Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates
Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of s...
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| LEADER | 20518caa a22018257a 4500 | ||
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| 003 | AR-BaUEN | ||
| 005 | 20230518203907.0 | ||
| 008 | 190411s2012 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84867596646 | |
| 024 | 7 | |2 cas |a cysteine proteinase, 37353-41-6; sphingosine, 123-78-4; stearic acid, 57-11-4, 646-29-7; sulfate, 14808-79-8; Antiprotozoal Agents; Cysteine Endopeptidases, 3.4.22.-; Glycoconjugates; Immunoglobulin G; Sulfoglycosphingolipids; cruzipain, 3.4.22.- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PAIMD | ||
| 100 | 1 | |a Acosta, D.M. | |
| 245 | 1 | 0 | |a Structural and immunological characterization of sulphatides: Relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates |
| 260 | |c 2012 | ||
| 270 | 1 | 0 | |m Duschak, V.G.; Área de Bioquímica de Proteínas y Glicobiologia de Parasitos, Departamento de Investigacion, Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, ANLIS-Malbrán, Ministerio de Salud de la Nación, Av. Paseo Colón 568, Buenos Aires 1063, Argentina; email: vduschak@conicet.gov.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Moncayo, A., Ortiz, Y.M., An update on Chagas disease (human American trypanosomiasis) (2006) Ann Trop Med Parasitol, 100, pp. 663-677 | ||
| 504 | |a Duschak, V.G., Couto, A.S., Targets and patented drugs for chemotherapy of Chagas disease (2010) Frontiers in Anti-Infective Drug Discovery, pp. 323-408. , Atta-ur-Rahman FRS, Choudhary MI (eds):, United Arab Emirates: Bentham Science Publishers | ||
| 504 | |a Duschak, V.G., A decade of targets and patented drugs for chemotherapy of disease (2011) Recent Pat Anti-Infect Drug Discovery, 6, pp. 216-259 | ||
| 504 | |a (2006), Estimación cuantitativa de la Enfermedad de Chagas en las Américas Organización Panamericana (OPS) de la Salud. Ref type Pamphlet. Geneva, Department of Control of Neglected Tropical Diseases (NTD). OPS/HDM/CD: 425, Montevideo, 28; Schmunis, G.A., (2007), 102 (SUPPL. 1), pp. 75-85. , Epidemiology of Chagas disease in non-endemic countries: the role of international migration. Washington, DC, Pan American Health Organization/World Health Organization; Gascon, J., Bern, C., Pinazo, M.J., Chagas disease in Spain, the United States and other non-endemic countries (2010) Acta Trop, 115, pp. 222-227 | ||
| 504 | |a (1984), Panamerican Health Organization Status of Chagas disease in the region of Américas. Epidemiologic bulletin. Washington, DC, Pan American Health Organization; Leon, J.S., Engman, D.M., The significance of autoimmunity in the pathogenesis of Chagas heart disease (2003) Front Biosci, 8, pp. 315-322 | ||
| 504 | |a Tarleton, R.L., Parasite persistence in the etiology of Chagas disease (2001) Int J Parasitol, 31, pp. 550-554 | ||
| 504 | |a Gironés, N., Fresno, M., Etiology of Chagas disease myocarditis: autoimmunity, parasite persistence, or both? (2003) Trends Parasitol, 19, pp. 19-22 | ||
| 504 | |a Ishizuka, I., Chemistry and functional distribution of sulfoglycolipids (1997) Prog Lipid Res, 36, pp. 245-319 | ||
| 504 | |a Hirahara, Y., Tsuda, M., Wada, Y., Honke, K., cDNA cloning, genomic cloning, and tissue-specific regulation of mouse cerebroside sulfotransferase (2000) Eur J Biochem, 267, pp. 1909-1917 | ||
| 504 | |a Shao, K., Hou, Q., Go, M.L., Sulfatide-tenascin interaction mediates binding to the extracellular matrix and endocytic uptake of liposomes in glioma cells (2007) Cell Mol Life Sci, 64, pp. 506-515 | ||
| 504 | |a Aruffo, A., Kolanus, W., Walz, G., Fredman, P., Seed, B., CD62/P-selectin recognition of myeloid and tumor cell sulfatides (1991) Cell, 167, pp. 35-44 | ||
| 504 | |a Konno, A., Nunogami, K., Wada, T., Inhibitory action of sulfatide, a putative ligand for L-selectin, on B cell proliferation and Ig production (1996) Int Immunol, 8, pp. 1905-1913 | ||
| 504 | |a Kamisago, S., Iwamori, M., Tai, T., Mitamura, K., Yazaki, Y., Sugano, K., Role of sulfatides in adhesion of Helicobacter pylori to gastric cancer cells (1996) Infect Immun, 64, pp. 624-628 | ||
| 504 | |a Takahashi, T., Murakami, K., Nagakura, M., Sulfatide is required for efficient replication of influenza A virus (2008) J Virol, 82, pp. 5940-5950 | ||
| 504 | |a Honke, K., Zhang, Y., Cheng, X., Kotani, N., Taniguchi, N., Biological roles of sulfoglycolipids and pathophysiology of their deficiency (2004) Glycoconj J, 21, pp. 59-62 | ||
| 504 | |a de Lederkremer, R.M., Zingales, B., Confalonieri, A.N., Couto, A.S., Martin, N.F., Colli, W., In vivo incorporation of palmitic acid and galactose in glycolipids of Trypanosoma cruzi epimastigotes (1985) Biochem Int, 10, pp. 79-88 | ||
| 504 | |a Barreto-Bergter, E., Vermelho, A.B., Hogge, L., Gorin, P.A.J., Glycolipid components of epimastigote forms of Trypanosoma cruzi (1985) Comp Biochem Physiol B, 80, pp. 543-545 | ||
| 504 | |a Uhrig, M.L., Couto, A.S., de Lederkremer, R.M., Zingales, B., Colli, W., Metabolic labelling and partial characterization of a sulfoglycolipid in Trypanosoma cruzi trypomastigotes (1992) Carbohydr Res, 231, pp. 329-334 | ||
| 504 | |a Vermelho, A.B., Hogge, L., Barreto-Bergter, E., Isolation and characterization of a neutral glycosphingolipid from the epimastigote form of Trypanosoma mega (1986) J Protozool, 33, pp. 208-213 | ||
| 504 | |a Petry, K., Nudelman, E., Eisen, H., Hakomori, S., Sulfated lipids represent common antigens on the surface of Trypanosoma cruzi and mammalian tissues (1988) Mol Biochem Parasitol, 30, pp. 113-121 | ||
| 504 | |a Vermelho, A.B., de Meirelles, M., Pereira, M.C., Pohlentz, G., Barreto-Bergter, E., Heart muscle share common neutral glycosphingolipids with Trypanosoma cruzi (1997) Acta Trop, 64, pp. 131-143 | ||
| 504 | |a Leipelt, M., Warnecke, D., Zahringer, U., Glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants and fungi (2001) J Biol Chem, 276, pp. 33621-33629 | ||
| 504 | |a Duschak, V.G., Couto, A.S., Cruzipain, the major cysteine protease of Trypanosoma cruzi: a sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review (2009) Curr Med Chem, 16, pp. 3174-3202 | ||
| 504 | |a Barboza, M., Duschak, V.G., Fukuyama, Y., Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi. Identification of sulfated high-mannose type oligosaccharides (2005) FEBS J, 272, pp. 3803-3815 | ||
| 504 | |a Acosta, D.M., Arnaiz, M.R., Esteva, M.I., Sulfates are main targets of immune responses to cruzipain and are involved in heart damage in BALB/c immunized mice (2008) Int Immunol, 20, pp. 461-470 | ||
| 504 | |a Duschak, V.G., Riarte, A., Segura, E.L., Laucella, S.A., Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease (2001) Immunol Lett, 78, pp. 135-142 | ||
| 504 | |a Cazzulo, J.J., Franke de Cazzulo, B.M., Engel, J.C., Cannata, J.J., End products and enzyme levels of aerobic glucose fermentation in trypanosomatids (1985) Mol Biochem Parasitol, 16, pp. 329-343 | ||
| 504 | |a Barboza, M., Duschak, V.G., Cazzulo, J.J., de Lederkremer, R.M., Couto, A.S., Presence of sialic acid in N-linked oligosaccharide chains and O-linked N-acetylglucosamine in cruzipain, the major cysteine proteinase of Trypanosoma cruzi (2003) Mol Biochem Parasitol, 126, pp. 293-296 | ||
| 504 | |a Report of a WHO Expert Committee World Health Program (1991) Tech Rep Ser, 811, pp. 1-95. , World Health Organization Control of Chagas disease | ||
| 504 | |a Kuschnir, E., Sgammini, H., Castro, R., Evequoz, C., Ledesma, R., Brunetto, J., Evaluation of cardiac function by radioisotopic angiography, in patients with chronic Chagas cardiopathy (1985) Arq Bras Cardiol, 45, pp. 249-256 | ||
| 504 | |a Freeze, H.H., Yeh, R., Miller, A.L., Kornfeld, S., Structural analysis of the asparagine-linked oligosaccharides from three lysosomal enzymes of Dictyostelium discoideum. Evidence for an unusual acid-stable phosphodiester (1983) J Biol Chem, 258, pp. 14874-14879 | ||
| 504 | |a Acosta, D.M., Soprano, L.L., Ferrero, M., A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin (2011) Parasite Immunol, 33, pp. 363-370 | ||
| 504 | |a Haynes, C.A., Allegood, J.C., Park, H., Sullards, M.C., Sphingolipidomics: methods for the comprehensive analysis of sphingolipids (2009) J Chromatogr B Analyt Technol Biomed Life Sci, 877, pp. 2696-2708 | ||
| 504 | |a Bronia, D.I., Aeberhard, E.E., Montamat, E.E., Segura, E.L., Fatty acids of Trypanosoma cruzi. A comparative study of the Tulahuén, ES and Brazil strains (1980) Medicina (B Aires), 40 (SUPPL. 1), pp. 154-158 | ||
| 504 | |a Barreto-Bergter, E., Vermelho, A.B., Hartmann, R., Pohlentz, G., Klein, R.A., Egge, H., Structural characterization of neutral glycosphingolipids from Trypanosoma cruzi (1992) Mol Biochem Parasitol, 51, pp. 263-270 | ||
| 504 | |a Villas-Boas, M.H., Wait, R., Silva, R.B., Rodrigues, M.L., Barreto-Bergter, E., Ceramide glycosylation and fatty acid hydroxylation influence serological reactivity in Trypanosoma cruzi glycosphingolipids (2005) FEMS Microbiol Lett, 244, pp. 47-52 | ||
| 504 | |a Barreto-Bergter, E., Vermelho, A.B., Structures of glycolipids found in trypanosomatids: contribution to parasite functions (2010) Open Parasitol J, 4, pp. 84-97 | ||
| 504 | |a Kamisaka, Y., Marks, D.L., Pagano, R.E., Purification and characterization of UDP glucose: ceramide glucosyltransferase from rat liver golgimembranes (1996) J Biol Chem, 271, pp. 2287-2293 | ||
| 504 | |a Chou, D.K., Ilyas, A.A., Evans, J.E., Costello, C., Quarles, R.H., Jungalwala, F.B., Structure of sulfated glucuronyl glycolipids in the nervous system reacting with HNK-1 antibody and some IgM paraproteins in neuropathy (1986) J Biol Chem, 261, pp. 11717-11725 | ||
| 504 | |a Ariga, T., Kusunoki, S., Asano, K., Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia (1990) Brain Res, 519, pp. 57-64 | ||
| 504 | |a Morales-Serna, J.A., Boutureira, O., Díaz, Y., Matheu, M.I., Castillón, S., Recent advances in the glycosylation of sphingosines and ceramides (2007) Carbohydr Res, 342, pp. 1595-1612 | ||
| 504 | |a Hsu, F., Turk, J., Toward total structural analysis of cardiolipins: multiple-stage linear ion-trap mass spectrometry on the [M - 2H+3Li]+ ions (2004) J Am Soc Mass Spectrom, 15, pp. 536-546 | ||
| 504 | |a Ndao, M., Spithill, T.W., Caffrey, R., Identification of novel diagnostic serum biomarkers for Chagas' disease in asymptomatic subjects by mass spectrometric profiling (2010) J Clin Microbiol, 48, pp. 1139-1149 | ||
| 504 | |a Ndao, M., Diagnosis of parasitic diseases: old and new approaches (2009) Interdiscip Perspect Infect Dis, 2009, pp. 1-15 | ||
| 504 | |a Marks, F., Meyer, C.G., Sievertsen, J., Genotyping of Plasmodium falciparum pyrimethamine resistance by matrix-assisted laser desorption-ionization time of flight mass spectrometry (2004) Antimicrob Agents Chemother, 48, pp. 466-472 | ||
| 504 | |a Balog, C.I., Mayboroda, O.A., Wuhrer, M., HOkke, C.H., Deeler, A.M., Hensbergen, P.J., Mass spectrometric identification of aberrantly glycosylated human apolipoprotein C-III peptides in urine from Schistosoma mansoni-infected individuals (2010) Mol Cell Proteomics, 9, pp. 667-681 | ||
| 504 | |a Singh, B.N., Hayes, G.R., Lucas, J.J., Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function (2009) Glycoconj J, 26, pp. 3-17 | ||
| 504 | |a Kohriyama, T., Ariga, T., Yu, R.K., Preparation and characterization of antibodies against a sulfated glucuronic acid-containing glycosphingolipid (1988) J Neurochem, 51, pp. 869-877 | ||
| 504 | |a Avila, J.L., Rojas, M., Carrasco, H., Elevated levels of antibodies against sulfatide are present in all chronic chagasic and dilated cardiomyopathy sera (1993) Clin Exp Immunol, 92, pp. 460-465 | ||
| 504 | |a Petry, K., Van Voorhis, W.C., Antigens of Trypanosoma cruzi that mimic mammalian nervous tissues: investigations of their role in the autoimmune pathophysiology of chronic Chagas' disease (1991) Res Immunol, 142, pp. 151-156 | ||
| 504 | |a Feldman, S., García, G., Svetaz, M.J., Evidence that antisulfatide autoantibodies from rats experimentally infected with Trypanosoma cruzi bind to homologous neural tissue (1999) Parasitol Res, 85, pp. 446-451 | ||
| 504 | |a Avila, J.L., Rojas, M., Acosta, A., Glycoinositol phospholipids from American Leishmania and Trypanosoma spp.: partial characterization of the glycan cores and the human humoral immune response to them (1991) J Clin Microbiol, 29, pp. 2305-2312 | ||
| 504 | |a García, R., Avila, J.L., Rojas, M., Martínez, A., García, W., Bergel, P., Anti-sulfatide antibody titers in patients with chronic Chagas disease and other forms of cardiopathy (1998) Rev Panam Salud Publica, 3, pp. 249-256 | ||
| 504 | |a Petry, K., Voisin, P., Baltz, T., Labouesse, J., Epitopes common to trypanosomes (T. cruzi, T. dionisii and T. vespertilionis (Schizotrypanum]: astrocytes and neurons (1987) J Neuroimmunol, 16, pp. 237-252 | ||
| 504 | |a Avila, J.L., Molecular mimicry between Trypanosoma cruzi and host nervous tissues (1992) Acta Cient Venez, 43, pp. 330-340 | ||
| 504 | |a Avila, J.L., Rojas, M., A Galactosyl(al-3)Mannose epitope on phospholipids of Leishmania mexicana and L. Braziliensis is recognized by Trypanosomatid-Infected Human Sera (1990) J Clin Microbiol, 28, pp. 1530-1537 | ||
| 504 | |a Avila, J.L., Rojas, M., Elevated cerebroside antibody levels in human visceral and cutaneous leishmaniasis, Trypanosoma rangeli infection, and chronic Chagas' disease (1990) Am J Trop Med Hyg, 43, pp. 52-60 | ||
| 504 | |a Avila, J.L., Rojas, M., Towbin, H., Serological activity against galactosyl-alpha(1-3)galactose in sera from patients with several kinetoplastida infections (1988) J Clin Microbiol, 26, pp. 126-132 | ||
| 504 | |a Avila, J.L., Rojas, M., García, L., Persistence of elevated levels of galactosyl-alpha(1-3)galactose antibodies in sera from patients cured of visceral leishmaniasis (1988) J Clin Microbiol, 26, pp. 1842-1847 | ||
| 504 | |a Avila, J.L., Rojas, M., Rodas, A., Convit, J., Parasitic oligosaccharide residues recognized by patients with mucocutaneous and localized cutaneous leishmaniasis (1992) Am J Trop Med Hyg, 47, pp. 284-290 | ||
| 504 | |a Avila, J.L., Rojas, M., Velazquez-Avila, G., Characterization of a natural human antibody with anti-galactosyl(alpha 1-2)galactose specificity that is present at high titers in chronic Trypanosoma cruzi infection (1992) Am J Trop Med Hyg, 47, pp. 413-421 | ||
| 504 | |a Avila, J.L., Rojas, M., Avila, A., Increase in asialoganglioside- and monosialoganglioside-reactive antibodies in chronic Chagas' disease patients (1998) Am J Trop Med Hyg, 58, pp. 338-342 | ||
| 504 | |a Didoli, G.L., Davila, H.O., Feldman, S., di Masso, R., Revelli, S.S., Bottasso, O.A., Protected Trypanosoma cruzi infection in rats born to mothers receiving interferon-gamma during gestation is associated with a decreased intramacrophage parasite growth and preferential synthesis of specific IgG2b antibodies (2000) Int J Immunopharmacol, 22, pp. 45-55 | ||
| 504 | |a Brodskyn, C., Silva, A., Takehara, H., Mota, I., IgG subclasses responsible for immune clearance in mice infected with Trypanosoma cruzi (1989) Immunol Cell Biol, 67, pp. 343-348 | ||
| 504 | |a Schmitz, J.E., Kuroda, M.J., Santra, S., Effect of humoral immune responses on controlling viremia during primary infection of rhesus monkeys with simian immunodeficiency virus (2003) J Virol, 77, pp. 2165-2173 | ||
| 504 | |a Aucan, C., Traoré, Y., Tall, F., High immunoglobulin G2 (IgG2) and low IgG4 levels are associated with human resistance to Plasmodium falciparum malaria (2000) Infect Immun, 68, pp. 1252-1258 | ||
| 504 | |a Luquetti, A.O., Rassi, A., Tratamiento específico de la enfermedad de Chagas en la fase cronica: criterios de cura convencionales: xenodiagnostico, hemocultivo, serologia (1998) Rev Patol Trop, 27 (SUPPL.), p. 37 | ||
| 520 | 3 | |a Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures - composed mainly of sphingosine as the long chain base acylated with stearic acid - have been determined for the first time in Trypanosoma cruzi epimastigotes by UV-MALDI-TOF-MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C-terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross-reactivity maps to the C-terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate-containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease. © 2012 Blackwell Publishing Ltd. |l eng | |
| 593 | |a Instituto Nacional de Parasitología Dr Mario Fatala Chaben, ANLIS-Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina | ||
| 593 | |a CIHIDECAR(CONICET), Depto de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CRUZIPAIN ANTIBODIES |
| 690 | 1 | 0 | |a SULPHATE MOIETIES |
| 690 | 1 | 0 | |a SULPHATIDES |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a UV-MALDI-TOF-MS |
| 690 | 1 | 0 | |a CERAMIDE |
| 690 | 1 | 0 | |a CRUZIPAIN |
| 690 | 1 | 0 | |a CYSTEINE PROTEINASE |
| 690 | 1 | 0 | |a EPITOPE |
| 690 | 1 | 0 | |a GLYCOCONJUGATE |
| 690 | 1 | 0 | |a GLYCOLIPID |
| 690 | 1 | 0 | |a IMMUNOGLOBULIN G2 |
| 690 | 1 | 0 | |a SPHINGOSINE |
| 690 | 1 | 0 | |a STEARIC ACID |
| 690 | 1 | 0 | |a SULFATE |
| 690 | 1 | 0 | |a SULFATIDE |
| 690 | 1 | 0 | |a SULPHATED DIHEXOSYLCERAMIDE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CARBOXY TERMINAL SEQUENCE |
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a CHEMICAL STRUCTURE |
| 690 | 1 | 0 | |a CLINICAL ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CROSS REACTION |
| 690 | 1 | 0 | |a EPIMASTIGOTE |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RABBIT |
| 690 | 1 | 0 | |a STRUCTURE ANALYSIS |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ULTRAVIOLET RADIATION |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIPROTOZOAL AGENTS |
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a CROSS REACTIONS |
| 690 | 1 | 0 | |a CYSTEINE ENDOPEPTIDASES |
| 690 | 1 | 0 | |a ENZYME-LINKED IMMUNOSORBENT ASSAY |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GLYCOCONJUGATES |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a IMMUNOGLOBULIN G |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MIDDLE AGED |
| 690 | 1 | 0 | |a RABBITS |
| 690 | 1 | 0 | |a SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION |
| 690 | 1 | 0 | |a SULFOGLYCOSPHINGOLIPIDS |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Soprano, L.L. | |
| 700 | 1 | |a Ferrero, M.R. | |
| 700 | 1 | |a Esteva, M.I. | |
| 700 | 1 | |a Riarte, A. | |
| 700 | 1 | |a Couto, A.S. | |
| 700 | 1 | |a Duschak, V.G. | |
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