Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

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Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) ir...

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Detalles Bibliográficos
Autor principal: Ibañez, I.L
Otros Autores: Bracalente, C., Molinari, B.L, Palmieri, M.A, Policastro, L., Kreiner, A.J, Burlón, Alejandro Alfredo, Valda, A., Navalesi, D., Davidson, Jorge, Davidson, Miguel, Vázquez, M., Ozafrán, M., Durán, H.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2009
Materias:
GENES
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-67649525972 
024 7 |2 cas  |a Biological Markers; H2AFX protein, human; Histones; Lithium, 7439-93-2; Protons; Radioisotopes 
030 |a IOBPD 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Ibañez, I.L. 
245 1 0 |a Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams 
260 |c 2009 
270 1 0 |m Durán, H.; Comisión Nacional de Energía Atómica, San Martín, Argentina; email: hduran@cnea.gov.ar 
504 |a Ivanov, V.N., Zhou, H., Hei, T.K., Sequential treatment by ionizing radiation and sodium arsenite dramatically accelerates TRAIL-mediated apoptosis of human melanoma cells (2007) Cancer Res, 67, pp. 5397-5407 
504 |a Schulz-Ertner, D., Jäkel, O., Schlegel, W., Radiation therapy with charged particles (2006) Semin Radiat Oncol, 16, pp. 249-259 
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504 |a Van Gent, D.C., Hoeijmakers, J.H., Kanaar, R., Chromosomal stability and the DNA double-stranded break connection (2001) Nat Rev Genet, 2, pp. 196-206 
504 |a Rogakou, E.P., Boon, C., Redon, C., Megabase chromatin domains involved in DNA double-strand breaks in vivo (1999) J Cell Biol, 146, pp. 905-916 
504 |a Tanaka, T., Huang, X., Halicka, H.D., Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents (2007) Cytometry A, 71, pp. 648-661 
504 |a Burma, S., Chen, B.P., Murphy, M., ATM phosphorylates histone H2AX in response to DNA double-strand breaks (2001) J Biol Chem, 276, pp. 42462-42467 
504 |a Rothkamm, K., Löbrich, M., Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses (2003) Proc Natl Acad Sci U S A, 100, pp. 5057-5062 
504 |a Kinner, A., Wu, W., Staudt, C., γ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin (2008) Nucleic Acid Res, 36, pp. 5678-5694 
504 |a Ismail, I.H., Wadhra, T.I., Hammarsten, O., An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans (2007) Nucleic Acids Res, 35, pp. e36 
504 |a Desai, N., Davis, E., O'Neill, P., Immunofluorescence detection of clustered γ-H2AX foci induced by HZE-particle radiation (2005) Radiat Res, 164, pp. 518-522 
504 |a Schuff, J., Policastro, L., Durán, H., Relative biological effectiveness measurements of low energy protons and lithium beams on tumor cells (2002) Nucl Instrum Methods Phys Res B, 187, pp. 345-353 
504 |a International Atomic Energy Agency, (2000) Absorbed dose determination in external beam radiotherapy, , IAEA, Vienna Technical Report Series No. 398 
504 |a Warters, R.L., Adamson, P.J., Pond, C.D., Melanoma cells express elevated levels of phosphorylated histone H2AX foci (2005) J Invest Dermatol, 124, pp. 807-817 
504 |a Wasco, M.J., Pu, R.T., Yu, L., Expression of gamma-H2AX in melanocytic lesions (2008) Hum Pathol, 39, pp. 1614-1620 
504 |a Pilch, D.R., Sedelnikova, O.A., Redon, C., Characteristics of γ-H2AX foci at DNA double-strand breaks sites (2003) Biochem Cell Biol, 81, pp. 123-129 
504 |a Olive, P.L., Banáth, J.P., Phosphorylation of histone H2AX as a measure of radiosensitivity (2004) Int J Radiat Oncol Biol Phys, 58, pp. 331-335 
504 |a Leatherbarrow, E.L., Harper, J.V., Cucinotta, F.A., Induction and quantification of γ-H2AX foci following low and high LET-irradiation (2006) Int J Radiat Biol, 82, pp. 111-118 
504 |a Asaithamby, A., Uematsu, N., Chatterjee, A., Repair of HZE-particle-induced DNA double-strand breaks in normal human fibroblasts (2008) Radiat Res, 169, pp. 437-446 
504 |a Costes, S.V., Boissière, A., Ravani, S., Imaging features that discriminate between foci induced by high- and low-LET radiation in human fibroblasts (2006) Radiat Res, 165, pp. 505-515 
504 |a Bewersdorf, J., Bennett, B.T., Knight, K.L., H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopy (2006) Proc Natl Acad Sci U S A, 103, pp. 18137-18142 
504 |a Jakob, B., Scholz, M., Taucher-Scholz, G., Biological imaging of heavy charged-particle tracks (2003) Radiat Res, 159, pp. 676-684 
504 |a MacPhail, S.H., Banáth, J.P., Yu, Y., Cell cycle-dependent expression of phosphorylated histone H2AX: Reduced expression in unirradiated but not X-irradiated G1-phase cells (2003) Radiat Res, 159, pp. 759-767 
504 |a Kruhlak, M.J., Celeste, A., Dellaire, G., Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks (2006) J Cell Biol, 172, pp. 823-834 
504 |a Bakkenist, C.J., Kastan, M.B., DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation (2003) Nature, 421, pp. 499-506 
504 |a Aten, J.A., Stap, J., Krawczyk, P.M., Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains (2004) Science, 303, pp. 92-95 
504 |a Dikomey, E., Borgmann, K., Brammer, I., Molecular mechanisms of individual radiosensitivity studied in normal diploid human fibroblasts (2003) Toxicology, 193, pp. 125-135 
504 |a Klokov, D., MacPhail, S.M., Banáth, J.P., Phosphorylated histone H2AX in relation to cell survival in tumor cells and xenografts exposed to single and fractionated doses of X-rays (2006) Radiother Oncol, 80, pp. 223-229 
506 |2 openaire  |e Política editorial 
520 3 |a Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved.  |l eng 
593 |a Comisión Nacional de Energía Atómica, San Martín, Argentina 
593 |a Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina 
593 |a Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 
593 |a Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Argentina 
650 1 7 |2 spines  |a GENES 
690 1 0 |a ΓH2AX 
690 1 0 |a DNA DSB 
690 1 0 |a IONIZING RADIATION 
690 1 0 |a LET 
690 1 0 |a MELANOMA CELLS 
690 1 0 |a CLONOGENIC ASSAYS 
690 1 0 |a DNA DOUBLE STRAND BREAKS 
690 1 0 |a DNA DSB 
690 1 0 |a GAMMA SOURCE 
690 1 0 |a HIGH LINEAR ENERGY TRANSFERS 
690 1 0 |a IN-CELL 
690 1 0 |a LET 
690 1 0 |a LET RADIATION 
690 1 0 |a LINEAR-QUADRATIC MODELS 
690 1 0 |a LOW-LET RADIATION 
690 1 0 |a MELANOMA CELLS 
690 1 0 |a MONOENERGETIC PROTONS 
690 1 0 |a PHOSPHORYLATED HISTONE 
690 1 0 |a POSTIRRADIATION 
690 1 0 |a SURVIVAL CURVES 
690 1 0 |a SURVIVING FRACTIONS 
690 1 0 |a CELL MEMBRANES 
690 1 0 |a DNA 
690 1 0 |a ENERGY TRANSFER 
690 1 0 |a IONIZING RADIATION 
690 1 0 |a LITHIUM 
690 1 0 |a NUCLEIC ACIDS 
690 1 0 |a PHOSPHORYLATION 
690 1 0 |a PROTON IRRADIATION 
690 1 0 |a PROTONS 
690 1 0 |a RADIATION SHIELDING 
690 1 0 |a RADIOACTIVITY 
690 1 0 |a LIGHTING FIXTURES 
690 1 0 |a DOUBLE STRANDED DNA 
690 1 0 |a HISTONE H2AX 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ARTICLE 
690 1 0 |a CANCER CELL CULTURE 
690 1 0 |a CANCER RADIOTHERAPY 
690 1 0 |a CANCER SURVIVAL 
690 1 0 |a CLONOGENIC ASSAY 
690 1 0 |a DNA DETERMINATION 
690 1 0 |a DNA STRAND BREAKAGE 
690 1 0 |a LINEAR ENERGY TRANSFER 
690 1 0 |a LOW ENERGY RADIATION 
690 1 0 |a MELANOMA 
690 1 0 |a MELANOMA CELL 
690 1 0 |a MOUSE 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN DETERMINATION 
690 1 0 |a PROTEIN PHOSPHORYLATION 
690 1 0 |a PROTON RADIATION 
690 1 0 |a RADIATION DOSE 
690 1 0 |a BIOLOGICAL MARKERS 
690 1 0 |a CELL SURVIVAL 
690 1 0 |a DNA BREAKS, DOUBLE-STRANDED 
690 1 0 |a DNA REPAIR 
690 1 0 |a DOSE-RESPONSE RELATIONSHIP, RADIATION 
690 1 0 |a HISTONES 
690 1 0 |a HUMANS 
690 1 0 |a LINEAR ENERGY TRANSFER 
690 1 0 |a LITHIUM 
690 1 0 |a MELANOMA, EXPERIMENTAL 
690 1 0 |a PHOSPHORYLATION 
690 1 0 |a PROTONS 
690 1 0 |a RADIATION TOLERANCE 
690 1 0 |a RADIOISOTOPES 
700 1 |a Bracalente, C. 
700 1 |a Molinari, B.L. 
700 1 |a Palmieri, M.A. 
700 1 |a Policastro, L. 
700 1 |a Kreiner, A.J. 
700 1 |a Burlón, Alejandro Alfredo 
700 1 |a Valda, A. 
700 1 |a Navalesi, D. 
700 1 |a Davidson, Jorge 
700 1 |a Davidson, Miguel 
700 1 |a Vázquez, M. 
700 1 |a Ozafrán, M. 
700 1 |a Durán, H. 
773 0 |d 2009  |g v. 74  |h pp. 1226-1235  |k n. 4  |p Int. J. Radiat. Oncol. Biol. Phys.  |x 03603016  |w (AR-BaUEN)CENRE-5314  |t International Journal of Radiation Oncology Biology Physics 
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