Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditi...
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1987
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| LEADER | 15414caa a22011057a 4500 | ||
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| 001 | PAPER-846 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203012.0 | ||
| 008 | 190411s1987 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-45949126150 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Rossetti, M.V. | |
| 245 | 1 | 0 | |a Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties |
| 260 | |c 1987 | ||
| 270 | 1 | 0 | |m del C. Batlle, A.M.; Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET-Facultad de Ciencias Exactas y Naturales, UBA) Ciudad Universitaria, Pabellón II 20 Piso, 1428 Buenos Aires Argentine, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Battersby, Fookes, Gustafson-Potter, McDonald, Matcham, Biosynthesis of porphyrins and related macrocycles. Part 171.2. Chemical and enzymic transformation of isomeric aminomethylbilanes into uroporphyrinogens: proof that unrearranged bilane is the preferred enzymic substrate and detection of a transient intermediate (1982) Journal of the Chemical Society, Perkin Transactions 1, 1, pp. 2413-2426 | ||
| 504 | |a Battersby, Fookes, Gustafson-Potter, McDonald, Matcham, Biosynthesis of porphyrins and related macrocycles. Part 181.2. Proof by spectroscopy and synthesis that unrearranged hidroxymethylbilane is the product from deaminase and the substrate for cosynthelase in the biosynthesis of uroporphyrinogen III (1982) Journal of the Chemical Society, Perkin Transactions 1, 1, pp. 2427-2444 | ||
| 504 | |a Battersby, Fookes, Matcham, McDonald, Hollenstein, Biosynthesis of porphyrins and related macrocycles Part 20 Purification of deaminase and studies on its mode of action (1983) Journal of the Chemical Society, Perkin Transactions 1, 1, pp. 3031-3040 | ||
| 504 | |a Battersby, Fookes, Matcham, Pandey, Biosynthesis of porphyrins and related macrocycles Part 21 The interaction of deaminase and its products (hydroxyethylbilane) and the relationship between deaminase and cosynthetase (1983) Journal of the Chemical Society, Perkin Transactions 1, 1, pp. 3041-3047 | ||
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| 504 | |a Ebbon, Tait, Studies on S-adenosyl-methionine-magnesium protoporphyrin methyltransferase on Euglena gracilis strain Z (1969) Biochem. J., 3, pp. 573-582 | ||
| 504 | |a Fumagalli, Kotler, Rossetti, Batlle, Human red cell porphobilinogen deaminase (1985) A simpler method of purification and some unusual properties, 17, pp. 485-494. , Int. J. Biochem | ||
| 504 | |a Fumagalli, Rossetti, Juknat, Kotler, Batlle, Estudios sobre la PBG-asa de higado de cerdo (1982) Anal. Assoc. Quim. Arg., 70, pp. 375-382 | ||
| 504 | |a Gassman, Bogorad, Control of chlorophyll production in rapidly greening bean leaves (1967) PLANT PHYSIOLOGY, 42, pp. 774-780 | ||
| 504 | |a Gassman, Bogorad, Studies on the regeneration of protochlorophyllide after brief illumination of etiolated bean leaves (1967) PLANT PHYSIOLOGY, 42, pp. 781-784 | ||
| 504 | |a Granick, Magnesium protoporphyrin mono-ester and proroporphyrin monomethyl ester in chlorophyll biosynthesis (1961) J. biol. Chem., 236, pp. 1168-1172 | ||
| 504 | |a Granick, (1963) Cytodifferential and Macromolecular Synthesis, p. 144. , M. Locke, Academic Press, New York | ||
| 504 | |a Hart, Battersby, Purification and properties of uroporphyrinogen III synthease (cosynthetase) from Euglena gracilis (1985) Biochem. J., 232, pp. 151-160 | ||
| 504 | |a Hart, Leeper, Battersby, Modification of hydroxymethylbilane synthase (porphobilinogen deaminase) by pyridoxal 5′-phosphate (1984) Biochem. J., 222, pp. 93-102 | ||
| 504 | |a Hovenkamp-Obbema, Effect of added aminolaevulinic acid upon synthesis of chlorophyll in Euglena gracilis (1975) Zeitschrift für Pflanzenphysiologie, 75, pp. 1-5 | ||
| 504 | |a Juknat, Biosintesis enzimática de porfirinógenos (1983) Doctoral thesis, , University of Buenos Aires, Argentine | ||
| 504 | |a Juknat, Rossetti, Batlle, Porphyrin biosynthesis in Euglena gracilis IV (1981) An endogenous factor controlling the enzymic synthesis of porphyrinogens and its possible role in the treatment of some porphyrias, 13, pp. 343-353. , Int. J. Biochem | ||
| 504 | |a Kirk, Studies on the dependence of chlorophyll synthesis on protein synthesis in Euglena gracilis, together with a nomogram for determination of chlorophyll concentration (1968) Planta, 78, pp. 200-207 | ||
| 504 | |a Kirk, Allen, Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidiona (1965) Biochim. Biophys. Res. Commun., 21, pp. 523-530 | ||
| 504 | |a Kotler, Fumagalli, Juknat, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris—IX (1986) Deaminase: Kinetics studies, , Int. J. Biochem., in press | ||
| 504 | |a Llambias, Batlle, Porphyrin biosynthesis in soybean callus-V (1970) The porphobilinogen deaminase-uroporphyrinogen III cosynthetase system Kinetics studies, 220, pp. 552-559. , Biochim. biophys. Acta | ||
| 504 | |a Llambias, Batlle, Negative homotropic kinetics of soybean callus porphobilinogen deaminase-uroporphyrinogen III cosynthetase (1970) FEBS Lett., 9, pp. 180-182 | ||
| 504 | |a Llambias, Batlle, Studies on the porphobilinogen deaminase-uroporhyrinogen cosynthetase system of culture soybean cells (1971) Biochem J, 121, pp. 327-340 | ||
| 504 | |a Llambias, Batlle, Porphyrin biosynthesis-VII (1971) Biochim Biophys Acta, 227, pp. 180-191. , Biochim. biophys. Acta | ||
| 504 | |a Llambias, Locascio, Batlle, Porphyrin biosynthesis-IX (1971) Porphobilinogen deaminase-uroporphyrinogen III cosynthetase from avian erythrocytes, 59, pp. 271-278. , Anal. Assoc. Quim. Arg | ||
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| 504 | |a Moore, Labbe, Assays for ALA and PBG determination (1964) Clin. Chem., 10, pp. 1105-1109 | ||
| 504 | |a Price, Vallee, Euglena gracilis a test organism for study of zinc (1962) PLANT PHYSIOLOGY, 37, pp. 428-433 | ||
| 504 | |a Richard, Nigon, La production d'acide δ-aminolévulinique au cours du verdissement d'euglénes etiolées et ses relations avec la photosynthése (1972) C.r. Acad. Sci. Paris, 274-500, pp. 1307-1310 | ||
| 504 | |a Richard, Nigon, La synthése de lácide δ-aminolévulinique et de la chlorophylle lors de léclairement d' Euglena gracilis etiolées (1973) Biochim. biophys. Acta, 313, pp. 130-149 | ||
| 504 | |a Rimington, Spectral absorption coefficients of some porphyrins in the Soret band region (1960) Biochem J, 75, pp. 620-623 | ||
| 504 | |a Rossetti, Batlle, Polypyrrole intermediates in porphyrin biosynthesis (1977) Studies with Euglena gracilis, 8, pp. 277-283. , Int. J. Biochem | ||
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| 504 | |a Rossetti, Juknat, Kotler, Fumagalli, Batlle, Occurrence of multiple molecular forms of porphobilinogenase in diverse organisms: the minimum quaternary structure of porphobilinogenase is a protomer of one deaminase and one isomerase domain (1980) Int. J. Biochem., 12, pp. 761-767 | ||
| 504 | |a Rossetti, Lombardo, Juknat, Araujo, Batlle, Porphyrin biosynthesis in Euglena gracilis—V. Soluble and particulate PBG-ase (1986) Comp. Biochem. Physiol., 85 B, pp. 451-458 | ||
| 504 | |a Sancovich, Batlle, Grinstein, The porphobilinogen deaminase-uroporphyrinogen III cosynthetase system (porphobilinogenase) from bovine liver (1969) Kinetic studies, 3, pp. 223-226. , FEBS Lett | ||
| 504 | |a Sancovich, Ferramola, Batlle, Grinstein, Preparation of porphobilinogen (1970) Methods in Enzymology, Vol XVIIA, pp. 220-222. , M. Tabor, C.W. Tabor, Academic Press, New York | ||
| 504 | |a Sancovich, Ferramola, Batlle, Kivilevich, Grinstein, Studies on cow liver porphobilinogen deaminase (1976) Acta physiol. latinoam., 26, pp. 147-152 | ||
| 504 | |a Stella, Estudios sobre la enzima deltaaminolevúlico dehidrasa (1977) Doctoral thesis, , University of Buenos Aires, Argentine | ||
| 504 | |a Wider, Batlle, del, Tigier, δ-Aminolaevulinate synthetase in extracts of cultured soybean callus (1971) Biochim. biophys. Acta, 253, pp. 511-517 | ||
| 504 | |a Williams, Morgan, McDonald, Battersby, Purification of porphobilinogen deaminase from Euglena gracilis and studies of its kinetics (1981) Biochem. J., 193, pp. 301-310 | ||
| 520 | 3 | |a 1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditions of growth. 3. 3. Some properties of PBGase were studied. The addition of sodium and magnesium salts to the assay system did not modify enzyme activity. Uroporphyrinogen formation was linear with protein over a wide range of enzyme concentration. 4. 4. When activity was expressed on the basis of the amount of porphyrins formed an optimum pH of 7.4 was obtained but when it was expressed in terms of PBG consumed two pH maxima at 7.0 and 8.5 were observed. 5. 5. Direct plots of velocity against PBG concentration, when activity was measured as PBG consumed, were not hyperbolic; double-reciprocal plots were not linear and Hill plots gave a n value of 2, indicating the existence of positive co-operative effects between two binding sites for PBG per molecule of PBGase. © 1987. |l eng | |
| 536 | |a Detalles de la financiación: Secretaria de Ciencia y Tecnica, Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgemems--Maria Victoria Rossetti and Alcira M. del C. BatIle hold the post of Scicntific Researchcs in the Argentine National Council (CONICET); Maria ESsa Lombardo and Susana Correa Garcia are fclIows of CON~CET. This work was supported by grants from CONICET, SECYT, UBA, Secretaria de Salud ?fiblica dd Ministerio de Acddn SociaI y Medio Ambiente and ~anco de la Naci6n Argentina. The technical assistance of Mrs B. Ricillo de Aprea is gratefully acknowledged. | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET-Facultad de Ciencias Exactas y Naturales, UBA) Ciudad Universitaria, Pabellón II 20 Piso, 1428 Buenos Aires Argentine, Argentina | ||
| 700 | 1 | |a Araujo, L.S. | |
| 700 | 1 | |a Lombardo, M.E. | |
| 700 | 1 | |a García, S.C. | |
| 700 | 1 | |a del C. Batlle, A.M. | |
| 773 | 0 | |d 1987 |g v. 87 |h pp. 593-600 |k n. 3 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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