strains). 2. 2. The enzyme is confined to the "mitochondrial" fraction (sedimented at 30,000g). 3. 3. Activity was dependent on protein and time of cell storage. 4. 4. Enzymic proto- and me...
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1986
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 08485caa a22008417a 4500 | ||
|---|---|---|---|
| 001 | PAPER-943 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203017.0 | ||
| 008 | 190411s1986 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0022961774 | |
| 024 | 7 | |2 cas |a ferrochelatase, 9012-93-5; iron, 14093-02-8, 53858-86-9, 7439-89-6; lyase, 9055-04-3; Cations, Divalent; Ferrochelatase, EC 4.99.1.1; Iron, 7439-89-6; Lyases, EC 4. | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Salzman, T.A. | |
| 245 | 1 | 0 | |a Heme synthetase in Trypanosoma cruzi |
| 260 | |c 1986 | ||
| 270 | 1 | 0 | |m del C. Batlle, A.M.; Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP, (CONICET-FCEN, UBA), Ciudad Universitaria, Pab. II, 1428 Nuñez Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Chang, Sassa, Heme biosynthesis in bacterium-protozoon symbiosis: enzymic defects in host hemoflagellates and complemental role of their intracellular symbiotes (1975) Proc. natn. Acad. Sci., U.S.A., 72, pp. 2979-2983 | ||
| 504 | |a Docampo, de, Stoppani, Tricarboxylic acid cycle operation at the kinetoplastmitochondrion complex of Trypanosoma cruzi (1978) Biochim. Biophys. Acta, 502, pp. 466-476 | ||
| 504 | |a Gaugham, Krassner, Hemin deprivation in culture stages of hemoflagellates Leishmania tarentolae (1971) Comp. Biochem. Physiol., 39 B, pp. 5-18 | ||
| 504 | |a Hutner, Bacchi, Baker, Nutrition of the kinetoplastida (1979) Biology of the kinetoplastida, pp. 654-691. , W.H.R. Lumsden, D.A. Evans, Academic Press, New York, N°2 | ||
| 504 | |a Jensen, Thofern, Clhoramin (ferroporphyrin chloride) als bakterienurichsstoff (1953) Naturforshung, 86, pp. 599-607 | ||
| 504 | |a Johnson, Jones, Enzymic formation of haems and other metalloporphyrins (1964) Biochim. Biophys. Acta, 93, pp. 171-173 | ||
| 504 | |a Lochhead, Goldberg, The enzymic formation of haem by human and rat tissues (1961) Biochem. J., 78, pp. 146-150 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Lwoff, The nutrition of parasitic flagellates (Trypanosomidae, Trichomonadinae) (1951) Biochemistry and Physiology of Protazoa, pp. 131-182. , A. Lwoff, Academic Press, New York | ||
| 504 | |a Porra, Jones, Studies on ferrochelatase I. Assay and properties of ferrochelatase from a pig liver mitochondrial extract (1963) Biochem. J., 87, pp. 181-185 | ||
| 504 | |a Porra, Jones, An investigation of the role of ferrochelatase in the biosynthesis of various haem groups (1963) Biochem. J., 87, pp. 186-192 | ||
| 504 | |a Salzman, Batlle, Angluster, De Souza, Heme synthesis in Crithidia deanei: influence of the endosymbiote (1985) Int. J. Biochem., 17, pp. 1343-1347 | ||
| 504 | |a Salzman, Batlle, De Souza, Heme synthesis in Trypanosoma cruzi: influence of the strain and culture medium (1986) Comp. Biochem. Physiol., 86 B, pp. 57-61 | ||
| 504 | |a Salzman, Stella, Wider de Xifra, Batlle, Docampo, Stoppani, Porphyrin biosynthesis in parasitic hemoflagellates functional and defective enzymes in Trypanosoma cruzi (1982) Comp. Biochem. Physiol., 72 B, pp. 663-667 | ||
| 504 | |a Simpson, Poulson, Effects of lipids on the activity of ferrochelatase (1977) Biochim. biophys. Acta, 482 (2), pp. 461-469 | ||
| 504 | |a Taketani, Tokunaga, Rat liver ferrochelatase (1981) Purification, properties and stimulation by fatty acids, 256, pp. 12748-12753. , J. biol. Chem | ||
| 504 | |a Tokunaga, Sano, Comparative studies of non-enzymic and enzymic protoheme formation (1972) Biochim. biophys. Acta, 264, pp. 263-271 | ||
| 504 | |a Ulvik, Romslo, Studies on the utilization of ferritin iron in the ferrochelatase reaction of isolated rat liver mitochondria (1978) Biochim. biophys. Acta, 541, pp. 251-262 | ||
| 504 | |a Warren, Metabolism of Schizotrypanum cruzi Chagas I. Effect of culture age and substrate concentration on respiratory rate (1960) J. Parasit., 46, pp. 529-539 | ||
| 520 | 3 | |a 1. 1. Heme-synthetase (Heme-S) has been studied in the epimastigote form of T. cruzi (Tulahuen and Y strains). 2. 2. The enzyme is confined to the "mitochondrial" fraction (sedimented at 30,000g). 3. 3. Activity was dependent on protein and time of cell storage. 4. 4. Enzymic proto- and meso-heme formation was inhibited up to 40 and 72% respectively by Triton X-100. 5. 5. The optimum pH was 7.2 for protoheme and 7.5 for mesoheme formation. 6. 6. Heme-S reached its maximum when the concentration values were 37; 35 and 32 μM for proto-, meso-, and deuteroporphyrin, respectively. The activity is several times higher when mesoporphyrin is used as substrate. 7. 7. At a final cone of 100 μM Fe2+ and Zn2+ ions enhanced activity 200-400%. Cu2+ and Co2+ had no effect, while Mn2+ and Mg2+ were highly inhibitory. A combination of Fe2+ and Zn2+ at varying concentrations still showed great activation. However, at a fixed level of Fe2+, Cu2+ was changed into a strong inhibitor. 8. 8. We propose that, if it can be demonstrated that T. cruzi cannot multiply when protoheme is replaced by mesoheme, administration of mesoporphyrin would then greatly affect replication of T. cruzi. Furthermore, the addition of certain metals, such as Cu2+ to T. cruzi cultures might specifically inhibit the parasite enzyme opening the possibility of selectively destroying the hemoflagellate without affecting the host. © 1986. |l eng | |
| 536 | |a Detalles de la financiación: Secretaria de Ciencia y Tecnica, Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgements--This work was supported by grants from the Consejo Nacional de Investigaciones Cientificas y T6nicas (CONICET), B. Aires, Argentine, The SECYT, Banco de la Naci6n Argentina, Min. de Salud Pfiblica. A. M. del C. Batlle holds the post of Principal Scientific Researcher in the CONICET. This work forms part of the Thesis of T. A. Salzman to be submitted to the University of Buenos Aires for her Ph.D. degree. We are very grateful to Dr Roberto Docampo and Prof. Andr6s O. M. Stoppani from the CIBIERG (CONICET and FCM-UBA) for their most valuable support throughout this work and providing us with the Tulahuen strain of T. cruzi; also to Dr Ana SteUa and Eva Wider for their help in the performance of some preliminary experiments. We extend our thanks to Dr Wanderley De Souza (Instituto de Biofisica-UFRJ, R. J., Brasil) for generously giving us the Y strain of 7". cruzi. The technical assistance of Mrs B. Riccillo is also acknowledged. Finally we also wish to express our gratitude to Arquitect Hugo E. Salzman for the excellent drawings and to Miss Claudia Tedesco for help with the final typing. | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP, (CONICET-FCEN, UBA), Ciudad Universitaria, Pab. II, 1428 Nuñez Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a DIVALENT CATION |
| 690 | 1 | 0 | |a FERROCHELATASE |
| 690 | 1 | 0 | |a IRON |
| 690 | 1 | 0 | |a LYASE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL FRACTIONATION |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MITOCHONDRION |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CATIONS, DIVALENT |
| 690 | 1 | 0 | |a FERROCHELATASE |
| 690 | 1 | 0 | |a IRON |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a LYASES |
| 690 | 1 | 0 | |a MITOCHONDRIA |
| 690 | 1 | 0 | |a SUBCELLULAR FRACTIONS |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a del C. Batlle, A.M. | |
| 773 | 0 | |d 1986 |g v. 85 |h pp. 537-541 |k n. 3 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0305-0491(86)90043-X |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03050491_v85_n3_p537_Salzman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v85_n3_p537_Salzman |y Registro en la Biblioteca Digital |
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