A novel stimulator of protein phosphorylation in Neurospora crassa

An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or...

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Autor principal: Ulloa, R.M
Otros Autores: Glikin, G.C, Téllez-Ĩnón, M.T, Torres, H.N, Judewicz, N.D
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Kluwer Academic Publishers 1987
Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a protein kinase, 9026-43-1; Fungal Proteins; Protein Kinases, EC 2.7.1.37 
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030 |a MCBIB 
100 1 |a Ulloa, R.M. 
245 1 2 |a A novel stimulator of protein phosphorylation in Neurospora crassa 
260 |b Kluwer Academic Publishers  |c 1987 
270 1 0 |m Judewicz, N.D.; INGEBI-CONICET, Obligado 2490, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Nimmo, H.G., Cohen, P., Hormonal control of protein phosphorylation (1977) Adv Cycl Nuc Res, 8, pp. 145-251 
504 |a Krebs, E.G., Beavo, J.A., Phosphorylation-dephosphorylation of enzymes (1979) Ann Rev Biochem, 48, pp. 923-959 
504 |a Krebs, E.G., Historical perspectives on protein phosphorylation and a classification system for protein kinaes (1983) Phil-Trans-R. R Soc London, 302, pp. 3-1 
504 |a Judewicz, N.D., Glikin, G.C., Torres, H.N., Protein kinase activities in Neurospora crassa (1981) Arch Biochem Biophys, 206, pp. 87-92 
504 |a Glikin, G.C., Judewicz, N.D., Torres, H.N., Protein kinase activities in Neurospora crassa- Molecular Properties (1982) Mol Cell Biochem, 46, pp. 121-126 
504 |a Powers, P.A., Pall, M.L., Cyclic AMP-dependent protein kinase of Neurospora crassa (1980) Biochem Biophys Res Comm, 95, pp. 701-706 
504 |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 
504 |a Glynn, I.M., Chappell, J.B., A simple method for the preparation of (32P)-labelled adenosine triphosphate of high specific activity (1964) Biochem J, 90, pp. 147-149 
504 |a Téllez-Iñón, M.T., Ulloa, R.M., Glikin, G.C., Torres, H.N., Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin (1985) Biochem J, 232, pp. 425-430 
520 3 |a An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers.  |l eng 
593 |a Instituto, de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI) CONICET, Obligado 2490, Buenos Aires, 1428, Argentina 
593 |a Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a NEUROSPORA CRASSA 
690 1 0 |a PROTEIN KINASE REGULATION 
690 1 0 |a FUNGAL PROTEIN 
690 1 0 |a PROTEIN KINASE 
690 1 0 |a ARTICLE 
690 1 0 |a ENZYME ACTIVATION 
690 1 0 |a ENZYMOLOGY 
690 1 0 |a GEL CHROMATOGRAPHY 
690 1 0 |a GROWTH, DEVELOPMENT AND AGING 
690 1 0 |a KINETICS 
690 1 0 |a METABOLISM 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a NEUROSPORA 
690 1 0 |a NEUROSPORA CRASSA 
690 1 0 |a PHOSPHORYLATION 
690 1 0 |a CHROMATOGRAPHY, GEL 
690 1 0 |a ENZYME ACTIVATION 
690 1 0 |a FUNGAL PROTEINS 
690 1 0 |a KINETICS 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a NEUROSPORA 
690 1 0 |a NEUROSPORA CRASSA 
690 1 0 |a PHOSPHORYLATION 
690 1 0 |a PROTEIN KINASES 
700 1 |a Glikin, G.C. 
700 1 |a Téllez-Ĩnón, M.T. 
700 1 |a Torres, H.N. 
700 1 |a Judewicz, N.D. 
773 0 |d Kluwer Academic Publishers, 1987  |g v. 77  |h pp. 11-17  |k n. 1  |p Mol Cell Biochem  |x 03008177  |w (AR-BaUEN)CENRE-3498  |t Molecular and Cellular Biochemistry 
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856 4 0 |u https://doi.org/10.1007/BF00230146  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa  |y Handle 
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