PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation
Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). Howe...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2009
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 09983caa a22011057a 4500 | ||
|---|---|---|---|
| 001 | PAPER-8979 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203850.0 | ||
| 008 | 190411s2009 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-57749200703 | |
| 024 | 7 | |2 cas |a bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide, 127243-85-0; 1-Methyl-3-isobutylxanthine, 28822-58-4; 8-cyclopentyl-1,3-dimethylxanthine, 35873-49-5; Cyclic AMP, 60-92-4; Cyclic AMP-Dependent Protein Kinases, EC 2.7.11.11; Dexamethasone, 50-02-2; H 89, 127243-85-0; Insulin, 11061-68-0; Isoquinolines; Protein Kinase Inhibitors; Sulfonamides; Theophylline, 58-55-9 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MCEND | ||
| 100 | 1 | |a Martini, C.N. | |
| 245 | 1 | 0 | |a PKA-dependent and independent cAMP signaling in 3T3-L1 fibroblasts differentiation |
| 260 | |c 2009 | ||
| 270 | 1 | 0 | |m Vila, M.d.C.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: mvila@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding (1976) Analytical Biochemistry, 72, pp. 248-254 | ||
| 504 | |a Clegg, C.H., Abrahamsen, M.S., Degen, J.L., Morris, D.R., McKnight, G.S., Cyclic AMP-dependent protein kinase controls basal gene activity and steroidogenesis in Y1 adrenal tumor cells (1992) Biochemistry, 31, pp. 3720-3726 | ||
| 504 | |a Chin, E.C., Abayasekara, D.R.E., Progesterone secretion by luteinizing human granulosa cells: a possible cAMP-dependent but PKA-independent mechanism involved in its regulation (2004) Journal of Endocrinology, 183, pp. 51-60 | ||
| 504 | |a Gregoire, F.M., Smas, C.M., Sul, H.S., Understanding adipocyte differentiation (1998) Physiological Reviews, 78, pp. 783-809 | ||
| 504 | |a Holz, G.G., Kang, G., Harbeck, M., Roe, M.W., Chepurny, O.G., Cell physiology of cAMP sensor Epac (2006) Journal of Physiology, 577 (PART 1), pp. 5-15 | ||
| 504 | |a Jin, S., Zhai, B., Qiu, Z., Wu, J., Lane, M.D., Liao, K., c-Crk, a substrate of the insulin-like growth factor-1 receptor tyrosine kinase, functions as an early signal mediator in the adipocyte differentiation process (2000) Journal of Biological Chemistry, 275, pp. 34344-34352 | ||
| 504 | |a Kanda, Y., Watanabe, Y., Adrenaline increases glucose transport via a Rap1-p38MAPK pathway in rat vascular smooth muscle cells (2007) British Journal of Pharmacology, 151, pp. 476-482 | ||
| 504 | |a Kato, Y., Ozaki, N., Yamada, T., Miura, Y., Oiso, Y., H-89 potentiates adipogenesis in 3T3-L1 cells by activating insulin signaling independently of protein kinase A (2007) Life Science, 80, pp. 476-483 | ||
| 504 | |a Kawasaki, H., Springett, G.M., Mochizuki, N., Toki, S., Nakaya, M., Matsuda, M., Housman, D.E., Graybiel, A.M., A family of cAMP-binding proteins that directly activate Rap1 (1998) Science, 282, pp. 2275-2279 | ||
| 504 | |a Lean, M.E., Pathophysiology of obesity (2000) The Proceedings of the Nutrition Society, 59, pp. 331-336 | ||
| 504 | |a Liao, K., Lane, M.D., The blockade of preadipocyte differentiation by protein-tyrosine phosphatase HA2 is reversed by vanadate (1995) Journal Biological Chemistry, 270, pp. 12123-12132 | ||
| 504 | |a Mellon, P.L., Clegg, C.H., Correll, L.A., McKnight, G.S., Regulation of transcription by cyclic AMP-dependent protein kinase. (1989) Proceedings of the National Academy of Sciences, United States of America, 86, pp. 4887-4891 | ||
| 504 | |a Patel, Y.M., Lane, M.D., Mitotic clonal expansion during preadipocyte differentiation: calpain-mediated turnover of p27 (2000) Journal of Biological Chemistry, 275, pp. 17653-17660 | ||
| 504 | |a Qiu, Z., Wei, Y., Chen, N., Jiang, M., Wu, J., Liao, K., DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocytes into adipocytes (2001) Journal of Biological Chemistry, 276, pp. 11988-11995 | ||
| 504 | |a Rehmann, H., Das, J., Knipscheer, P., Wittinghofer, A., Bos, J.L., Structure of the cyclic-AMP-responsive exchange factor Epac2 in its auto-inhibited state (2006) Nature, 439, pp. 625-628 | ||
| 504 | |a Rubin, C.S., Hirsch, A., Fung, C., Rosen, O.M., Development of hormone receptors and hormonal responsiveness in vitro. Insulin receptors and insulin sensitivity in the preadipocyte and adipocyte forms of 3T3-L1 cells (1978) Journal of Biological Chemistry, 253, pp. 7570-7578 | ||
| 504 | |a Student, A.K., Hsu, R.Y., Lane, M.D., Induction of fatty acid synthetase synthesis in differentiating 3T3-L1 preadipocytes (1980) Journal of Biological Chemistry, 255, pp. 4745-4750 | ||
| 504 | |a Varone, C.L., Giono, L.E., Ochoa, A., Zakin, M.M., Cánepa, E.T., Transcriptional regulation of 5-aminolevulinate synthase by phenobarbital and cAMP-dependent protein kinase (1999) Archives of Biochemistry and Biophysics, 372, pp. 261-270 | ||
| 504 | |a Xu, J., Liao, K., Protein kinase B/AKT 1 plays a pivotal role in insulin-like growth factor-1 receptor signaling induced 3T3-L1 adipocyte differentiation (2004) Journal of Biological Chemistry, 279, pp. 35914-35922 | ||
| 504 | |a Yeh, W., Bierer, B.E., McKnight, S.L., Rapamycin inhibits clonal expansion and adipogenic differentiation of 3T3-L1 cells (1995) Proceedings of the National Academy of Sciences, United States of America, 92, pp. 11086-11090 | ||
| 504 | |a Yin, F., Wang, Y.Y., Du, J.H., Li, C., Lu, Z.Z., Han, C., Zhang, Y.Y., Noncanonical cAMP pathway and p38 MAPK mediate beta2-adrenergic receptor-induced IL-6 production in neonatal mouse cardiac fibroblasts (2006) Journal of Molecular and Cellular Cardiology, 40, pp. 384-393 | ||
| 520 | 3 | |a Adipogenesis is stimulated in 3T3-L1 fibroblast by a combination of insulin, dexamethasone, and methylisobutylxanthine (MIX). Mitotic clonal expansion (MCE) precedes differentiation of 3T3-L1 fibroblast to adipocytes. MIX increases cAMP content, which is the activator of protein kinase A (PKA). However, PKA-independent cAMP signaling has also been described. In this paper, it was found that H89, an inhibitor of PKA, was able to block MCE but not differentiation of 3T3-L1 fibroblast. Consistently, MCE did not occur in the absence of MIX in the differentiation mixture but was recovered by overexpression of a catalytic subunit of PKA. In addition, the transfection of 3T3-L1 fibroblast with a dominant-negative mutant of PKA inhibited MCE. On the other hand, differentiation of 3T3-L1 fibroblast to adipocytes did not occur when MIX was not present in the differentiation mixture and it could not be recovered by overexpression of a catalytic subunit of PKA. Differentiation was restored by addition of either dibutyryl-cAMP (db-cAMP) or 8 CPT-2 Me-cAMP. The latter activates cAMP-EPAC but not PKA signaling. These results indicate that cAMP-PKA-independent signaling, is required for 3T3-L1 fibroblasts differentiation to adipocytes and MIX signaling through cAMP-PKA is necessary for MCE, although MCE is not essential for adipogenesis. © 2008 Elsevier Ireland Ltd. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas and Universidad de Buenos Aires, Argentina. | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a 3T3-L1 FIBROBLAST |
| 690 | 1 | 0 | |a ADIPOGENESIS |
| 690 | 1 | 0 | |a CAMP |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a METHYLISOBUTYLXANTHINE (MIX) |
| 690 | 1 | 0 | |a MITOTIC CLONAL EXPANSION |
| 690 | 1 | 0 | |a PKA |
| 690 | 1 | 0 | |a 8 (4 CHLOROPHENYLTHIO) 2' O METHYLADENOSINE 3',5' CYCLIC MONPHOSPHATE |
| 690 | 1 | 0 | |a BUCLADESINE |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a CYCLIC AMP DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ADIPOCYTE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CATALYSIS |
| 690 | 1 | 0 | |a CELL DIFFERENTIATION |
| 690 | 1 | 0 | |a FIBROBLAST |
| 690 | 1 | 0 | |a GENETIC TRANSFECTION |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a 1-METHYL-3-ISOBUTYLXANTHINE |
| 690 | 1 | 0 | |a 3T3-L1 CELLS |
| 690 | 1 | 0 | |a ADIPOGENESIS |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a CELL DIFFERENTIATION |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a CYCLIC AMP-DEPENDENT PROTEIN KINASES |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a FIBROBLASTS |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a ISOQUINOLINES |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MITOSIS |
| 690 | 1 | 0 | |a PROTEIN KINASE INHIBITORS |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a SULFONAMIDES |
| 690 | 1 | 0 | |a THEOPHYLLINE |
| 700 | 1 | |a Plaza, M.V. | |
| 700 | 1 | |a Vila, M.d.C. | |
| 773 | 0 | |d 2009 |g v. 298 |h pp. 42-47 |k n. 1-2 |p Mol. Cell. Endocrinol. |x 03037207 |w (AR-BaUEN)CENRE-6138 |t Molecular and Cellular Endocrinology | |
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