A novel stimulator of protein phosphorylation in Neurospora crassa
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or...
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| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1987
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers. |
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| Bibliografía: | Nimmo, H.G., Cohen, P., Hormonal control of protein phosphorylation (1977) Adv Cycl Nuc Res, 8, pp. 145-251 Krebs, E.G., Beavo, J.A., Phosphorylation-dephosphorylation of enzymes (1979) Ann Rev Biochem, 48, pp. 923-959 Krebs, E.G., Historical perspectives on protein phosphorylation and a classification system for protein kinaes (1983) Phil-Trans-R. R Soc London, 302, pp. 3-1 Judewicz, N.D., Glikin, G.C., Torres, H.N., Protein kinase activities in Neurospora crassa (1981) Arch Biochem Biophys, 206, pp. 87-92 Glikin, G.C., Judewicz, N.D., Torres, H.N., Protein kinase activities in Neurospora crassa- Molecular Properties (1982) Mol Cell Biochem, 46, pp. 121-126 Powers, P.A., Pall, M.L., Cyclic AMP-dependent protein kinase of Neurospora crassa (1980) Biochem Biophys Res Comm, 95, pp. 701-706 Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 Glynn, I.M., Chappell, J.B., A simple method for the preparation of (32P)-labelled adenosine triphosphate of high specific activity (1964) Biochem J, 90, pp. 147-149 Téllez-Iñón, M.T., Ulloa, R.M., Glikin, G.C., Torres, H.N., Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin (1985) Biochem J, 232, pp. 425-430 |
| ISSN: | 03008177 |
| DOI: | 10.1007/BF00230146 |