Porphyrin biosynthesis in Rhodopseudomonas palustris-XI. Extraction and characterization of δ-aminolevulinate synthetase
1. 1. Although the levels of ALA-S in photosynthetic (Ph) are higher than in aerobic (A) cells, physical and kinetic properties are similar. Optimum reaction pH is 7.5; maximal product formation occurs at 37°C. 2. 2. The enzyme has an absolute requirement for glycine. The addition of cysteine (CySH)...
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1987
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| LEADER | 08431caa a22006737a 4500 | ||
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| 001 | PAPER-852 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203012.0 | ||
| 008 | 190411s1987 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0038600842 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Viale, A.A. | |
| 245 | 1 | 0 | |a Porphyrin biosynthesis in Rhodopseudomonas palustris-XI. Extraction and characterization of δ-aminolevulinate synthetase |
| 260 | |c 1987 | ||
| 270 | 1 | 0 | |m Viale, A.A. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Algranati, Determination of initial rates in enzymic non-linear progress reactions (1963) Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 73, pp. 152-155 | ||
| 504 | |a Bottomley, Smithee, Characterization and measurement of δ-amino laevulinate synthetase in bone marrow cell mitochondria (1968) Biochim. biophys. Acta, 159, pp. 27-37 | ||
| 504 | |a Dzelzkalns, Foley, Beale, δ-Aminolevulinic acid synthase of Euglena gracilis physical and kinetic properties (1982) Archives of Biochemistry and Biophysics, 21 b, pp. 196-203 | ||
| 504 | |a Ebert, Tschudy, Choudry, Chirigos, A simple micromethod for the direct determination of δ-amino [14C]levulinic acid production in murine spleen and liver homogenates (1970) Biochim. biophys. Acta, 208, pp. 236-250 | ||
| 504 | |a Elliot, The estimation of aminoacetone and δ-amino levulinic acid (1960) Biochem. J., 74, pp. 90-94 | ||
| 504 | |a Fanica-Gaignier, Clement-Metral, 5-Aminolevulinic acid synthetase of Rhodopseudomonas sphaeroides Y (1973) Purification and some properties, 40, pp. 13-18. , Eur. J. Biochem | ||
| 504 | |a Harel, Meler, Rosenberg, Synthesis of 5-amino-levulinic acid-[14C] by cell-free preparations from greening maize leaves (1978) Phytochemistry, 17, pp. 1277-1280 | ||
| 504 | |a Juknat, Kotler, Batlle, (1987) Porphyrin biosynthesis in Rhodopseudomonas palustris—IV. Enzymic cyclotetramerization of porphobilinogen into uroporphyrimogens, , In preparation | ||
| 504 | |a Koopmann, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—VI The effect of metals and various other reagents on uroporphyrinogen decarboxylase activity (1987) Int J Biochem, , in press | ||
| 504 | |a Koopmann, Juknat, Batlle, (1987) Porphyrin biosynthesis in Rhodopseudomonas palustris—III. Evidence for aerobic and anaerobic coproporphyrinogenase activities, , In preparation | ||
| 504 | |a Koopmann, Juknat, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—V. Purification of porphyrinogen decarboxylase and some unusual properties (1987) Int. J. Biochem., 18, pp. 935-944 | ||
| 504 | |a Kotler, Fumagalli, Juknat, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—VIII (1987) Purification and properties of deaminase, , Comp. Biochem. Physiol., Part B, in press | ||
| 504 | |a Kotler, Fumagalli, Juknat, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—IX. Deaminase: kinetic studies (1987) Int. J. Biochem., , in press | ||
| 504 | |a Marriot, Neuberger, Tait, Control of δ-aminolaevulinate synthetase activity in Rhodopseudomonas sphaeroides (1969) Biochem. J., 111, pp. 385-394 | ||
| 504 | |a Mauzerall, Granick, The occurence and determination of δ-aminolevulinic acid and porphobilinogen in urine (1955) J. biol. Chem., 219, pp. 435-446 | ||
| 504 | |a Tait, Aminolaevulinate synthetase of Paracoccus denitrificans (1972) Biochem J, 128, p. 32P | ||
| 504 | |a Tait, Aminolaevulinate synthetase of Micrococcus denitrificans (1973) Biochem. J., 131, pp. 389-403 | ||
| 504 | |a Tuboi, Hayasaka, Control of δ-aminolevulinate synthetase activity in Rhodopseudomonoas sphaeroides—II. Requirement of a disulfide compound for the conversion of the inactive form of fraction I to the active form (1972) Archs Biochem. Biophys., 150, pp. 690-697 | ||
| 504 | |a Tuboi, Kim, Kikuchi, Occurence of a specific and reversible inhibitor of δ-aminolevulinate synthetase in extracts of Rhodopseudomonas sphaeroides (1969) Archs Biochem. Biophys., 130, pp. 92-100 | ||
| 504 | |a Vazquez, Buzaleh, Wider, Batlle, Rhodanese isolation purification and some properties (1987) Int J Biochem, , submitted | ||
| 504 | |a Vazquez, Buzaleh, Wider, Batlle, Soluble and immobilized rhodanese—optimal conditions (1987) Eur. J. Biochem., , submitted for publication | ||
| 504 | |a Viale, Wider, Batlle, Biosintesis de bacterioclorofile en Rhodopseudomonas palustris (1980) Rev. Arg. Microbiol., 12, pp. 1-9 | ||
| 504 | |a Viale, Wider, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity (1980) Int. J. Biochem., 12, pp. 729-733 | ||
| 504 | |a Viale, Wider, Batlle, Biosynthesis of porphyrins in Rhodopseudomonas palustris—XI δ-Amino levulinate synthetase switch-off/on regulation (1987) Int J Biochem, , in press | ||
| 504 | |a Wider, Batlle, Tigier, δ-Aminolevulinate synthetase in extracts of cultured soybean cells (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 235, pp. 511-517 | ||
| 504 | |a Yubisui, Yoneyama, δ-Aminolevulinic acid synthetase of Rhodopseudomonas sphaeroides: purification and properties of the enzyme (1972) Archs Biochem. Biophys., 150, pp. 77-85 | ||
| 520 | 3 | |a 1. 1. Although the levels of ALA-S in photosynthetic (Ph) are higher than in aerobic (A) cells, physical and kinetic properties are similar. Optimum reaction pH is 7.5; maximal product formation occurs at 37°C. 2. 2. The enzyme has an absolute requirement for glycine. The addition of cysteine (CySH), glutathione (GSH), cystine or 2-mercaptoethanol to the reaction mixture does not greatly modify activity, instead it diminishes in the presence of ALA, indicating that the ALA-feedback inhibits its own synthesis. 3. 3. The enzyme is not stable when stored at -15°C; however, activity remains constant for at least 18 hr when different preparations are kept at 0-4°C. 4. 4. Evidence of the existence of one or two low molecular weight compounds which stimulate or stabilize ALA-S activity was found by dialysis of crude extracts. 5. 5. Km values for glycine, succinyl-CoA and pyridoxal phosphate were 1.5 x 10-2 M, 1 x 10-5 M and 1 x 10-6 M respectively. 6. 6. Molecular weight was estimated to be 61 65,000 and no indication of active higher molecular weight species or subunits was obtained. 7. 7. Activity of R. palustris ALA-S can be efficiently regulated within the cell by the formation of one or two low molecular weight factors, depending on growth conditions and also through the levels of both glycine and ALA. © 1987. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Aeknowledgements--E. A. Wider and A. M. del C. Baffle hold the post of Scientific Researchers at the National Research CoLmciI of Argentina (CON ICET). This work was supported by grants from CONICET, SECNT, the University of Buenos Aires, the Ministerio de Sa[ud PSblica de la Nacidn and the Banco de la Naci6n Argentina. | ||
| 593 | |a Cátedra de Microbiologia e Immunología y, Buenos Aires, Argentine | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Departamento de Quimíca Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentine | ||
| 700 | 1 | |a Wider, E.A. | |
| 700 | 1 | |a Del C. Batlle, A.M. | |
| 773 | 0 | |d 1987 |g v. 87 |h pp. 607-613 |k n. 3 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03050491_v87_n3_p607_Viale |y Handle |
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