Porphyrin biosynthesis in Euglena gracilis-V. Soluble and particulate PBG-ASE
1. 1. Porphobilinogenase (PBG-ase) from soluble and particulate fractions of Euglena gracilis was isolated and purified. 2. 2. A highly efficient method for the dissociation of the membrane-bound enzyme using the chaotropic agent SCN is described. 3. 3. The chromatographic behaviour of both soluble...
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1986
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| LEADER | 12776caa a22009737a 4500 | ||
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| 001 | PAPER-940 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203017.0 | ||
| 008 | 190411s1986 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-46149136677 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Rossetti, M.V. | |
| 245 | 1 | 0 | |a Porphyrin biosynthesis in Euglena gracilis-V. Soluble and particulate PBG-ASE |
| 260 | |c 1986 | ||
| 270 | 1 | 0 | |m Rossetti, M.V.; Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET-FCEN, UBA), Ciudad Universitaria, Pabellon II, 4 to, Piso, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Battersby, Fookes, Matcham, McDonald, Biosynthesis of the pigments of life: formation of the macrocycle (Review) (1980) Nature, 285, pp. 17-21 | ||
| 504 | |a Battersby, Fookes, Gustafson-Potter, McDonald, Matcham, Biosynthesis of porphyrins and related macrocycles. Part 171,2 (1982) Chemical and enzymic transformation of isomeric aminomethylbilanes into uroporphyrinogens: proof that unrearranged bilane is the preferred enzymic substrate and detection of a transient intermediate, 1, pp. 2413-2426. , J. Chem. Soc. Perkin Trans | ||
| 504 | |a Battersby, Fookes, Gustafson-Potter, McDonald, Matcham, Biosynthesis of porphyrins and related macrocycles. Part 181,2 (1982) Proof by spectroscopy and synthesis that unrearranged hydroxymethylbilane is the product from deaminase and the substrate for cosynthetase in the biosynthesis of uroporphyrinogen III, 1, pp. 2427-2444. , J. Chem. Soc. Perkin Trans | ||
| 504 | |a Battersby, Fookes, Matcham, McDonald, Hollenstein, Biosynthesis of porphyrins and related macrocycles. Part 20 (1983) Purification of deaminase and studies on its mode of action, 1, pp. 3031-3040. , J. Chem. Soc. Perkin Trans | ||
| 504 | |a Battersby, Fookes, Matcham, Pandey, Biosynthesis of porphyrins and related macrocycles. Part 21 (1983) The interaction of deaminase and its product (hydroxymethylbilane) and the relationship between deaminase and cosynthetase, 1, pp. 3041-3047. , J. Chem. Soc. Perkin Trans | ||
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| 504 | |a Carrell, Kahn, Synthesis of porphyrins by chloroplasts of Euglena (1964) Arch. biochem. Biophys., 108, pp. 1-6 | ||
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| 504 | |a Falk, Dresel, Pseudouroporphyrin and enzymic and non-enzymic porphyrin formation in vitro (1960) Biochim. biophys. Acta, 39, pp. 458-462 | ||
| 504 | |a Fumagalli, Rossetti, Juknat de Geralnik, Kotler, Batlle, Estudios sobre la PBG-asa de hígado de cerdo (1982) Anal. Asoc. Quim. Arg., 70, pp. 375-382 | ||
| 504 | |a Hankins, Mills, Anthramilate synthase-amido-transferase (combined) (1976) A novel form of anthranilate synthase from Euglena gracilis, 251, pp. 7774-7778. , J. biol. Chem | ||
| 504 | |a Hankins, Mills, A dimer of a single polypeptide chain catalyzes the terminal four reactions of the l-tryptophan pathway in Euglena gracilis (1977) J. biol. Chem., 252, pp. 235-239 | ||
| 504 | |a Hatefi, Hanstein, Solubilization of particulate proteins and nonelectrolytes by chaotropic agents (1969) Proc. Natn. Acad. Sci. U.S.A., 62, pp. 1129-1136 | ||
| 504 | |a Jordan, Berry, Preuroporphyrinogen, a universal intermediate in the biosynthesis of uroporphyrinogen III (1980) FEBS Letters, 12, pp. 86-88 | ||
| 504 | |a Jordan, Berry, Mechanism of action of porphobilinogen deaminase (1981) Biochem. J., 195, pp. 177-181 | ||
| 504 | |a Jordan, Seehra, The biosynthesis of uroporphyrinogen III: order of assembly of the four porphobilinogen molecules in the formation of the tetrapyrrole ring (1979) FEBS Letters, 104, pp. 364-366 | ||
| 504 | |a Jordan, Burton, Nordlov, Scheneider, Pryde, Scott, Pre-uroporphyrinogen a substrate for uroporphyrinogen III cosynthetase (1979) Journal of the Chemical Society, Chemical Communications, pp. 204-205 | ||
| 504 | |a Juknat de Geralnik, Rossetti, Batlle, Porphyrin biosynthesis in Euglena gracilis (1981) IV An endogenous factor controlling the enzyme synthesis of porphyrinogens and its role in the treatment of some porphyrias, 13, pp. 343-353. , Int. J. Biochem | ||
| 504 | |a Keilin, Hartree, Purification of horseradish peroxidase and comparison of its properties with those of catalase and methaemoglobin (1951) Biochem. J., 49, pp. 88-104 | ||
| 504 | |a Koopmann G. E., Juknat de Geralnik A. A. and Batlle A. M. del C. Porphyrin biosynthesis in Rhodopseudomonas palustris V. Purification and characterization of porphyrinogen decarboxylase. Int. J. Biochem. (in press); Lara, Mills, Tryptophan synthetase in Euglena gracilis strain G (1972) J. Bacteriol., 110, pp. 1100-1106 | ||
| 504 | |a Lenard, Singer, Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism. (1966) Proc Natl Acad Sci U S A, 56, pp. 1828-1835 | ||
| 504 | |a Llambias, Batlle, Studies on the porphobilinogen deaminase-uroporphyrinogen cosynthetase system of cultured soya-bean cells (1971) Biochem J, 121, pp. 327-340 | ||
| 504 | |a Llambias, Batlle, Porphyrin biosynthesis VIII. Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen cosynthetase (1971) Its purification properties and the separation of its components, 227, pp. 180-191. , Biochim. biophys. Acta | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Moore, Labbe, Assays for ALA and PBG determination (1964) Clin. Chem., 10, pp. 1105-1109 | ||
| 504 | |a Ornstein, Disc. electrophoresis I (1964) Background and theory, 121, pp. 321-349. , Ann. N.Y. Acad. Sci | ||
| 504 | |a Pinto, Frisell, Correlation of the critical micelle concentrations of suffactants with their effects on a bacterial demethylase (38672) (1975) Proc. Soc. exp. Biol. Med., 148, pp. 981-985 | ||
| 504 | |a Polo, Stella, Wider, Batlle, Lenczner, Altuna, Magnin, Porfirinas en la piel de porfiria cutanea tardia por cromatografia liquida a alta presión (1983) Prensa Med Mex, 70, pp. 433-440 | ||
| 504 | |a Price, Valle, Euglena gracilis a test organism for study of zinc (1962) PLANT PHYSIOLOGY, 37, pp. 428-433 | ||
| 504 | |a Rimington, Spectral-absorption coefficients of some porphyrins in the soret-band region (1960) Biochem J, 75, pp. 620-623 | ||
| 504 | |a Rossetti, Batlle, Polypyrrole intermediates in porphyrin biosynthesis (1977) Studies with Euglena gracilis, 8, pp. 277-282. , Int. J. Biochem | ||
| 504 | |a Rossetti, Juknat de Geralnik, Batlle, Porphyrin biosynthesis in Euglena gracilis II (1977) Pyrrylmethane intermediates in the enzymic cyclotetramerization of porphobilinogen, 8, pp. 781-787. , Int. J. Biochem | ||
| 504 | |a Rossetti, Juknat de Geralnik, Kotler, Fumagalli, Batlle, Occurrence of multiple molecular forms of porphobilinogenase in diverse organisms: the minimum quaternary structure of porphobilinogenase is a protomer of one deaminase and one isomerase domain (1980) Int. J. Biochem., 12, pp. 761-767 | ||
| 504 | |a Sancovich, Batlle, Grinstein, Porphyrin biosynthesis VI (1969) Separation and purification of porphobilinogen deaminase and porphobilinogenase, an allosteric enzyme, 191, pp. 130-143. , Biochim. biophys. Acta | ||
| 504 | |a Sancovich, Ferramola, Batlle, Grinstein, Preparation of porphobilinogen (1970) Methods in Enzymology, 17 A, pp. 220-222. , H. Tabor, C.W. Tabor, Academic Press, New York | ||
| 504 | |a Sanovich, Ferramola, Batlle, Kivilevich, Grinstein, Studies on cow liver porphobilinogen deaminase (1976) Acta Physiol. Latinoam., 26, pp. 376-386 | ||
| 504 | |a Scott, Burton, Jordan, Matsumoto, Fagerness, Pryde, NMR spectroscopy as a probe for the study of enzyme-catalyzed reactions (1980) Further observations of preuroporphyrinogen, a substrate for uroporphyrinogen III cosynthetase, pp. 384-387. , J.C.S. Chem. Comm | ||
| 504 | |a Seehra, Jordan, Mechanism of action of porphobilinogen deaminase: ordered addition of the four porphobilinogen molecules in the formation of preuroporphyrinogen (1980) J. Am. chem. Soc., 102, pp. 6841-6846 | ||
| 504 | |a Singer, Structure and function of biological membranes (1971) N.Y. Academis, 145, pp. 222-240 | ||
| 504 | |a Singer, The molecular organization of membranes (1974) A. Rev. Biochem., 43, pp. 805-820 | ||
| 504 | |a Singer, Nicholson, The fluid mosaic model of the structures of cell membranes (1972) Science, 175, pp. 720-731 | ||
| 504 | |a Tigier, Batlle, Locascio, Porphyrin biosynthesis in the soybean callus tissue system II (1970) Improved purification and some properties of delta aminolaevulic dehydratase, 38, pp. 43-46 | ||
| 504 | |a Wallach, Zahler, Protein conformation in cellular membranes (1966) Proc. Natn. Acad. Sci. U.S.A, 56, pp. 1552-1559 | ||
| 520 | 3 | |a 1. 1. Porphobilinogenase (PBG-ase) from soluble and particulate fractions of Euglena gracilis was isolated and purified. 2. 2. A highly efficient method for the dissociation of the membrane-bound enzyme using the chaotropic agent SCN is described. 3. 3. The chromatographic behaviour of both soluble and particulate PBG-ase on Sephadex G-100 and Sepharose 4B was quite similar but not identical. 4. 4. Euglena gracilis PBG-ase appears to exist in an equilibrium mixture of two active species of mol. wt 50,000 and 25,000. © 1986. |l eng | |
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgements--Maria Victoria Rossetti and Alcira M. del C. Batlle hold the post of Scientific Researchers in the Argentine National Research Council (CONICET). This work was supported by grants from the CONICET, the SUBCYT, Secretaria de Salud Pfiblica del Ministerio de Bienestar Social and Banco de la Naci6n Argentina. The technical assistance of Mrs B. Riccillo de Aprea is gratefully acknowledged. | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET-FCEN, UBA), Ciudad Universitaria, Pabellon II, 4 to, Piso, 1428 Buenos Aires, Argentina | ||
| 700 | 1 | |a Lombardo, M.E. | |
| 700 | 1 | |a Juknat De Geralnik, A.A. | |
| 700 | 1 | |a Araujo, L.S. | |
| 700 | 1 | |a Del C. Batlle, A.M. | |
| 773 | 0 | |d 1986 |g v. 85 |h pp. 451-458 |k n. 2 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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