A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts
Glyphosate-based herbicides are extensively used for weed control all over the world. Therefore, it is important to investigate the putative toxic effects of these formulations which include not only glyphosate itself but also surfactants that may also be toxic. 3T3-L1 fibroblasts are a useful tool...
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| Formato: | Capítulo de libro |
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2012
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 09632caa a22010217a 4500 | ||
|---|---|---|---|
| 001 | PAPER-9375 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203917.0 | ||
| 008 | 190411s2012 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84863842719 | |
| 024 | 7 | |2 cas |a caspase 3, 169592-56-7; glyphosate, 1071-83-6; lipocortin 5, 111237-10-6; Caspase 3, 3.4.22.-; Glycine, 56-40-6; Herbicides; glyphosate, 1071-83-6 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a TIVIE | ||
| 100 | 1 | |a Martini, C.N. | |
| 245 | 1 | 2 | |a A commercial formulation of glyphosate inhibits proliferation and differentiation to adipocytes and induces apoptosis in 3T3-L1 fibroblasts |
| 260 | |c 2012 | ||
| 270 | 1 | 0 | |m Vila, M.D.C.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina; email: mvila@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Acquavella, J.F., Alexander, B.H., Mandel, J.S., Gustin, C., Baker, B., Chapman, P., Bleeke, M., Glyphosate biomonitoring for farmers and their families: results from the farm family exposure study (2004) Environ. Health Perspect., 112, pp. 321-326 | ||
| 504 | |a Benachour, N., Seralini, G.E., Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells (2009) Chem. Res. Toxicol., 22, pp. 97-105 | ||
| 504 | |a Benachour, N., Sipahutar, H., Moslemi, S., Gasnier, C., Travert, C., Seralini, G.E., Time- and dose-dependent effects of Roundup® on human embryonic and placental cells (2007) Arch. Environ. Contam. Toxicol., 53, pp. 126-133 | ||
| 504 | |a Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding (1976) Anal. Biochem., 72, pp. 248-254 | ||
| 504 | |a Dallegrave, E., Mantese, F.D., Oliveira, R.T., Andrade, A.J., Dalsenter, P.R., Langeloh, A., Pre- and postnatal toxicity of the commercial glyphosate formulation in Wistar rats (2007) Arch. Toxicol., 81, pp. 665-673 | ||
| 504 | |a De Roos, A.J., Blair, A., Rusiecki, J.A., Hoppin, J.A., Svec, M., Dosemeci, M., Sandler, D.P., Alavanja, M.C., Cancer incidence among glyphosate-exposed pesticide applicators in the agricultural health study (2005) Environ. Health Perspect., 113, pp. 49-54 | ||
| 504 | |a Dill, G.M., Cajacob, C.A., Padgette, S.R., Glyphosate-resistant crops: adoption, use and future considerations (2008) Pest Manag. Sci., 64, pp. 326-331 | ||
| 504 | |a Gianessi, L.P., Economic and herbicide use impacts of glyphosate-resistant crops (2005) Pest Manag. Sci., 61, pp. 241-245 | ||
| 504 | |a Jin, S., Zhai, B., Qiu, Z., Wu, J., Lane, M.D., Liao, K., C-Crk, a substrate of theinsulin-like growth factor-1 receptor tyrosine kinase, functions as an early signal mediator in the adipocyte differentiation process (2000) J. Biol. Chem., 275, pp. 34344-34352 | ||
| 504 | |a Liao, K., Lane, M.D., The blockade of preadipocyte differentiation by proteintyrosine phosphatase HA2 is reversed by vanadate (1995) J. Biol. Chem., 270, pp. 12123-12132 | ||
| 504 | |a Marc, J., Bellé, R., Morales, J., Cormier, P., Mulner-Lorillon, O., Formulated glyphosate activates the DNA-response checkpoint of the cell cycle leading to the prevention of G2/M transition (2004) Toxicol. Sci., 82, pp. 4364-4342 | ||
| 504 | |a Martini, C., Plaza, M.V., Vila, M.C., PKA-dependent and independent cAMP signalling in 3T3-L1 fibroblasts differentiation (2009) Mol. Cell. Endocrinol., 298, pp. 42-47 | ||
| 504 | |a Paganelli, A., Ganso, V., Acosta, H., López, S.L., Carrasco, A.E., Glyphosate-based herbicides produce teratogenic effects on vertebrates by impairing retinoic acid signaling (2010) Chem. Res. Toxicol., 23, pp. 1586-1595 | ||
| 504 | |a Patel, Y.M., Lane, M.D., Mitotic clonal expansion during preadipocyte differentiation: calpain-mediated turnover of p27 (2000) J. Biol. Chem., 275, pp. 17653-17660 | ||
| 504 | |a Peruzzo, P.J., Porta, A.A., Ronco, A.E., Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina (2008) Environ. Pollut., 156, pp. 61-66 | ||
| 504 | |a Poletta, G.L., Larriera, A., Kleinsorge, E., Mudry, M.D., Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the comet assay and the micronucleus test (2009) Mutat. Res., 672, pp. 95-102 | ||
| 504 | |a Qiu, Z., Wei, Y., Chen, N., Jiang, M., Wu, J., Liao, K., DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocytes into adipocytes (2001) J. Biol. Chem., 276, pp. 11988-11995 | ||
| 504 | |a Richard, S., Moslemi, S., Sipahutar, H., Benachour, N., Seralini, G.E., Differential effects of glyphosate and roundup on human placental cells and aromatase (2005) Environ. Health Perspect., 113, pp. 716-720 | ||
| 504 | |a Rubin, C.S., Hirsch, A., Fung, C., Rosen, O.M., Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells (1978) J. Biol. Chem., 253, pp. 7570-7578 | ||
| 504 | |a Solomon, K.R., Anadón, A., Carrasquilla, G., Cerdeira, A.L., Marshall, J., Sanin, L.H., Coca and poppy eradication in Colombia: environmental and human health assessment of aerially applied glyphosate (2007) Rev. Environ. Contam. Toxicol., 190, pp. 43-125 | ||
| 504 | |a Student, A.K., Hsu, R.Y., Lane, M.D., Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes (1980) J. Biol. Chem., 255, pp. 4745-4750 | ||
| 504 | |a Yeh, W., Bierer, B.E., McKnight, S.L., Rapamycin inhibits clonal expansion and adipogenic differentiation of 3T3-L1 cells (1995) Proc. Natl Acad. Sci. USA, 92, pp. 11086-11090 | ||
| 520 | 3 | |a Glyphosate-based herbicides are extensively used for weed control all over the world. Therefore, it is important to investigate the putative toxic effects of these formulations which include not only glyphosate itself but also surfactants that may also be toxic. 3T3-L1 fibroblasts are a useful tool to study adipocyte differentiation, this cell line can be induced to differentiate by addition of a differentiation mixture containing insulin, dexamethasone and 3-isobutyl-1-methylxanthine. We used this cell line to investigate the effect of a commercial formulation of glyphosate (GF) on proliferation, survival and differentiation. It was found that treatment of exponentially growing cells with GF for 48. h inhibited proliferation in a dose-dependent manner. In addition, treatment with GF dilution 1:2000 during 24 or 48. h inhibited proliferation and increased cell death, as evaluated by trypan blue-exclusion, in a time-dependent manner. We showed that treatment of 3T3-L1 fibroblasts with GF increased caspase-3 like activity and annexin-V positive cells as evaluated by flow cytometric analysis, which are both indicative of induction of apoptosis. It was also found that after the removal of GF, remaining cells were able to restore proliferation. On the other hand, GF treatment severely inhibited the differentiation of 3T3-L1 fibroblasts to adipocytes. According to our results, a glyphosate-based herbicide inhibits proliferation and differentiation in this mammalian cell line and induces apoptosis suggesting GF-mediated cellular damage. Thus, GF is a potential risk factor for human health and the environment. © 2012 Elsevier Ltd. |l eng | |
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a 3T3-L1 FIBROBLASTS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a DIFFERENTIATION |
| 690 | 1 | 0 | |a GLYPHOSATE FORMULATION |
| 690 | 1 | 0 | |a PROLIFERATION |
| 690 | 1 | 0 | |a CASPASE 3 |
| 690 | 1 | 0 | |a GLYPHOSATE |
| 690 | 1 | 0 | |a LIPOCORTIN 5 |
| 690 | 1 | 0 | |a ADIPOCYTE |
| 690 | 1 | 0 | |a ANTIPROLIFERATIVE ACTIVITY |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL DAMAGE |
| 690 | 1 | 0 | |a CELL DEATH |
| 690 | 1 | 0 | |a CELL DIFFERENTIATION |
| 690 | 1 | 0 | |a CELL SURVIVAL |
| 690 | 1 | 0 | |a CONCENTRATION RESPONSE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a FIBROBLAST |
| 690 | 1 | 0 | |a FLOW CYTOMETRY |
| 690 | 1 | 0 | |a MAMMAL CELL |
| 690 | 1 | 0 | |a 3T3 CELLS |
| 690 | 1 | 0 | |a ADIPOCYTES |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a CASPASE 3 |
| 690 | 1 | 0 | |a CELL DIFFERENTIATION |
| 690 | 1 | 0 | |a CELL PROLIFERATION |
| 690 | 1 | 0 | |a CELL SURVIVAL |
| 690 | 1 | 0 | |a FIBROBLASTS |
| 690 | 1 | 0 | |a GLYCINE |
| 690 | 1 | 0 | |a HERBICIDES |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MAMMALIA |
| 700 | 1 | |a Gabrielli, M. | |
| 700 | 1 | |a Vila, M.D.C. | |
| 773 | 0 | |d 2012 |g v. 26 |h pp. 1007-1013 |k n. 6 |p Toxicol. Vitro |x 08872333 |w (AR-BaUEN)CENRE-7046 |t Toxicology in Vitro | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_08872333_v26_n6_p1007_Martini |y Handle |
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