Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing...
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2009
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| LEADER | 19545caa a22015257a 4500 | ||
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| 100 | 1 | |a Schiaffino, María Romina | |
| 245 | 1 | 0 | |a Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status |
| 260 | |c 2009 | ||
| 270 | 1 | 0 | |m Izaguirre, I.; Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; email: iri@ege.fcen.uba.ar |
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| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing during two consecutive austral summers (2003-2004). Analyses of the gels identified a total of 230 bands spread across 57 different positions. Among those bands, 14 were shared between lakes from Hope Bay and Potter Peninsula, 17 were observed only in particular lakes, and 17 were registered both years in the same lake. We successfully reamplified and sequenced 43 bands located in 36 different positions belonging to Bacteroidetes, Actinobacteria, Betaproteobacteria and Cyanobacteria. The closest matches for 63% of the sequenced bands were from Antarctic or from other cold environment clones and sequences already in the databases, suggesting the widespread dominance of microbial communities adapted to cold habitats. The results of the multivariate analyses (Cluster Analysis and CCA) indicated that the nutrient status of the lake influences the bacterioplankton assemblages. © 2009 Springer-Verlag. |l eng | |
| 536 | |a Detalles de la financiación: REN2002-11396-E/ANT | ||
| 536 | |a Detalles de la financiación: SB2001-0166, REN2001-2120/MAR | ||
| 536 | |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, PICT 04440, PICT 32732 | ||
| 536 | |a Detalles de la financiación: Acknowledgments The Antarctic expeditions were supported by the “Instituto Antártico Argentino (DNA)”, in the framework of a cooperative project between this institution, UBA and ICM-CSIC. This work was Wnanced by grants of the Argentinean Funds for Technical and ScientiWc Investigation (FONCYT, PICT 04440 and PICT 32732), and by the Spanish projects MIXANTAR (REN2002-11396-E/ANT) and MICRODIFF (DGICYT REN2001-2120/MAR) grant SB2001-0166 from the Spanish MECyD. We wish to thank the members of the Esperanza Station for the logistic support, P. Rodriguez and E. Moreno-Díaz for their assistance with the Weld work, and Dr. Ramon Massana for general support. We also thank the reviewers for their valuable suggestions on the manuscript. | ||
| 593 | |a Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a CONICET (Consejo Nacional de Investigación Científica y Técnica), Buenos Aires, Argentina | ||
| 593 | |a IIB-INTECH, Chascomús, Argentina | ||
| 593 | |a Departament de Biología Marina i Oceanografia, Institut de Ciències Del Mar-CSIC, Barcelona, Catalonia, Spain | ||
| 593 | |a PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Tucumán, Argentina | ||
| 690 | 1 | 0 | |a 16S RRNA GENE |
| 690 | 1 | 0 | |a BACTERIOPLANKTON |
| 690 | 1 | 0 | |a DENATURING GRADIENT GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a HOPE BAY (ANTARCTIC PENINSULA) |
| 690 | 1 | 0 | |a MARITIME ANTARCTIC LAKES |
| 690 | 1 | 0 | |a POTTER PENINSULA (KING GEORGE ISLAND) |
| 690 | 1 | 0 | |a BACTERIOPLANKTON |
| 690 | 1 | 0 | |a BACTERIUM |
| 690 | 1 | 0 | |a CLUSTER ANALYSIS |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a CYANOBACTERIUM |
| 690 | 1 | 0 | |a DOMINANCE |
| 690 | 1 | 0 | |a ELECTROKINESIS |
| 690 | 1 | 0 | |a GEL |
| 690 | 1 | 0 | |a GENE |
| 690 | 1 | 0 | |a MICROBIAL COMMUNITY |
| 690 | 1 | 0 | |a RNA |
| 690 | 1 | 0 | |a TROPHIC STATUS |
| 690 | 1 | 0 | |a ANTARCTIC PENINSULA |
| 690 | 1 | 0 | |a ANTARCTICA |
| 690 | 1 | 0 | |a HOPE BAY |
| 690 | 1 | 0 | |a KING GEORGE ISLAND |
| 690 | 1 | 0 | |a POTTER PENINSULA |
| 690 | 1 | 0 | |a SOUTH SHETLAND ISLANDS |
| 690 | 1 | 0 | |a WEST ANTARCTICA |
| 690 | 1 | 0 | |a ACTINOBACTERIA |
| 690 | 1 | 0 | |a BACTEROIDETES |
| 690 | 1 | 0 | |a BETAPROTEOBACTERIA |
| 690 | 1 | 0 | |a CYANOBACTERIA |
| 700 | 1 | |a Unrein, Fernando | |
| 700 | 1 | |a Gasol, J.M. | |
| 700 | 1 | |a Farias, M.E. | |
| 700 | 1 | |a Estevez, C. | |
| 700 | 1 | |a Balagué, V. | |
| 700 | 1 | |a Izaguirre, I. | |
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